Restriction map for
pF1KB7185
(Circular) (Six-base) MAPSORT of: pF1KB7185.seq Check: 302 from: 1 to: 4445
pF1KB7185 4445 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 433 433
Size: 4445
AccI GT'mk_AC
Cuts at: 864 1483 864
Size: 619 3826
Acc65I G'GTAC_C
Cuts at: 230 1461 230
Size: 1231 3214
AclI AA'CG_TT
Cuts at: 4285 4285
Size: 4445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1136 2270 2702 3449 3717 1136
Size: 1134 432 747 268 1864
Fragments arranged by size:
1864 1134 747 432 268
AfeI AGC'GCT
Cuts at: 745 969 745
Size: 224 4221
AflIII A'CryG_T
Cuts at: 1797 2901 3878 1797
Size: 1104 977 2364
Fragments arranged by size:
2364 1104 977
AgeI A'CCGG_T
Cuts at: 2833 2833
Size: 4445
AleI CACnn'nnGTG
Cuts at: 813 813
Size: 4445
AlwNI CAG_nnn'CTG
Cuts at: 1750 3317 1750
Size: 1567 2878
ApaI G_GGCC'C
Cuts at: 615 615
Size: 4445
ApaLI G'TGCA_C
Cuts at: 3215 3215
Size: 4445
ApoI r'AATT_y
Cuts at: 220 758 1216 1228 1449 3798 4054 220
Size: 538 458 12 221 2349 256 611
Fragments arranged by size:
2349 611 538 458 256 221 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
AvaI C'yCGr_G
Cuts at: 932 1465 932
Size: 533 3912
BamHI G'GATC_C
Cuts at: 1470 1470
Size: 4445
BanI G'GyrC_C
Cuts at: 115 230 807 1461 1875 2123 2158 115
Size: 115 577 654 414 248 35 2402
Fragments arranged by size:
2402 654 577 414 248 115 35
BanII G_rGCy'C
Cuts at: 188 615 1459 2489 188
Size: 427 844 1030 2144
Fragments arranged by size:
2144 1030 844 427
BbeI G_GCGC'C
Cuts at: 1879 2127 1879
Size: 248 4197
BbsI GAAGACnn'nnnn_
Cuts at: 1124 1124
Size: 4445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 829 863 829
Size: 34 4411
BciVI GTATCCnnnnn_n'
Cuts at: 2343 3104 4445 2343
Size: 761 1341 2343
Fragments arranged by size:
2343 1341 761
BclI T'GATC_A
Cuts at: 1965 1965
Size: 4445
BglI GCCn_nnn'nGGC
Cuts at: 936 936
Size: 4445
BglII A'GATC_T
Cuts at: 1960 3997 1960
Size: 2037 2408
BlpI GC'TnA_GC
Cuts at: 705 1557 705
Size: 852 3593
Bme1580I G_kGCm'C
Cuts at: 615 2070 2163 3219 615
Size: 1455 93 1056 1841
Fragments arranged by size:
1841 1455 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1446 1657 1738 2073 4217 1446
Size: 211 81 335 2144 1674
Fragments arranged by size:
2144 1674 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 4445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1621 2500 2992 3290 164
Size: 1457 879 492 298 1319
Fragments arranged by size:
1457 1319 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 454 4149 454
Size: 3695 750
BsaAI yAC'GTr
Cuts at: 1798 1798
Size: 4445
BsaBI GATnn'nnATC
Cuts at: 6 1959 3996 6
Size: 1953 2037 455
Fragments arranged by size:
2037 1953 455
BsaHI Gr'CG_yC
Cuts at: 1876 2124 2826 4322 1876
Size: 248 702 1496 1999
Fragments arranged by size:
1999 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1634 2155 2833 3107 3254 3665 1634
Size: 521 678 274 147 411 2414
Fragments arranged by size:
2414 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 746 1404 1870 2628 3205 3701 746
Size: 658 466 758 577 496 1490
Fragments arranged by size:
1490 758 658 577 496 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 705 235
Size: 470 3975
BsiEI CG_ry'CG
Cuts at: 104 1987 2033 3241 104
