Restriction map for
pF1KB7177
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BciVI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT14I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MroI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI StyI
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 51 CGCG CG^CG
AcsI 682 AAATTT [GA]^AATT[CT]
AocI 871 CCTCAGG CC^T[ACGT]AGG
ApoI 682 AAATTT [GA]^AATT[CT]
AspI 365 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 313 GGACC G^G[AT]CC
BalI 192 TGGCCA TGG^CCA
BcgI 302 GCATGAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 822 TGATCA T^GATCA
BcnI 740 CCCGG CC^[GC]GG
BsaBI 1430 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 757 GAGCGG CCGCTC(-3/-3)
BstEII 404 GGTCACC G^GT[ACGT]ACC
BstPI 404 GGTCACC G^GT[ACGT]ACC
BstUI 51 CGCG CG^CG
BstXI 565 CCAGATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1043 AGATCC [GA]^GATC[CT]
Bsu36I 871 CCTCAGG CC^T[ACGT]AGG
DsaI 995 CCACGG C^C[GA][CT]GG
Eco81I 871 CCTCAGG CC^T[ACGT]AGG
EcoNI 928 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 404 GGTCACC G^GT[ACGT]ACC
EcoT22I 1433 ATGCAT ATGCA^T
FbaI 822 TGATCA T^GATCA
HapII 741 CCGG C^CGG
HinfI 638 GAATC G^A[ACGT]TC
HpaII 741 CCGG C^CGG
MamI 1430 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1043 AGATCC [GA]^GATC[CT]
MluNI 192 TGGCCA TGG^CCA
MscI 192 TGGCCA TGG^CCA
MslI 1383 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 741 CCGG C^CGG
MvnI 51 CGCG CG^CG
NciI 740 CCCGG CC^[GC]GG
NotI 52 GCGGCCGC GC^GGCCGC
NsiI 1433 ATGCAT ATGCA^T
NspI 392 GCATGT [GA]CATG^[CT]
PflFI 365 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 324 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 950 CTCAAG C^T[CT][GA]AG
SpeI 718 ACTAGT A^CTAGT
SspI 1051 AATATT AAT^ATT
TfiI 638 GAATC G^A[AT]TC
Tsp45I 405 GTCAC ^GT[GC]AC
Tth111I 365 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 324 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1043 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 1044 GATCC GGATC(4/5)
729 GGATC
ApaI 12 GGGCCC GGGCC^C
1342 GGGCCC
AspHI 286 GAGCAC G[AT]GC[AT]^C
1070 GTGCTC
BbeI 133 GGCGCC GGCGC^C
219 GGCGCC
BbsI 702 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BbvCI 146 CCTCAGC CCTCAGC(-5/-2)
167 CCTCAGC
BfaI 719 CTAG C^TAG
1012 CTAG
BglI 88 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
352 GCCAAACTGGC
BpuAI 702 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BsaI 1333 GAGACC GGTCTC(1/5)
21 GGTCTC
BsiHKAI 286 GAGCAC G[AT]GC[AT]^C
1070 GTGCTC
BsmFI 380 GGGAC GGGAC(10/14)
1368 GTCCC
BsrDI 447 CATTGC GCAATG(2/0)
453 CATTGC
BstNI 248 CCTGG CC^[AT]GG
324 CCAGG
DraII 11 AGGGCCC [GA]G^G[ACGT]CC[CT]
12 GGGCCCC
EcoO109I 11 AGGGCCC [GA]G^G[ACGT]CC[CT]
12 GGGCCCC
EcoRII 248 CCTGG ^CC[AT]GG
324 CCAGG
FauI 349 CCCGC CCCGC(4/6)
759 GCGGG
HaeII 133 GGCGCC [GA]GCGC^[CT]
219 GGCGCC
HgaI 365 GACGC GACGC(5/10)
1274 GACGC
HphI 678 GGTGA GGTGA(8/7)
406 TCACC
KasI 133 GGCGCC G^GCGCC
219 GGCGCC
MaeI 719 CTAG C^TAG
1012 CTAG
MaeII 1143 ACGT A^CGT
1220 ACGT
MaeIII 405 GTCAC ^GT[ACGT]AC
1349 GTAAC
MseI 1055 TTAA T^TAA
1385 TTAA
MvaI 248 CCTGG CC^[AT]GG
324 CCAGG
NarI 133 GGCGCC GG^CGCC
219 GGCGCC
SapI 1156 GAAGAGC GCTCTTC(1/4)
501 GCTCTTC
SfcI 665 CTATAG C^T[GA][CT]AG
1151 CTACAG
SfoI 133 GGCGCC GGC^GCC
219 GGCGCC
Tru9I 1055 TTAA T^TAA
1385 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
BanI BanII BbvI BmyI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I Cac8I CfoI Cfr13I DdeI
DpnII EaeI EagI EarI EclXI
Eco52I Fnu4HI FokI HaeIII HhaI
Hin1I HinP1I ItaI Ksp632I MboI
MboII MnlI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TspRI
TthHB8I