Restriction map for pF1KB7113
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HinfI       HpaI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PleI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          103  GTATAC               GT^[AC][GT]AC
AcyI          577  GGCGCC               G[GA]^CG[CT]C
ApaI          230  GGGCCC               GGGCC^C
Asp700I       552  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         613  GAGCTC               G[AT]GC[AT]^C
AvaI          668  CTCGAG               C^[CT]CG[GA]G
BamHI         753  GGATCC               G^GATCC
BbeI          577  GGCGCC               GGCGC^C
BbrPI         280  CACGTG               CAC^GTG
BcgI          670  CGAGGGGGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          493  GCCAACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           16  CTCCAG               CTGGAG(16/14)
BsaHI         577  GGCGCC               G[GA]^CG[CT]C
BseRI         295  GAGGAG               GAGGAG(10/8)
BsiHKAI       613  GAGCTC               G[AT]GC[AT]^C
BsmFI         329  GGGAC                GGGAC(10/14)
BsoBI         668  CTCGAG               C^[CT]CG[GA]G
BsrDI         531  CATTGC               GCAATG(2/0)
BsrFI         308  GCCGGC               [GA]^CCGG[CT]
Bst1107I      103  GTATAC               GTA^TAC
BstXI         512  CCAACATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         753  GGATCC               [GA]^GATC[CT]
BstZ17I       103  GTATAC               GTA^TAC
Cfr10I        308  GCCGGC               [GA]^CCGG[CT]
DdeI           28  CTGAG                C^T[ACGT]AG
DpnII         754  GATC                 ^GATC
DsaI           77  CCGTGG               C^C[GA][CT]GG
EarI          430  CTCTTC               CTCTTC(1/4)
EcoNI         651  CCTGCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          300  GCGGG                CCCGC(4/6)
Hin1I         577  GGCGCC               G[GA]^CG[CT]C
KasI          577  GGCGCC               G^GCGCC
Ksp632I       430  CTCTTC               CTCTTC(1/4)
MaeIII        250  GTCAC                ^GT[ACGT]AC
MboI          754  GATC                 ^GATC
MflI          753  GGATCC               [GA]^GATC[CT]
MspA1I        717  CCGCTG               C[AC]G^C[GT]G
NaeI          308  GCCGGC               GCC^GGC
NarI          577  GGCGCC               GG^CGCC
NdeII         754  GATC                 ^GATC
NgoMIV        308  GCCGGC               G^CCGGC
NspI          253  ACATGC               [GA]CATG^[CT]
PaeR7I        668  CTCGAG               C^TCGAG
PflMI         726  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         280  CACGTG               CAC^GTG
PmlI          280  CACGTG               CAC^GTG
PpuMI         638  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI          613  GAGCTC               GAGCT^C
Sau3AI        754  GATC                 ^GATC
ScaI           44  AGTACT               AGT^ACT
SfoI          577  GGCGCC               GGC^GCC
SmlI          668  CTCGAG               C^T[CT][GA]AG
TaqI          669  TCGA                 T^CGA
Tsp45I        250  GTCAC                ^GT[GC]AC
TthHB8I       669  TCGA                 T^CGA
Van91I        726  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          668  CTCGAG               C^TCGAG
XhoII         753  GGATCC               [GA]^GATC[CT]
XmnI          552  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         124  CGCG                 CG^CG
              476  CGCG                
AlwI          754  GATCC                GGATC(4/5)
              753  GGATC               
BanI          311  GGCACC               G^G[CT][GA]CC
              577  GGCGCC              
BanII         230  GGGCCC               G[GA]GC[CT]^C
              613  GAGCTC              
BbsI            9  GAAGAC               GAAGAC(2/6)
               88  GTCTTC              
BcnI           93  CCGGG                CC^[GC]GG
              172  CCCGG               
BmyI          230  GGGCCC               G[AGT]GC[ACT]^C
              613  GAGCTC              
BpuAI           9  GAAGAC               GAAGAC(2/6)
               88  GTCTTC              
BsaAI         106  TACGTG               [CT]AC^GT[GA]
              280  CACGTG              
BsaJI          77  CCGTGG               C^C[ACGT][ACGT]GG
              762  CCCAGG              
Bsp1286I      230  GGGCCC               G[AGT]GC[ACT]^C
              613  GAGCTC              
BspMI         440  ACCTGC               ACCTGC(4/8)
              712  ACCTGC              
BsrBI          30  GAGCGG               CCGCTC(-3/-3)
              298  GAGCGG              
BsrI            5  ACTGG                ACTGG(1/-1)
              333  CCAGT               
BstUI         124  CGCG                 CG^CG
              476  CGCG                
HaeII         305  AGCGCC               [GA]GCGC^[CT]
              577  GGCGCC              
HphI          560  TCACC                GGTGA(8/7)
              602  TCACC               
MvnI          124  CGCG                 CG^CG
              476  CGCG                
NciI           93  CCGGG                CC^[GC]GG
              172  CCCGG               
SfaNI          65  GCATC                GCATC(5/9)
              593  GCATC               
StuI          375  AGGCCT               AGG^CCT
              648  AGGCCT              
TspRI         334  CAGTG                CA[GC]TG[ACGT][ACGT]^
              487  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BsgI        BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DraII       
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MaeII       MboII       MnlI        MspI        MvaI        
MwoI        NlaIII      NlaIV       PstI        RsaI        
Sau96I      ScrFI       SfcI        TseI        
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