Restriction map for
pF1KE1069
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AluI 244 AGCT AG^CT
AlwI 153 GGATC GGATC(4/5)
BbeI 421 GGCGCC GGCGC^C
BlpI 453 GCTCAGC GC^T[ACGT]AGC
BmyI 202 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 453 GCTCAGC GC^T[ACGT]AGC
BseRI 85 CTCCTC GAGGAG(10/8)
BsgI 13 GTGCAG GTGCAG(16/14)
BsmAI 387 GTCTC GTCTC(1/5)
BsmFI 228 GTCCC GGGAC(10/14)
BsmI 55 GCATTC GAATGC(1/-1)
Bsp1286I 202 GGGCAC G[AGT]GC[ACT]^C
BspMI 65 ACCTGC ACCTGC(4/8)
BstYI 153 GGATCT [GA]^GATC[CT]
CelII 453 GCTCAGC GC^T[ACGT]AGC
CfoI 422 GCGC GCG^C
DdeI 454 CTCAG C^T[ACGT]AG
DpnII 154 GATC ^GATC
DraIII 7 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 87 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 92 CCAAGG C^C[AT][AT]GG
HaeII 421 GGCGCC [GA]GCGC^[CT]
HgaI 256 GCGTC GACGC(5/10)
HhaI 422 GCGC GCG^C
HinP1I 422 GCGC G^CGC
HincII 508 GTCAAC GT[CT]^[GA]AC
HindII 508 GTCAAC GT[CT]^[GA]AC
HinfI 123 GAATC G^A[ACGT]TC
HphI 89 TCACC GGTGA(8/7)
KasI 421 GGCGCC G^GCGCC
MaeII 413 ACGT A^CGT
MaeIII 502 GTGAC ^GT[ACGT]AC
MboI 154 GATC ^GATC
MboII 525 GAAGA GAAGA(8/7)
MflI 153 GGATCT [GA]^GATC[CT]
MwoI 322 GCCGTCGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 421 GGCGCC GG^CGCC
NdeII 154 GATC ^GATC
NspI 398 GCATGC [GA]CATG^[CT]
Sau3AI 154 GATC ^GATC
SfaNI 479 GCATC GCATC(5/9)
SfoI 421 GGCGCC GGC^GCC
SmaI 236 CCCGGG CCC^GGG
SmlI 246 CTCAAG C^T[CT][GA]AG
SphI 398 GCATGC GCATG^C
StyI 92 CCAAGG C^C[AT][AT]GG
TaqI 390 TCGA T^CGA
TfiI 123 GAATC G^A[AT]TC
Tsp45I 502 GTGAC ^GT[GC]AC
TspRI 218 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 390 TCGA T^CGA
XhoII 153 GGATCT [GA]^GATC[CT]
XmaCI 236 CCCGGG C^CCGGG
XmaI 236 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcyI 255 GGCGTC G[GA]^CG[CT]C
421 GGCGCC
AvaI 141 CTCGGG C^[CT]CG[GA]G
236 CCCGGG
AvaII 63 GGACC G^G[AT]CC
227 GGTCC
BanI 203 GGCACC G^G[CT][GA]CC
421 GGCGCC
BbvI 395 GCAGC GCAGC(8/12)
450 GCTGC
BpmI 157 CTGGAG CTGGAG(16/14)
208 CTGGAG
BsaHI 255 GGCGTC G[GA]^CG[CT]C
421 GGCGCC
BsoBI 141 CTCGGG C^[CT]CG[GA]G
236 CCCGGG
EaeI 320 TGGCCG [CT]^GGCC[GA]
497 TGGCCG
Hin1I 255 GGCGTC G[GA]^CG[CT]C
421 GGCGCC
MslI 468 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
486 CATTATCATG
TseI 395 GCAGC G^C[AT]GC
450 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BcnI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MnlI
MspI MvaI NciI NlaIII NlaIV
Sau96I ScrFI