Restriction map for pF1KB7119
 (Linear) (Six-base) MAPSORT of: KIBB7119.seq  Check: 6084  from: 1  to: 861



KIBB7119.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     774     861 
   Size:        774      87
 
AccI GT'mk_AC

Cuts at:      0     758     861 
   Size:        758     103
 
AflIII A'CryG_T

Cuts at:      0     536     560     861 
   Size:        536      24     301

  Fragments arranged by size: 

                 536     301      24
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      85     117     861 
   Size:         85      32     744

  Fragments arranged by size: 

                 744      85      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171     861 
   Size:        171     690
 
AvaI C'yCGr_G

Cuts at:      0     284     516     861 
   Size:        284     232     345

  Fragments arranged by size: 

                 345     284     232
 
BanI G'GyrC_C

Cuts at:      0     230     861 
   Size:        230     631
 
BanII G_rGCy'C

Cuts at:      0     680     740     861 
   Size:        680      60     121

  Fragments arranged by size: 

                 680     121      60
 
BbeI G_GCGC'C

Cuts at:      0     234     861 
   Size:        234     627
 
BbvCI CC'TCA_GC

Cuts at:      0     235     341     861 
   Size:        235     106     520

  Fragments arranged by size: 

                 520     235     106
 
BglI GCCn_nnn'nGGC

Cuts at:      0     280     861 
   Size:        280     581
 
BlpI GC'TnA_GC

Cuts at:      0     328     861 
   Size:        328     533
 
BmgBI CAC'GTC

Cuts at:      0     183     221     561     588     861 
   Size:        183      38     340      27     273

  Fragments arranged by size: 

                 340     273     183      38      27
 
BmtI G_CTAG'C

Cuts at:      0      16     861 
   Size:         16     845
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      96     861 
   Size:         96     765
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     514     670     861 
   Size:        142     372     156     191

  Fragments arranged by size: 

                 372     191     156     142
 
Bpu10I CC'TnA_GC

Cuts at:      0     235     341     861 
   Size:        235     106     520

  Fragments arranged by size: 

                 520     235     106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841     861 
   Size:        841      20
 
BsaBI GATnn'nnATC

Cuts at:      0     478     861 
   Size:        478     383
 
BsaHI Gr'CG_yC

Cuts at:      0     231     861 
   Size:        231     630
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145     244     274     861 
   Size:        115      30      99      30     587

  Fragments arranged by size: 

                 587     115      99      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     823     861 
   Size:        823      38
 
BseYI C'CCAG_C

Cuts at:      0     192     347     861 
   Size:        192     155     514

  Fragments arranged by size: 

                 514     192     155
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     861 
   Size:        820      41
 
BsiEI CG_ry'CG

Cuts at:      0     758     861 
   Size:        758     103
 
BsiHKAI G_wGCw'C

Cuts at:      0     680     740     861 
   Size:        680      60     121

  Fragments arranged by size: 

                 680     121      60
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     100     861 
   Size:        100     761
 
Bsp1286I G_dGCh'C

Cuts at:      0     680     740     861 
   Size:        680      60     121

  Fragments arranged by size: 

                 680     121      60
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     774     861 
   Size:        774      87
 
BsrFI r'CCGG_y

Cuts at:      0       8     766     861 
   Size:          8     758      95

  Fragments arranged by size: 

                 758      95       8
 
BssHII G'CGCG_C

Cuts at:      0     312     373     420     596     861 
   Size:        312      61      47     176     265

  Fragments arranged by size: 

                 312     265     176      61      47
 
BssSI C'ACGA_G

Cuts at:      0      88     673     861 
   Size:         88     585     188

  Fragments arranged by size: 

                 585     188      88
 
BstYI r'GATC_y

Cuts at:      0     473     861 
   Size:        473     388
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     249     861 
   Size:        249     612
 
BtsI GCAGTG_nn'

Cuts at:      0     300     374     861 
   Size:        300      74     487

  Fragments arranged by size: 

                 487     300      74
 
EaeI y'GGCC_r

Cuts at:      0     176     581     768     861 
   Size:        176     405     187      93

  Fragments arranged by size: 

                 405     187     176      93
 
EarI CTCTTCn'nnn_

Cuts at:      0     685     861 
   Size:        685     176
 
EcoICRI GAG'CTC

Cuts at:      0     678     738     861 
   Size:        678      60     123

  Fragments arranged by size: 

                 678     123      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     514     670     861 
   Size:        142     372     156     191

  Fragments arranged by size: 

                 372     191     156     142
 
HaeII r_GCGC'y

Cuts at:      0     234     489     861 
   Size:        234     255     372

  Fragments arranged by size: 

                 372     255     234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     244     276     861 
   Size:        244      32     585

  Fragments arranged by size: 

                 585     244      32
 
HincII GTy'rAC

Cuts at:      0     759     861 
   Size:        759     102
 
KasI G'GCGC_C

Cuts at:      0     230     861 
   Size:        230     631
 
MslI CAynn'nnrTG

Cuts at:      0     223     861 
   Size:        223     638
 
MspA1I CmG'CkG

Cuts at:      0     168     492     861 
   Size:        168     324     369

  Fragments arranged by size: 

                 369     324     168
 
NarI GG'CG_CC

Cuts at:      0     231     861 
   Size:        231     630
 
NheI G'CTAG_C

Cuts at:      0      12     861 
   Size:         12     849
 
NspI r_CATG'y

Cuts at:      0     540     861 
   Size:        540     321
 
PciI A'CATG_T

Cuts at:      0     536     861 
   Size:        536     325
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     171     861 
   Size:        120      51     690

  Fragments arranged by size: 

                 690     120      51
 
PfoI T'CCnGG_A

Cuts at:      0     119     143     861 
   Size:        119      24     718

  Fragments arranged by size: 

                 718     119      24
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555     861 
   Size:        523      32     306

  Fragments arranged by size: 

                 523     306      32
 
PvuII CAG'CTG

Cuts at:      0     168     492     861 
   Size:        168     324     369

  Fragments arranged by size: 

                 369     324     168
 
SacI G_AGCT'C

Cuts at:      0     680     740     861 
   Size:        680      60     121

  Fragments arranged by size: 

                 680     121      60
 
SalI G'TCGA_C

Cuts at:      0     757     861 
   Size:        757     104
 
SapI GCTCTTCn'nnn_

Cuts at:      0     685     861 
   Size:        685     176
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     280     861 
   Size:        280     581
 
SfoI GGC'GCC

Cuts at:      0     232     861 
   Size:        232     629
 
SmaI CCC'GGG

Cuts at:      0     286     861 
   Size:        286     575
 
SmlI C'TyrA_G

Cuts at:      0     856     861 
   Size:        856       5
 
StyI C'CwwG_G

Cuts at:      0     269     776     861 
   Size:        269     507      85

  Fragments arranged by size: 

                 507     269      85
 
TatI w'GTAC_w

Cuts at:      0     392     861 
   Size:        392     469
 
XmaI C'CCGG_G

Cuts at:      0     284     861 
   Size:        284     577
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AloI    AlwNI     AvaI     BanI    BanII
     BbeI    BbvCI     BglI     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BssHII    BssSI
    BstYI    BtgZI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    HaeII
    Hin4I   HincII     KasI     MslI   MspA1I     NarI     NheI     NspI
     PciI    PflMI     PfoI     PpiI    PvuII     SacI     SalI     SapI
     SfiI     SfoI     SmaI     SmlI     StyI     TatI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII Bme1580I     BmrI     BsaI    BsaAI    BsaWI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII    SanDI
     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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