Restriction map for pF1KB7043
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvrII       BalI        
BamHI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsgI        BsmI        BspDI       BspHI       
BspLU11I    BspMI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EarI        
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MaeIII      
MamI        MfeI        MluI        MluNI       MscI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        Tsp45I      Tsp509I     
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        678  TCCGGA               T^CCGGA
AhdI          297  GACGCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           79  CCTGAGG              CC^T[ACGT]AGG
AspEI         297  GACGCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         860  TGCGCA               TGC^GCA
BbrPI         469  CACGTG               CAC^GTG
BbvCI         773  CCTCAGC              CCTCAGC(-5/-2)
BfaI          452  CTAG                 C^TAG
BsaAI         469  CACGTG               [CT]AC^GT[GA]
BsaI          979  GAGACC               GGTCTC(1/5)
BsaWI         678  TCCGGA               [AT]^CCGG[AT]
BseAI         678  TCCGGA               T^CCGGA
BsiWI         269  CGTACG               C^GTACG
BsmBI          91  GAGACG               CGTCTC(1/5)
Bsp1407I     1399  TGTACA               T^GTACA
BspEI         678  TCCGGA               T^CCGGA
BsrDI        1329  CATTGC               GCAATG(2/0)
BsrGI        1399  TGTACA               T^GTACA
BssHII         69  GCGCGC               G^CGCGC
BssSI         651  CACGAG               CACGAG(-5/-1)
BstAPI        846  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         956  AGATCC               [GA]^GATC[CT]
Bsu36I         79  CCTGAGG              CC^T[ACGT]AGG
Eam1105I      297  GACGCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         79  CCTGAGG              CC^T[ACGT]AGG
FspI          860  TGCGCA               TGC^GCA
KspI          418  CCGCGG               CCGC^GG
MaeI          452  CTAG                 C^TAG
MflI          956  AGATCC               [GA]^GATC[CT]
MroI          678  TCCGGA               T^CCGGA
NaeI         1035  GCCGGC               GCC^GGC
NgoMIV       1035  GCCGGC               G^CCGGC
PmaCI         469  CACGTG               CAC^GTG
PmlI          469  CACGTG               CAC^GTG
PvuII         847  CAGCTG               CAG^CTG
SacII         418  CCGCGG               CCGC^GG
ScaI          809  AGTACT               AGT^ACT
SgrAI        1034  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        1399  TGTACA               T^GTACA
XcmI          499  CCAGGGGGCGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         956  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AlwNI         826  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              844  CAGCAGCTG           
ApaI          695  GGGCCC               GGGCC^C
             1261  GGGCCC              
AspHI         654  GAGCAC               G[AT]GC[AT]^C
             1153  GTGCTC              
BbeI          276  GGCGCC               GGCGC^C
              285  GGCGCC              
BseRI         714  CTCCTC               GAGGAG(10/8)
              682  GAGGAG              
BsiEI         688  CGGCCG               CG[GA][CT]^CG
              730  CGGCCG              
BsiHKAI       654  GAGCAC               G[AT]GC[AT]^C
             1153  GTGCTC              
BsrFI         732  GCCGGT               [GA]^CCGG[CT]
             1035  GCCGGC              
BsrI           18  CCAGT                ACTGG(1/-1)
             1209  CCAGT               
Cfr10I        732  GCCGGT               [GA]^CCGG[CT]
             1035  GCCGGC              
EagI          688  CGGCCG               C^GGCCG
              730  CGGCCG              
EclXI         688  CGGCCG               C^GGCCG
              730  CGGCCG              
Eco52I        688  CGGCCG               C^GGCCG
              730  CGGCCG              
EcoT14I      1124  CCAAGG               C^C[AT][AT]GG
             1257  CCAAGG              
KasI          276  GGCGCC               G^GCGCC
              285  GGCGCC              
MseI         1160  TTAA                 T^TAA
             1286  TTAA                
MslI           45  CATGCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1242  CACCAGGATG          
NarI          276  GGCGCC               GG^CGCC
              285  GGCGCC              
PflMI         843  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1097  CCAAATGCTGG         
SfoI          276  GGCGCC               GGC^GCC
              285  GGCGCC              
SmaI          139  CCCGGG               CCC^GGG
              337  CCCGGG              
SphI          245  GCATGC               GCATG^C
              944  GCATGC              
StyI         1124  CCAAGG               C^C[AT][AT]GG
             1257  CCAAGG              
TaqI          917  TCGA                 T^CGA
             1280  TCGA                
TfiI         1274  GATTC                G^A[AT]TC
             1310  GATTC               
Tru9I        1160  TTAA                 T^TAA
             1286  TTAA                
TthHB8I       917  TCGA                 T^CGA
             1280  TCGA                
Van91I        843  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1097  CCAAATGCTGG         
XmaCI         139  CCCGGG               C^CCGGG
              337  CCCGGG              
XmaI          139  CCCGGG               C^CCGGG
              337  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TseI        TspRI       
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