Restriction map for pF1KB7158
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AccII       AccIII      AclI        AcsI        AflII       
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp700I     
AspEI       AspHI       AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DpnII       DraI        DraIII      DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KspI        
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         547  GACGTC               GACGT^C
Acc65I        425  GGTACC               G^GTACC
AccI           90  GTCTAC               GT^[AC][GT]AC
AcyI          547  GACGTC               G[GA]^CG[CT]C
AfaI          426  GTAC                 GT^AC
AflIII        380  ACATGT               A^C[GA][CT]GT
AgeI          968  ACCGGT               A^CCGGT
AlwNI         773  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          859  ATTAAT               AT^TAAT
AsnI          859  ATTAAT               AT^TAAT
Asp718I       425  GGTACC               G^GTACC
AspI          754  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          425  GGTACC               G^G[CT][GA]CC
BsaHI         547  GACGTC               G[GA]^CG[CT]C
BsaI         1075  GAGACC               GGTCTC(1/5)
BsaWI         968  ACCGGT               [AT]^CCGG[AT]
BsmBI         545  GAGACG               CGTCTC(1/5)
BsmI          576  GAATGC               GAATGC(1/-1)
BspLU11I      380  ACATGT               A^CATGT
BspMI         406  ACCTGC               ACCTGC(4/8)
BsrDI         524  CATTGC               GCAATG(2/0)
BsrFI         968  ACCGGT               [GA]^CCGG[CT]
BsrI          326  ACTGG                ACTGG(1/-1)
Cfr10I        968  ACCGGT               [GA]^CCGG[CT]
DraII         219  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI         1077  GACCTTTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          287  CTCTTC               CTCTTC(1/4)
EcoO109I      219  GGGTCCT              [GA]G^G[ACGT]CC[CT]
Hin1I         547  GACGTC               G[GA]^CG[CT]C
KpnI          425  GGTACC               GGTAC^C
Ksp632I       287  CTCTTC               CTCTTC(1/4)
MaeII         548  ACGT                 A^CGT
PflFI         754  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         968  ACCGGT               A^CCGGT
PleI           88  GAGTC                GAGTC(4/5)
PpuMI         219  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         859  ATTAAT               AT^TAAT
RsaI          426  GTAC                 GT^AC
TaqI          543  TCGA                 T^CGA
TfiI          920  GATTC                G^A[AT]TC
Tsp45I        752  GTGAC                ^GT[GC]AC
Tth111I       754  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       543  TCGA                 T^CGA
XcmI          615  CCAATGGTCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          987  CCGC                 CCGC(-3/-1)
               47  GCGG                
AvaII         220  GGTCC                G^G[AT]CC
              337  GGACC               
BbsI          717  GAAGAC               GAAGAC(2/6)
               50  GTCTTC              
BfaI           29  CTAG                 C^TAG
              236  CTAG                
BpuAI         717  GAAGAC               GAAGAC(2/6)
               50  GTCTTC              
BstXI         908  CCATCCTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1006  CCAATTACTTGG        
Cfr13I        220  GGTCC                G^G[ACGT]CC
              337  GGACC               
EcoT14I       651  CCTTGG               C^C[AT][AT]GG
             1020  CCAAGG              
HapII         969  CCGG                 C^CGG
             1047  CCGG                
HinfI          88  GAGTC                G^A[ACGT]TC
              920  GATTC               
HpaII         969  CCGG                 C^CGG
             1047  CCGG                
HphI          404  TCACC                GGTGA(8/7)
              923  TCACC               
MaeI           29  CTAG                 C^TAG
              236  CTAG                
MaeIII        196  GTTAC                ^GT[ACGT]AC
              752  GTGAC               
MslI          127  CATTTAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              405  CACCTGCATG          
MspI          969  CCGG                 C^CGG
             1047  CCGG                
MwoI          625  GCTGGGATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1046  GCCGGAAAAGC         
NspI          380  ACATGT               [GA]CATG^[CT]
              685  ACATGC              
Sau96I        220  GGTCC                G^G[ACGT]CC
              337  GGACC               
SspI          184  AATATT               AAT^ATT
              672  AATATT              
StyI          651  CCTTGG               C^C[AT][AT]GG
             1020  CCAAGG              
TspRI         200  CACTG                CA[GC]TG[ACGT][ACGT]^
              990  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BsmAI       BssKI       BstF5I      BstNI       Cac8I       
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
ItaI        MboII       MnlI        MseI        MspA1I      
MvaI        NlaIII      NlaIV       PvuII       ScrFI       
SfaNI       Tru9I       TseI        Tsp509I     
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