Restriction map for pF1KB7154
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaHI       BsgI        BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          337  GTCTAC               GT^[AC][GT]AC
AccIII        158  TCCGGA               T^CCGGA
AciI          366  CCGC                 CCGC(-3/-1)
AfaI          503  GTAC                 GT^AC
AocI         1033  CCTGAGG              CC^T[ACGT]AGG
Asp700I       573  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          43  GTGCTC               G[AT]GC[AT]^C
BclI          344  TGATCA               T^GATCA
BsaAI         719  TACGTG               [CT]AC^GT[GA]
BsaI          246  GGTCTC               GGTCTC(1/5)
BsaWI         158  TCCGGA               [AT]^CCGG[AT]
BseAI         158  TCCGGA               T^CCGGA
BsiHKAI        43  GTGCTC               G[AT]GC[AT]^C
BsmBI        1039  GAGACG               CGTCTC(1/5)
BspEI         158  TCCGGA               T^CCGGA
BstYI          77  GGATCT               [GA]^GATC[CT]
Bsu36I       1033  CCTGAGG              CC^T[ACGT]AGG
Eco81I       1033  CCTGAGG              CC^T[ACGT]AGG
EcoNI         972  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          344  TGATCA               T^GATCA
HapII         159  CCGG                 C^CGG
HincII        175  GTCAAC               GT[CT]^[GA]AC
HindII        175  GTCAAC               GT[CT]^[GA]AC
HpaII         159  CCGG                 C^CGG
MflI           77  GGATCT               [GA]^GATC[CT]
MroI          158  TCCGGA               T^CCGGA
MspI          159  CCGG                 C^CGG
NcoI          587  CCATGG               C^CATGG
PshAI         975  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         882  CAGCTG               CAG^CTG
RsaI          503  GTAC                 GT^AC
ScaI          502  AGTACT               AGT^ACT
SfaNI         384  GCATC                GCATC(5/9)
SfcI          560  CTGTAG               C^T[GA][CT]AG
SpeI          955  ACTAGT               A^CTAGT
XhoII          77  GGATCT               [GA]^GATC[CT]
XmnI          573  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          477  AAATTT               [GA]^AATT[CT]
              680  AAATTC              
AflIII        575  ACGTGT               A^C[GA][CT]GT
              720  ACGTGT              
ApaI          395  GGGCCC               GGGCC^C
              968  GGGCCC              
ApoI          477  AAATTT               [GA]^AATT[CT]
              680  AAATTC              
BanII         395  GGGCCC               G[GA]GC[CT]^C
              968  GGGCCC              
BbsI          112  GTCTTC               GAAGAC(2/6)
              724  GTCTTC              
BpuAI         112  GTCTTC               GAAGAC(2/6)
              724  GTCTTC              
BsaBI         312  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              345  GATCACCATC          
BseRI          37  GAGGAG               GAGGAG(10/8)
             1036  GAGGAG              
BsmAI        1039  GAGAC                GTCTC(1/5)
              247  GTCTC               
BsmI          541  GCATTC               GAATGC(1/-1)
              782  GCATTC              
BstXI         413  CCATGAGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              953  CCACTAGTTTGG        
DsaI           96  CCACGG               C^C[GA][CT]GG
              587  CCATGG              
MamI          312  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              345  GATCACCATC          
MspA1I        366  CCGCTG               C[AC]G^C[GT]G
              882  CAGCTG              
MwoI           45  GCTCCCTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              733  GCTGTGGTGGC         
PpuMI         119  GGGTCCT              [GA]G^G[AT]CC[CT]
              980  AGGTCCC             
TfiI          155  GATTC                G^A[AT]TC
              939  GATTC               
Tsp45I        103  GTCAC                ^GT[GC]AC
              551  GTGAC               
XcmI          350  CCATCATTGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              612  CCACTTCATTATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AluI        AlwI        AvaII       BalI        BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrDI       BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       StyI        
Tru9I       TseI        Tsp509I     TspRI       
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