Restriction map for pF1KB7156
 (Linear) (Six-base) MAPSORT of: KIBB7156.seq  Check: 8870  from: 1  to: 1077



KIBB7156.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     451    1077 
   Size:        451     626
 
AccI GT'mk_AC

Cuts at:      0     257    1077 
   Size:        257     820
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     196     296    1077 
   Size:         41     155     100     781

  Fragments arranged by size: 

                 781     155     100      41
 
AflIII A'CryG_T

Cuts at:      0     358    1077 
   Size:        358     719
 
ApoI r'AATT_y

Cuts at:      0     204     561     680     922    1077 
   Size:        204     357     119     242     155

  Fragments arranged by size: 

                 357     242     204     155     119
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     184    1077 
   Size:        184     893
 
BclI T'GATC_A

Cuts at:      0     626    1077 
   Size:        626     451
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     613    1077 
   Size:        613     464
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     925    1077 
   Size:        925     152
 
BsaAI yAC'GTr

Cuts at:      0     359     814    1077 
   Size:        359     455     263

  Fragments arranged by size: 

                 455     359     263
 
BseYI C'CCAG_C

Cuts at:      0     582    1077 
   Size:        582     495
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1040    1077 
   Size:       1040      37
 
BsiHKAI G_wGCw'C

Cuts at:      0    1006    1077 
   Size:       1006      71
 
Bsp1286I G_dGCh'C

Cuts at:      0    1006    1077 
   Size:       1006      71
 
BspHI T'CATG_A

Cuts at:      0      86    1077 
   Size:         86     991
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     451    1077 
   Size:        451     626
 
ClaI AT'CG_AT

Cuts at:      0     375    1077 
   Size:        375     702
 
DraI TTT'AAA

Cuts at:      0     927    1077 
   Size:        927     150
 
EaeI y'GGCC_r

Cuts at:      0     185     473     845    1077 
   Size:        185     288     372     232

  Fragments arranged by size: 

                 372     288     232     185
 
EarI CTCTTCn'nnn_

Cuts at:      0      18    1077 
   Size:         18    1059
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     196     296     925    1077 
   Size:         41     155     100     629     152

  Fragments arranged by size: 

                 629     155     152     100      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     667    1077 
   Size:        667     410
 
EcoO109I rG'GnC_Cy

Cuts at:      0     660    1077 
   Size:        660     417
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     923     955    1077 
   Size:        923      32     122

  Fragments arranged by size: 

                 923     122      32
 
MfeI C'AATT_G

Cuts at:      0     731     882    1077 
   Size:        731     151     195

  Fragments arranged by size: 

                 731     195     151
 
MscI TGG'CCA

Cuts at:      0     187     475    1077 
   Size:        187     288     602

  Fragments arranged by size: 

                 602     288     187
 
MslI CAynn'nnrTG

Cuts at:      0     115     265     500     847    1077 
   Size:        115     150     235     347     230

  Fragments arranged by size: 

                 347     235     230     150     115
 
MspA1I CmG'CkG

Cuts at:      0     278    1077 
   Size:        278     799
 
PfoI T'CCnGG_A

Cuts at:      0     754    1077 
   Size:        754     323
 
PmlI CAC'GTG

Cuts at:      0     359    1077 
   Size:        359     718
 
SfcI C'TryA_G

Cuts at:      0     819    1077 
   Size:        819     258
 
SspI AAT'ATT

Cuts at:      0     128     528     773    1077 
   Size:        128     400     245     304

  Fragments arranged by size: 

                 400     304     245     128
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     410    1077 
   Size:        410     667
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     600    1077 
   Size:        600     477
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     ApoI     BbsI     BclI     BmrI
     BpmI    BsaAI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
     ClaI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I
     MfeI     MscI     MslI   MspA1I     PfoI     PmlI     SfcI     SspI
    TaqII     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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