Restriction map for pF1KB7177
 (Circular) (Six-base) MAPSORT of: pF1KB7177.seq  Check: 3706  from: 1  to: 4559



pF1KB7177  4559 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:   1597    1597 
   Size:       4559
 
Acc65I G'GTAC_C

Cuts at:   1575    1575 
   Size:       4559
 
AclI AA'CG_TT

Cuts at:   4399    4399 
   Size:       4559
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2384    2816    3563    3831    2384 
   Size:        432     747     268    3112

  Fragments arranged by size: 

                3112     747     432     268
 
AflIII A'CryG_T

Cuts at:   1911    3015    3992    1911 
   Size:       1104     977    2478

  Fragments arranged by size: 

                2478    1104     977
 
AgeI A'CCGG_T

Cuts at:   2947    2947 
   Size:       4559
 
AlwNI CAG_nnn'CTG

Cuts at:   1864    3431    1864 
   Size:       1567    2992
 
ApaI G_GGCC'C

Cuts at:    124    1454     124 
   Size:       1330    3229
 
ApaLI G'TGCA_C

Cuts at:   3329    3329 
   Size:       4559
 
ApoI r'AATT_y

Cuts at:    790    1563    3912    4168     790 
   Size:        773    2349     256    1181

  Fragments arranged by size: 

                2349    1181     773     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4559
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4559
 
AvaI C'yCGr_G

Cuts at:    124     237     372    1579     124 
   Size:        113     135    1207    3104

  Fragments arranged by size: 

                3104    1207     135     113
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    412     445     412 
   Size:         33    4526
 
BamHI G'GATC_C

Cuts at:   1584    1584 
   Size:       4559
 
BanI G'GyrC_C

Cuts at:    241     327     441    1575    1989    2237    2272     241 
   Size:         86     114    1134     414     248      35    2528

  Fragments arranged by size: 

                2528    1134     414     248     114      86      35
 
BanII G_rGCy'C

Cuts at:    124     379    1454    1573    2603     124 
   Size:        255    1075     119    1030    2080

  Fragments arranged by size: 

                2080    1075    1030     255     119
 
BbeI G_GCGC'C

Cuts at:    245     331    1993    2241     245 
   Size:         86    1662     248    2563

  Fragments arranged by size: 

                2563    1662     248      86
 
BbsI GAAGACnn'nnnn_

Cuts at:    817    1517     817 
   Size:        700    3859
 
BbvCI CC'TCA_GC

Cuts at:    255     276     255 
   Size:         21    4538
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    399     433     399 
   Size:         34    4525
 
BciVI GTATCCnnnnn_n'

Cuts at:   2457    3218    4559    2457 
   Size:        761    1341    2457

  Fragments arranged by size: 

                2457    1341     761
 
BclI T'GATC_A

Cuts at:    930    2079     930 
   Size:       1149    3410
 
BfrBI ATG'CAT

Cuts at:   1543    1543 
   Size:       4559
 
BglI GCCn_nnn'nGGC

Cuts at:    202     466     202 
   Size:        264    4295
 
BglII A'GATC_T

Cuts at:   2074    4111    2074 
   Size:       2037    2522
 
BlpI GC'TnA_GC

Cuts at:   1671    1671 
   Size:       4559
 
Bme1580I G_kGCm'C

Cuts at:    124     446    1454    2184    2277    3333     124 
   Size:        322    1008     730      93    1056    1350

  Fragments arranged by size: 

                1350    1056    1008     730     322      93
 
BmgBI CAC'GTC

Cuts at:   1329    1329 
   Size:       4559
 
BmrI ACTGGGnnnn_n'

Cuts at:    420    1448    1771    1852    2187    4331     420 
   Size:       1028     323      81     335    2144     648

  Fragments arranged by size: 

                2144    1028     648     335     323      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2926    2926 
   Size:       4559
 
Bpu10I CC'TnA_GC

Cuts at:    255     276     255 
   Size:         21    4538
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1043    1735    2614    3106    3404    1043 
   Size:        692     879     492     298    2198

  Fragments arranged by size: 

                2198     879     692     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    135    1435    4263     135 
   Size:       1300    2828     431

  Fragments arranged by size: 

                2828    1300     431
 
BsaAI yAC'GTr

Cuts at:   1912    1912 
   Size:       4559
 
BsaBI GATnn'nnATC

Cuts at:      6    1542    2073    4110       6 
   Size:       1536     531    2037     455

  Fragments arranged by size: 

                2037    1536     531     455
 
BsaHI Gr'CG_yC

Cuts at:    242     328    1383    1990    2238    2940    4436     242 
   Size:         86    1055     607     248     702    1496     365

  Fragments arranged by size: 

                1496    1055     702     607     365     248      86
 
BsaWI w'CCGG_w

Cuts at:   1748    2269    2947    3221    3368    3779    1748 
   Size:        521     678     274     147     411    2528

  Fragments arranged by size: 

                2528     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    243     406     886     243 
   Size:        163     480    3916

  Fragments arranged by size: 

