Restriction map for pF1KB7156
 (Circular) (Six-base) MAPSORT of: pF1KB7156.seq  Check: 8622  from: 1  to: 4190



pF1KB7156  4190 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    559     559 
   Size:       4190
 
AccI GT'mk_AC

Cuts at:    365    1228     365 
   Size:        863    3327
 
Acc65I G'GTAC_C

Cuts at:   1206    1206 
   Size:       4190
 
AclI AA'CG_TT

Cuts at:   4030    4030 
   Size:       4190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    149     304     404    2015    2447    3194    3462     149 
   Size:        155     100    1611     432     747     268     877

  Fragments arranged by size: 

                1611     877     747     432     268     155     100
 
AflIII A'CryG_T

Cuts at:    466    1542    2646    3623     466 
   Size:       1076    1104     977    1033

  Fragments arranged by size: 

                1104    1076    1033     977
 
AgeI A'CCGG_T

Cuts at:   2578    2578 
   Size:       4190
 
AlwNI CAG_nnn'CTG

Cuts at:   1495    3062    1495 
   Size:       1567    2623
 
ApaLI G'TGCA_C

Cuts at:   2960    2960 
   Size:       4190
 
ApoI r'AATT_y

Cuts at:    312     669     788    1030    1194    3543    3799     312 
   Size:        357     119     242     164    2349     256     703

  Fragments arranged by size: 

                2349     703     357     256     242     164     119
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4190
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4190
 
AvaI C'yCGr_G

Cuts at:   1210    1210 
   Size:       4190
 
BamHI G'GATC_C

Cuts at:   1215    1215 
   Size:       4190
 
BanI G'GyrC_C

Cuts at:   1206    1620    1868    1903    1206 
   Size:        414     248      35    3493

  Fragments arranged by size: 

                3493     414     248      35
 
BanII G_rGCy'C

Cuts at:   1204    2234    1204 
   Size:       1030    3160
 
BbeI G_GCGC'C

Cuts at:   1624    1872    1624 
   Size:        248    3942
 
BbsI GAAGACnn'nnnn_

Cuts at:    292     292 
   Size:       4190
 
BciVI GTATCCnnnnn_n'

Cuts at:   2088    2849    4190    2088 
   Size:        761    1341    2088

  Fragments arranged by size: 

                2088    1341     761
 
BclI T'GATC_A

Cuts at:    734    1710     734 
   Size:        976    3214
 
BglII A'GATC_T

Cuts at:   1705    3742    1705 
   Size:       2037    2153
 
BlpI GC'TnA_GC

Cuts at:   1302    1302 
   Size:       4190
 
Bme1580I G_kGCm'C

Cuts at:   1815    1908    2964    1815 
   Size:         93    1056    3041

  Fragments arranged by size: 

                3041    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    721    1402    1483    1818    3962     721 
   Size:        681      81     335    2144     949

  Fragments arranged by size: 

                2144     949     681     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2557    2557 
   Size:       4190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1033    1033 
   Size:       4190
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1366    2245    2737    3035    1366 
   Size:        879     492     298    2521

  Fragments arranged by size: 

                2521     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3894    3894 
   Size:       4190
 
BsaAI yAC'GTr

Cuts at:    467     922    1543     467 
   Size:        455     621    3114

  Fragments arranged by size: 

                3114     621     455
 
BsaBI GATnn'nnATC

Cuts at:      6    1704    3741       6 
   Size:       1698    2037     455

  Fragments arranged by size: 

                2037    1698     455
 
BsaHI Gr'CG_yC

Cuts at:   1621    1869    2571    4067    1621 
   Size:        248     702    1496    1744

  Fragments arranged by size: 

                1744    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1379    1900    2578    2852    2999    3410    1379 
   Size:        521     678     274     147     411    2159

  Fragments arranged by size: 

                2159     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    690    1615    2373    2950    3446     690 
   Size:        925     758     577     496    1434

  Fragments arranged by size: 

                1434     925     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1148    1148 
   Size:       4190
 