Size: 1883 46 1208 1308
Fragments arranged by size:
1883 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 413 601 1385 1459 2237 2427 3219 413
Size: 188 784 74 778 190 792 1639
Fragments arranged by size:
1639 792 784 778 190 188 74
Bsp1286I G_dGCh'C
Cuts at: 188 413 601 615 1385 1459 2070 2163 2237
Size: 225 188 14 770 74 611 93 74
Cuts at: 2237 2427 2489 3219 188
Size: 190 62 730 1414
Fragments arranged by size:
1414 770 730 611 225 190 188 93
74 74 62 14
BspEI T'CCGG_A
Cuts at: 3665 3665
Size: 4445
BspHI T'CATG_A
Cuts at: 3621 3621
Size: 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 433 1495 1709 2011 2392 257
Size: 176 1062 214 302 381 2310
Fragments arranged by size:
2310 1062 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 2737 2791 4275 4440 50
Size: 2687 54 1484 165 55
Fragments arranged by size:
2687 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2357 2357
Size: 4445
BsrFI r'CCGG_y
Cuts at: 2443 2624 2833 2443
Size: 181 209 4055
Fragments arranged by size:
4055 209 181
BssSI C'ACGA_G
Cuts at: 412 2716 3074 412
Size: 2304 358 1783
Fragments arranged by size:
2304 1783 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1380 1822 1380
Size: 442 4003
BstBI TT'CG_AA
Cuts at: 2806 2806
Size: 4445
BstEII G'GTnAC_C
Cuts at: 369 369
Size: 4445
BstXI CCAn_nnnn'nTGG
Cuts at: 678 678
Size: 4445
BstYI r'GATC_y
Cuts at: 1 869 992 1470 1937 1960 2295 2541 3553
Size: 868 123 478 467 23 335 246 1012
Cuts at: 3553 3639 3651 3997 1
Size: 86 12 346 449
Fragments arranged by size:
1012 868 478 467 449 346 335 246
123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 665 665
Size: 4445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1255 2395 2576 2739 1255
Size: 1140 181 163 2961
Fragments arranged by size:
2961 1140 181 163
BtsI GCAGTG_nn'
Cuts at: 347 1688 347
Size: 1341 3104
DraI TTT'AAA
Cuts at: 194 1233 1444 194
Size: 1039 211 3195
Fragments arranged by size:
3195 1039 211
DrdI GACnn_nn'nnGTC
Cuts at: 2151 3009 2151
Size: 858 3587
EaeI y'GGCC_r
Cuts at: 2030 2204 2595 2622 3967 2030
Size: 174 391 27 1345 2508
Fragments arranged by size:
2508 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2030 2030
Size: 4445
EarI CTCTTCn'nnn_
Cuts at: 2468 2678 2468
Size: 210 4235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2653 2963 3109 4255 2653
Size: 310 146 1146 2843
Fragments arranged by size:
2843 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1457 1457
Size: 4445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1136 2270 2702 3449 3717 1136
Size: 1134 432 747 268 1864
Fragments arranged by size:
1864 1134 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 273 1584 273
Size: 1311 3134
EcoRI G'AATT_C
Cuts at: 220 1449 220
Size: 1229 3216
EcoRV GAT'ATC
Cuts at: 453 453
Size: 4445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4445
FspI TGC'GCA
Cuts at: 2226 2226
Size: 4445
HaeII r_GCGC'y
Cuts at: 747 971 1879 2127 3149 4128 747
Size: 224 908 248 1022 979 1064
Fragments arranged by size:
1064 1022 979 908 248 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 486 518 1342 1374 2287 2317 2319 2349 486
Size: 32 824 32 913 30 2 30 2582
Fragments arranged by size:
2582 913 824 32 32 30 30 2