                3916     480     163
 
BseYI C'CCAG_C

Cuts at:   1984    2742    3319    3815    1984 
   Size:        758     577     496    2728

  Fragments arranged by size: 

                2728     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     164     206     266    2101    2147    3355     104 
   Size:         60      42      60    1835      46    1208    1308

  Fragments arranged by size: 

                1835    1308    1208      60      60      46      42
 
BsiHKAI G_wGCw'C

Cuts at:    398    1182    1573    2351    2541    3333     398 
   Size:        784     391     778     190     792    1624

  Fragments arranged by size: 

                1624     792     784     778     391     190
 
Bsp1286I G_dGCh'C

Cuts at:    124     379     398     446    1182    1454    1573    2184    2277 
   Size:        255      19      48     736     272     119     611      93

Cuts at:   2277    2351    2541    2603    3333     124 
   Size:         74     190      62     730    1350

  Fragments arranged by size: 

                1350     736     730     611     272     255     190     119
                  93      74      62      48      19
 
BspEI T'CCGG_A

Cuts at:   3779    3779 
   Size:       4559
 
BspHI T'CATG_A

Cuts at:   3735    3735 
   Size:       4559
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1609    1823    2125    2506    1609 
   Size:        214     302     381    3662

  Fragments arranged by size: 

                3662     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     867    2851    2905    4389    4554      50 
   Size:        817    1984      54    1484     165      55

  Fragments arranged by size: 

                1984    1484     817     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    554     560    2471     554 
   Size:          6    1911    2642

  Fragments arranged by size: 

                2642    1911       6
 
BsrFI r'CCGG_y

Cuts at:   2557    2738    2947    2557 
   Size:        181     209    4169

  Fragments arranged by size: 

                4169     209     181
 
BssSI C'ACGA_G

Cuts at:   2830    3188    2830 
   Size:        358    4201
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1936    1936 
   Size:       4559
 
BstBI TT'CG_AA

Cuts at:   2920    2920 
   Size:       4559
 
BstEII G'GTnAC_C

Cuts at:    512     512 
   Size:       4559
 
BstXI CCAn_nnnn'nTGG

Cuts at:    680     680 
   Size:       4559
 
BstYI r'GATC_y

Cuts at:      1    1151    1584    2051    2074    2409    2655    3667    3753 
   Size:       1150     433     467      23     335     246    1012      86

Cuts at:   3753    3765    4111       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1150    1012     467     449     433     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    980     980 
   Size:       4559
 
BtgI C'CryG_G

Cuts at:    107    1103     107 
   Size:        996    3563
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2509    2690    2853    2509 
   Size:        181     163    4215

  Fragments arranged by size: 

                4215     181     163
 
BtsI GCAGTG_nn'

Cuts at:    227    1091    1802     227 
   Size:        864     711    2984

  Fragments arranged by size: 

                2984     864     711
 
DraI TTT'AAA

Cuts at:   1558    1558 
   Size:       4559
 
DrdI GACnn_nn'nnGTC

Cuts at:   2265    3123    2265 
   Size:        858    3701
 
EaeI y'GGCC_r

Cuts at:    161     203     263     300    1302    2144    2318    2709    2736 
   Size:         42      60      37    1002     842     174     391      27

Cuts at:   2736    4081     161 
   Size:       1345     639

  Fragments arranged by size: 

                1345    1002     842     639     391     174      60      42
                  37      27
 
EagI C'GGCC_G

Cuts at:    161     203     263    2144     161 
   Size:         42      60    1881    2576

  Fragments arranged by size: 

                2576    1881      60      42
 
EarI CTCTTCn'nnn_

Cuts at:    616     887    1259    1433    1499    2582    2792     616 
   Size:        271     372     174      66    1083     210    2383

  Fragments arranged by size: 

                2383    1083     372     271     210     174      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    159     165     180    2767    3077    3223    4369     159 
   Size:          6      15    2587     310     146    1146     349

  Fragments arranged by size: 

                2587    1146     349     310     146      15       6
 
EcoICRI GAG'CTC

Cuts at:   1571    1571 
   Size:       4559
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2384    2816    3563    3831    2384 
   Size:        432     747     268    3112

  Fragments arranged by size: 

                3112     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1040    1040 
   Size:       4559
 
EcoO109I rG'GnC_Cy

Cuts at:    120     121    1698     120 
   Size:          1    1577    2981

  Fragments arranged by size: 

                2981    1577       1
 
EcoRI G'AATT_C

Cuts at:   1563    1563 
   Size:       4559
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1210    1288    2578    1210 
   Size:         78    1290    3191

  Fragments arranged by size: 

                3191    1290      78
 
FspI TGC'GCA

Cuts at:   2340    2340 
   Size:       4559
 
HaeII r_GCGC'y

Cuts at:    245     331    1993    2241    3263    4242     245 
   Size:         86    1662     248    1022     979     562

  Fragments arranged by size: 

                1662    1022     979     562     248      86
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    963     995    2401    2431    2433    2463     963 
   Size:         32    1406      30       2      30    3059

  Fragments arranged by size: 