BsiEI CG_ry'CG

Cuts at:    104    1732    1778    2986     104 
   Size:       1628      46    1208    1308

  Fragments arranged by size: 

                1628    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1114    1204    1982    2172    2964    1114 
   Size:         90     778     190     792    2340

  Fragments arranged by size: 

                2340     792     778     190      90
 
Bsp1286I G_dGCh'C

Cuts at:   1114    1204    1815    1908    1982    2172    2234    2964    1114 
   Size:         90     611      93      74     190      62     730    2340

  Fragments arranged by size: 

                2340     730     611     190      93      90      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3410    3410 
   Size:       4190
 
BspHI T'CATG_A

Cuts at:    194    3366     194 
   Size:       3172    1018
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    559    1240    1454    1756    2137     559 
   Size:        681     214     302     381    2612

  Fragments arranged by size: 

                2612     681     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2482    2536    4020    4185      50 
   Size:       2432      54    1484     165      55

  Fragments arranged by size: 

                2432    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2102    2102 
   Size:       4190
 
BsrFI r'CCGG_y

Cuts at:   2188    2369    2578    2188 
   Size:        181     209    3800

  Fragments arranged by size: 

                3800     209     181
 
BssSI C'ACGA_G

Cuts at:   2461    2819    2461 
   Size:        358    3832
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1567    1567 
   Size:       4190
 
BstBI TT'CG_AA

Cuts at:   2551    2551 
   Size:       4190
 
BstYI r'GATC_y

Cuts at:      1    1215    1682    1705    2040    2286    3298    3384    3396 
   Size:       1214     467      23     335     246    1012      86      12

Cuts at:   3396    3742       1 
   Size:        346     449

  Fragments arranged by size: 

                1214    1012     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2140    2321    2484    2140 
   Size:        181     163    3846

  Fragments arranged by size: 

                3846     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1433    1433 
   Size:       4190
 
ClaI AT'CG_AT

Cuts at:    483     483 
   Size:       4190
 
DraI TTT'AAA

Cuts at:   1035    1189    1035 
   Size:        154    4036
 
DrdI GACnn_nn'nnGTC

Cuts at:   1896    2754    1896 
   Size:        858    3332
 
EaeI y'GGCC_r

Cuts at:    293     581     953    1775    1949    2340    2367    3712     293 
   Size:        288     372     822     174     391      27    1345     771

  Fragments arranged by size: 

                1345     822     771     391     372     288     174      27
            
 
EagI C'GGCC_G

Cuts at:   1775    1775 
   Size:       4190
 
EarI CTCTTCn'nnn_

Cuts at:    126    2213    2423     126 
   Size:       2087     210    1893

  Fragments arranged by size: 

                2087    1893     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2398    2708    2854    4000    2398 
   Size:        310     146    1146    2588

  Fragments arranged by size: 

                2588    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1202    1202 
   Size:       4190
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    149     304     404    1033    2015    2447    3194    3462     149 
   Size:        155     100     629     982     432     747     268     877

  Fragments arranged by size: 

                 982     877     747     629     432     268     155     100
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    775     775 
   Size:       4190
 
EcoO109I rG'GnC_Cy

Cuts at:    768    1329     768 
   Size:        561    3629
 
EcoRI G'AATT_C

Cuts at:   1194    1194 
   Size:       4190
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2209    2209 
   Size:       4190
 
FspI TGC'GCA

Cuts at:   1971    1971 
   Size:       4190
 
HaeII r_GCGC'y

Cuts at:   1624    1872    2894    3873    1624 
   Size:        248    1022     979    1941

  Fragments arranged by size: 

                1941    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1031    1063    2032    2062    2064    2094    1031 
   Size:         32     969      30       2      30    3127

  Fragments arranged by size: 

                3127     969      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1229    1229 
   Size:       4190
 
KasI G'GCGC_C

Cuts at:   1620    1868    1620 
   Size:        248    3942
 
KpnI G_GTAC'C

Cuts at:   1210    1210 
   Size:       4190
 
MfeI C'AATT_G

Cuts at:    839     990     839 
   Size:        151    4039
 
MluI A'CGCG_T

Cuts at:   3623    3623 
   Size:       4190
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2861    3045    2861 
   Size:        184    4006
 
