Restriction map for pF1KB7140
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BcnI        BfaI        
BfrI        BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cac8I       CelII       CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          803  GTAGAC               GT^[AC][GT]AC
AciI           39  GCGG                 CCGC(-3/-1)
AcsI          904  AAATTC               [GA]^AATT[CT]
AfaI           64  GTAC                 GT^AC
AocI          992  CCTTAGG              CC^T[ACGT]AGG
ApoI          904  AAATTC               [GA]^AATT[CT]
BamHI         873  GGATCC               G^GATCC
BanII         340  GGGCTC               G[GA]GC[CT]^C
BbsI          724  GTCTTC               GAAGAC(2/6)
BcgI          493  GCAACACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          534  GCCTCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          31  AGATCT               A^GATCT
BpuAI         724  GTCTTC               GAAGAC(2/6)
BsaJI         259  CCCTGG               C^C[ACGT][ACGT]GG
BseRI         159  CTCCTC               GAGGAG(10/8)
BsiYI         524  CCTTAAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          524  CCTTAAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrBI          37  GAGCGG               CCGCTC(-3/-3)
BsrFI         106  ACCGGC               [GA]^CCGG[CT]
BsrI          576  CCAGT                ACTGG(1/-1)
BstXI         314  CCATGTATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        992  CCTTAGG              CC^T[ACGT]AGG
Cfr10I        106  ACCGGC               [GA]^CCGG[CT]
Cfr13I        532  GGGCC                G^G[ACGT]CC
DraII         531  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          197  TGGCCG               [CT]^GGCC[GA]
Eco81I        992  CCTTAGG              CC^T[ACGT]AGG
EcoO109I      531  GGGGCCT              [GA]G^G[ACGT]CC[CT]
HapII         107  CCGG                 C^CGG
HpaII         107  CCGG                 C^CGG
HphI          297  GGTGA                GGTGA(8/7)
MspA1I         67  CAGCTG               C[AC]G^C[GT]G
MspI          107  CCGG                 C^CGG
NdeI          570  CATATG               CA^TATG
PleI          244  GACTC                GAGTC(4/5)
PvuII          67  CAGCTG               CAG^CTG
RsaI           64  GTAC                 GT^AC
Sau96I        532  GGGCC                G^G[ACGT]CC
SfcI          801  CTGTAG               C^T[GA][CT]AG
SspI          388  AATATT               AAT^ATT
Tsp509I       905  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          874  GATCC                GGATC(4/5)
              873  GGATC               
BanI           41  GGTGCC               G^G[CT][GA]CC
              255  GGCACC              
BbvI          264  GCAGC                GCAGC(8/12)
              853  GCAGC               
BmyI           42  GTGCCC               G[AGT]GC[ACT]^C
              340  GGGCTC              
BsaI          310  GAGACC               GGTCTC(1/5)
              423  GGTCTC              
Bsp1286I       42  GTGCCC               G[AGT]GC[ACT]^C
              340  GGGCTC              
BssKI         252  CCTGG                ^CC[ACGT]GG
              260  CCTGG               
BstF5I        111  CATCC                GGATG(2/0)
              150  CATCC               
BstNI         252  CCTGG                CC^[AT]GG
              260  CCTGG               
BstYI          31  AGATCT               [GA]^GATC[CT]
              873  GGATCC              
EcoRII        252  CCTGG                ^CC[AT]GG
              260  CCTGG               
Fnu4HI        264  GCAGC                GC^[ACGT]GC
              853  GCAGC               
FokI          111  CATCC                GGATG(9/13)
              150  CATCC               
HaeIII        198  GGCC                 GG^CC
              533  GGCC                
ItaI          264  GCAGC                GC^[ACGT]GC
              853  GCAGC               
MflI           31  AGATCT               [GA]^GATC[CT]
              873  GGATCC              
MseI          526  TTAA                 T^TAA
              880  TTAA                
MvaI          252  CCTGG                CC^[AT]GG
              260  CCTGG               
ScrFI         252  CCTGG                CC^[ACGT]GG
              260  CCTGG               
SmlI          364  CTCAAG               C^T[CT][GA]AG
              477  CTTGAG              
TfiI          359  GATTC                G^A[AT]TC
              646  GATTC               
Tru9I         526  TTAA                 T^TAA
              880  TTAA                
TseI          264  GCAGC                G^C[AT]GC
              853  GCAGC               
TspRI          11  CAGTG                CA[GC]TG[ACGT][ACGT]^
               92  CAGTG               
XhoII          31  AGATCT               [GA]^GATC[CT]
              873  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BsmAI       DdeI        DpnII       HinfI       
MboI        MboII       MnlI        MslI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      SfaNI       
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