                3059    1406      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1598    1598 
   Size:       4559
 
KasI G'GCGC_C

Cuts at:    241     327    1989    2237     241 
   Size:         86    1662     248    2563

  Fragments arranged by size: 

                2563    1662     248      86
 
KpnI G_GTAC'C

Cuts at:   1579    1579 
   Size:       4559
 
MluI A'CGCG_T

Cuts at:   3992    3992 
   Size:       4559
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    399     524     774    1234    3230    3414     399 
   Size:        125     250     460    1996     184    1544

  Fragments arranged by size: 

                1996    1544     460     250     184     125
 
MscI TGG'CCA

Cuts at:    302    2320     302 
   Size:       2018    2541
 
MslI CAynn'nnrTG

Cuts at:   1495    2548    2675    1495 
   Size:       1053     127    3379

  Fragments arranged by size: 

                3379    1053     127
 
MspA1I CmG'CkG

Cuts at:   1670    1984    2344    3357    3602    1670 
   Size:        314     360    1013     245    2627

  Fragments arranged by size: 

                2627    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2740    2740 
   Size:       4559
 
NarI GG'CG_CC

Cuts at:    242     328    1990    2238     242 
   Size:         86    1662     248    2563

  Fragments arranged by size: 

                2563    1662     248      86
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4559
 
NgoMIV G'CCGG_C

Cuts at:   2738    2738 
   Size:       4559
 
NotI GC'GGCC_GC

Cuts at:    161     161 
   Size:       4559
 
NsiI A_TGCA'T

Cuts at:   1545    1545 
   Size:       4559
 
NspI r_CATG'y

Cuts at:    504    1612    2112    3019     504 
   Size:       1108     500     907    2044

  Fragments arranged by size: 

                2044    1108     907     500
 
PciI A'CATG_T

Cuts at:   3015    3015 
   Size:       4559
 
PflMI CCAn_nnn'nTGG

Cuts at:    438     438 
   Size:       4559
 
PmeI GTTT'AAAC

Cuts at:   1558    1558 
   Size:       4559
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3698    3730    3698 
   Size:         32    4527
 
PspOMI G'GGCC_C

Cuts at:    120    1450     120 
   Size:       1330    3229
 
PstI C_TGCA'G

Cuts at:   1606    2291    1606 
   Size:        685    3874
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4559
 
PvuII CAG'CTG

Cuts at:   1984    2344    1984 
   Size:        360    4199
 
RsrII CG'GwC_CG

Cuts at:   2754    2754 
   Size:       4559
 
SacI G_AGCT'C

Cuts at:   1573    1573 
   Size:       4559
 
SalI G'TCGA_C

Cuts at:   1596    1596 
   Size:       4559
 
SapI GCTCTTCn'nnn_

Cuts at:    616    1259    2582    2792     616 
   Size:        643    1323     210    2383

  Fragments arranged by size: 

                2383    1323     643     210
 
SbfI CC_TGCA'GG

Cuts at:   1606    1606 
   Size:       4559
 
ScaI AGT'ACT

Cuts at:   4076    4076 
   Size:       4559
 
SfcI C'TryA_G

Cuts at:     33     773    1259    1602    2287    3280    3471      33 
   Size:        740     486     343     685     993     191    1121

  Fragments arranged by size: 

                1121     993     740     685     486     343     191
 
SfoI GGC'GCC

Cuts at:    243     329    1991    2239     243 
   Size:         86    1662     248    2563

  Fragments arranged by size: 

                2563    1662     248      86
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4559
 
SmaI CCC'GGG

Cuts at:   1581    1581 
   Size:       4559
 
SmlI C'TyrA_G

Cuts at:   1058    1714    2629    3121    3383    1058 
   Size:        656     915     492     262    2234

  Fragments arranged by size: 

                2234     915     656     492     262
 
SpeI A'CTAG_T

Cuts at:     67     826      67 
   Size:        759    3800
 
SphI G_CATG'C

Cuts at:   1612    2112    1612 
   Size:        500    4059
 
SspI AAT'ATT

Cuts at:   1161    1161 
   Size:       4559
 
StyI C'CwwG_G

Cuts at:    107    1693    2047     107 
   Size:       1586     354    2619

  Fragments arranged by size: 

                2619    1586     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    531    2142    2275    2734    2943     531 
   Size:       1611     133     459     209    2147

  Fragments arranged by size: 

                2147    1611     459     209     133
 
TatI w'GTAC_w

Cuts at:   4074    4074 
   Size:       4559
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4076    4108    4076 
   Size:         32    4527
 
Tth111I GACn'n_nGTC

Cuts at:    476    2356     476 
   Size:       1880    2679
 
XbaI T'CTAG_A

Cuts at:   1590    1590 
   Size:       4559
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2054    2054 
   Size:       4559
 
XmaI C'CCGG_G

Cuts at:   1579    1579 
   Size:       4559
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NsiI
     NspI     PciI    PflMI     PmeI     PpiI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BmtI     BpmI    BsaXI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII  BstZ17I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NruI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     StuI     SwaI     XhoI     XmnI     ZraI
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