Restriction map for pF1KB7144
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BsaAI       
BsaHI       BsaI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspMI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MfeI        MluI        
MluNI       MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        266  TCCGGA               T^CCGGA
AcsI           91  AAATTT               [GA]^AATT[CT]
AflIII        347  ACATGT               A^C[GA][CT]GT
AluI          608  AGCT                 AG^CT
ApoI           91  AAATTT               [GA]^AATT[CT]
BamHI         900  GGATCC               G^GATCC
BanII         308  GAGCCC               G[GA]GC[CT]^C
BciVI         556  GGATAC               GTATCC(6/5)
BlpI          604  GCTGAGC              GC^T[ACGT]AGC
BmyI          308  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I      604  GCTGAGC              GC^T[ACGT]AGC
BsaBI         633  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         266  TCCGGA               [AT]^CCGG[AT]
BseAI         266  TCCGGA               T^CCGGA
BseRI         403  CTCCTC               GAGGAG(10/8)
BsgI          158  GTGCAG               GTGCAG(16/14)
BsmI          570  GAATGC               GAATGC(1/-1)
Bsp1286I      308  GAGCCC               G[AGT]GC[ACT]^C
BspEI         266  TCCGGA               T^CCGGA
BspLU11I      347  ACATGT               A^CATGT
BsrBI         972  CCGCTC               CCGCTC(-3/-3)
BstYI         900  GGATCC               [GA]^GATC[CT]
CelII         604  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        419  GGGCC                G^G[ACGT]CC
HapII         267  CCGG                 C^CGG
HinfI         842  GAATC                G^A[ACGT]TC
HpaII         267  CCGG                 C^CGG
MaeII         373  ACGT                 A^CGT
MamI          633  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          900  GGATCC               [GA]^GATC[CT]
MroI          266  TCCGGA               T^CCGGA
MspI          267  CCGG                 C^CGG
NlaIV         900  GGATCC               GG[ACGT]^[ACGT]CC
NspI          347  ACATGT               [GA]CATG^[CT]
PflMI         422  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          746  CTGCAG               CTGCA^G
Sau96I        419  GGGCC                G^G[ACGT]CC
SfcI          746  CTGCAG               C^T[GA][CT]AG
TfiI          842  GAATC                G^A[AT]TC
Van91I        422  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         900  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         423  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              599  CAGTTGCTG           
BclI          218  TGATCA               T^GATCA
              632  TGATCA              
BsiYI         225  CCTCTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              422  CCAGAGACTGG         
BslI          225  CCTCTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              422  CCAGAGACTGG         
BsmFI         481  GGGAC                GGGAC(10/14)
              830  GTCCC               
BspHI         215  TCATGA               T^CATGA
              983  TCATGA              
BssKI         139  CCTGG                ^CC[ACGT]GG
              653  CCTGG               
BstNI         139  CCTGG                CC^[AT]GG
              653  CCTGG               
DsaI          800  CCATGG               C^C[GA][CT]GG
              954  CCATGG              
EcoRII        139  CCTGG                ^CC[AT]GG
              653  CCTGG               
FbaI          218  TGATCA               T^GATCA
              632  TGATCA              
HaeIII        296  GGCC                 GG^CC
              420  GGCC                
MseI          523  TTAA                 T^TAA
              782  TTAA                
MslI           81  CACTAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              210  CATTTTCATG          
MvaI          139  CCTGG                CC^[AT]GG
              653  CCTGG               
NcoI          800  CCATGG               C^CATGG
              954  CCATGG              
RcaI          215  TCATGA               T^CATGA
              983  TCATGA              
ScrFI         139  CCTGG                CC^[ACGT]GG
              653  CCTGG               
SfaNI         377  GCATC                GCATC(5/9)
              452  GCATC               
SmlI          406  CTCAAG               C^T[CT][GA]AG
              672  CTTGAG              
Tru9I         523  TTAA                 T^TAA
              782  TTAA                
Tsp509I        92  AATT                 ^AATT
              906  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AfaI        AlwI        BbsI        BbvI        
BpuAI       BsaJI       BsmAI       BsrI        BstF5I      
Cac8I       DdeI        DpnII       EcoT14I     FauI        
Fnu4HI      FokI        ItaI        MaeIII      MboI        
MboII       MnlI        MwoI        NdeII       NlaIII      
RsaI        Sau3AI      StyI        TseI        Tsp45I      
TspRI       
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