MscI TGG'CCA

Cuts at:    295     583    1951     295 
   Size:        288    1368    2534

  Fragments arranged by size: 

                2534    1368     288
 
MslI CAynn'nnrTG

Cuts at:    223     373     608     955    2179    2306     223 
   Size:        150     235     347    1224     127    2107

  Fragments arranged by size: 

                2107    1224     347     235     150     127
 
MspA1I CmG'CkG

Cuts at:    386    1301    1615    1975    2988    3233     386 
   Size:        915     314     360    1013     245    1343

  Fragments arranged by size: 

                1343    1013     915     360     314     245
 
NaeI GCC'GGC

Cuts at:   2371    2371 
   Size:       4190
 
NarI GG'CG_CC

Cuts at:   1621    1869    1621 
   Size:        248    3942
 
NgoMIV G'CCGG_C

Cuts at:   2369    2369 
   Size:       4190
 
NspI r_CATG'y

Cuts at:   1243    1743    2650    1243 
   Size:        500     907    2783

  Fragments arranged by size: 

                2783     907     500
 
PciI A'CATG_T

Cuts at:   2646    2646 
   Size:       4190
 
PfoI T'CCnGG_A

Cuts at:    862     862 
   Size:       4190
 
PmeI GTTT'AAAC

Cuts at:   1189    1189 
   Size:       4190
 
PmlI CAC'GTG

Cuts at:    467     467 
   Size:       4190
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3329    3361    3329 
   Size:         32    4158
 
PstI C_TGCA'G

Cuts at:   1237    1922    1237 
   Size:        685    3505
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4190
 
PvuII CAG'CTG

Cuts at:   1615    1975    1615 
   Size:        360    3830
 
RsrII CG'GwC_CG

Cuts at:   2385    2385 
   Size:       4190
 
SacI G_AGCT'C

Cuts at:   1204    1204 
   Size:       4190
 
SalI G'TCGA_C

Cuts at:   1227    1227 
   Size:       4190
 
SapI GCTCTTCn'nnn_

Cuts at:   2213    2423    2213 
   Size:        210    3980
 
SbfI CC_TGCA'GG

Cuts at:   1237    1237 
   Size:       4190
 
ScaI AGT'ACT

Cuts at:   3707    3707 
   Size:       4190
 
SfcI C'TryA_G

Cuts at:     33     927    1233    1918    2911    3102      33 
   Size:        894     306     685     993     191    1121

  Fragments arranged by size: 

                1121     993     894     685     306     191
 
SfoI GGC'GCC

Cuts at:   1622    1870    1622 
   Size:        248    3942
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4190
 
SmaI CCC'GGG

Cuts at:   1212    1212 
   Size:       4190
 
SmlI C'TyrA_G

Cuts at:   1345    2260    2752    3014    1345 
   Size:        915     492     262    2521

  Fragments arranged by size: 

                2521     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4190
 
SphI G_CATG'C

Cuts at:   1243    1743    1243 
   Size:        500    3690
 
SspI AAT'ATT

Cuts at:    236     636     881     236 
   Size:        400     245    3545

  Fragments arranged by size: 

                3545     400     245
 
StyI C'CwwG_G

Cuts at:   1324    1678    1324 
   Size:        354    3836
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    518    1773    1906    2365    2574     518 
   Size:       1255     133     459     209    2134

  Fragments arranged by size: 

                2134    1255     459     209     133
 
TatI w'GTAC_w

Cuts at:   3705    3705 
   Size:       4190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3707    3739    3707 
   Size:         32    4158
 
Tth111I GACn'n_nGTC

Cuts at:   1987    1987 
   Size:       4190
 
XbaI T'CTAG_A

Cuts at:   1221    1221 
   Size:       4190
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    708    1685     708 
   Size:        977    3213
 
XmaI C'CCGG_G

Cuts at:   1210    1210 
   Size:       4190
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI
     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI
     PmeI     PmlI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    CspCI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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