HincII GTy'rAC
Cuts at: 865 1484 865
Size: 619 3826
KasI G'GCGC_C
Cuts at: 1875 2123 1875
Size: 248 4197
KpnI G_GTAC'C
Cuts at: 234 1465 234
Size: 1231 3214
MluI A'CGCG_T
Cuts at: 3878 3878
Size: 4445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3300 3116
Size: 184 4261
MscI TGG'CCA
Cuts at: 2206 2206
Size: 4445
MslI CAynn'nnrTG
Cuts at: 295 655 676 813 2434 2561 295
Size: 360 21 137 1621 127 2179
Fragments arranged by size:
2179 1621 360 137 127 21
MspA1I CmG'CkG
Cuts at: 1556 1870 2230 3243 3488 1556
Size: 314 360 1013 245 2513
Fragments arranged by size:
2513 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2626 2626
Size: 4445
NarI GG'CG_CC
Cuts at: 1876 2124 1876
Size: 248 4197
NgoMIV G'CCGG_C
Cuts at: 2624 2624
Size: 4445
NspI r_CATG'y
Cuts at: 1498 1998 2905 1498
Size: 500 907 3038
Fragments arranged by size:
3038 907 500
PciI A'CATG_T
Cuts at: 2901 2901
Size: 4445
PfoI T'CCnGG_A
Cuts at: 958 958
Size: 4445
PmeI GTTT'AAAC
Cuts at: 1444 1444
Size: 4445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4413
PpuMI rG'GwC_Cy
Cuts at: 273 273
Size: 4445
PsiI TTA'TAA
Cuts at: 655 1199 655
Size: 544 3901
PspOMI G'GGCC_C
Cuts at: 611 611
Size: 4445
PspXI vC'TCGA_Gb
Cuts at: 932 932
Size: 4445
PstI C_TGCA'G
Cuts at: 917 1492 2177 917
Size: 575 685 3185
Fragments arranged by size:
3185 685 575
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4445
PvuII CAG'CTG
Cuts at: 1870 2230 1870
Size: 360 4085
RsrII CG'GwC_CG
Cuts at: 2640 2640
Size: 4445
SacI G_AGCT'C
Cuts at: 1459 1459
Size: 4445
SalI G'TCGA_C
Cuts at: 863 1482 863
Size: 619 3826
SapI GCTCTTCn'nnn_
Cuts at: 2468 2678 2468
Size: 210 4235
SbfI CC_TGCA'GG
Cuts at: 1492 1492
Size: 4445
ScaI AGT'ACT
Cuts at: 3962 3962
Size: 4445
SexAI A'CCwGG_T
Cuts at: 463 463
Size: 4445
SfcI C'TryA_G
Cuts at: 33 505 887 913 1488 2173 3166 3357 33
Size: 472 382 26 575 685 993 191 1121
Fragments arranged by size:
1121 993 685 575 472 382 191 26
SfoI GGC'GCC
Cuts at: 1877 2125 1877
Size: 248 4197
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
SmaI CCC'GGG
Cuts at: 1467 1467
Size: 4445
SmlI C'TyrA_G
Cuts at: 179 932 1600 2515 3007 3269 179
Size: 753 668 915 492 262 1355
Fragments arranged by size:
1355 915 753 668 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4445
SphI G_CATG'C
Cuts at: 1498 1998 1498
Size: 500 3945
SspI AAT'ATT
Cuts at: 845 845
Size: 4445
StyI C'CwwG_G
Cuts at: 554 1257 1579 1933 554
Size: 703 322 354 3066
Fragments arranged by size:
3066 703 354 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2028 2161 2620 2829 2028
Size: 133 459 209 3644
Fragments arranged by size:
3644 459 209 133
TatI w'GTAC_w
Cuts at: 3960 3960
Size: 4445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 414 446 3962 3994 414
Size: 32 3516 32 865
Fragments arranged by size:
3516 865 32 32
Tth111I GACn'n_nGTC
Cuts at: 513 2242 513
Size: 1729 2716
XbaI T'CTAG_A
Cuts at: 1476 1476
Size: 4445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 1433 1940 444
Size: 989 507 2949
Fragments arranged by size:
2949 989 507
XhoI C'TCGA_G
Cuts at: 932 932
Size: 4445
XmaI C'CCGG_G
Cuts at: 1465 1465
Size: 4445
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XmnI ZraI