Restriction map for pF1KB7035
 (Linear) (Six-base) MAPSORT of: KIBB7035.seq  Check: 4037  from: 1  to: 1278



KIBB7035.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AccI GT'mk_AC

Cuts at:      0     632    1278 
   Size:        632     646
 
Acc65I G'GTAC_C

Cuts at:      0     231    1278 
   Size:        231    1047
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     920    1278 
   Size:        920     358
 
AhdI GACnn_n'nnGTC

Cuts at:      0     105     217    1278 
   Size:        105     112    1061

  Fragments arranged by size: 

                1061     112     105
 
AvaI C'yCGr_G

Cuts at:      0    1062    1278 
   Size:       1062     216
 
BamHI G'GATC_C

Cuts at:      0      73    1278 
   Size:         73    1205
 
BanI G'GyrC_C

Cuts at:      0     231     751    1278 
   Size:        231     520     527

  Fragments arranged by size: 

                 527     520     231
 
BanII G_rGCy'C

Cuts at:      0     709    1278 
   Size:        709     569
 
BbeI G_GCGC'C

Cuts at:      0     755    1278 
   Size:        755     523
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     406     651     908    1278 
   Size:        406     245     257     370

  Fragments arranged by size: 

                 406     370     257     245
 
Bme1580I G_kGCm'C

Cuts at:      0      86    1063    1178    1278 
   Size:         86     977     115     100

  Fragments arranged by size: 

                 977     115     100      86
 
BmgBI CAC'GTC

Cuts at:      0    1082    1278 
   Size:       1082     196
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     375    1278 
   Size:        375     903
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     654    1278 
   Size:        654     624
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     204    1182    1278 
   Size:        204     978      96

  Fragments arranged by size: 

                 978     204      96
 
BsaHI Gr'CG_yC

Cuts at:      0     752     921    1278 
   Size:        752     169     357

  Fragments arranged by size: 

                 752     357     169
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     280     310    1255    1278 
   Size:        280      30     945      23

  Fragments arranged by size: 

                 945     280      30      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69     615     808    1276    1278 
   Size:         69     546     193     468       2

  Fragments arranged by size: 

                 546     468     193      69       2
 
BseYI C'CCAG_C

Cuts at:      0     224    1278 
   Size:        224    1054
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     391     476    1278 
   Size:        181     210      85     802

  Fragments arranged by size: 

                 802     210     181      85
 
BsiHKAI G_wGCw'C

Cuts at:      0     649     709    1276    1278 
   Size:        649      60     567       2

  Fragments arranged by size: 

                 649     567      60       2
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     649     709    1063    1178    1276    1278 
   Size:         86     563      60     354     115      98       2

  Fragments arranged by size: 

                 563     354     115      98      86      60       2
 
BsrDI GCAATG_nn'

Cuts at:      0    1016    1278 
   Size:       1016     262
 
BsrFI r'CCGG_y

Cuts at:      0     228     917    1278 
   Size:        228     689     361

  Fragments arranged by size: 

                 689     361     228
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     748    1278 
   Size:        748     530
 
BstYI r'GATC_y

Cuts at:      0      73    1278 
   Size:         73    1205
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     898    1278 
   Size:        898     380
 
BtsI GCAGTG_nn'

Cuts at:      0     438     742    1278 
   Size:        438     304     536

  Fragments arranged by size: 

                 536     438     304
 
DraI TTT'AAA

Cuts at:      0     188    1278 
   Size:        188    1090
 
EaeI y'GGCC_r

Cuts at:      0     119    1278 
   Size:        119    1159
 
EarI CTCTTCn'nnn_

Cuts at:      0     202     475     529     563     637     785    1278 
   Size:        202     273      54      34      74     148     493

  Fragments arranged by size: 

                 493     273     202     148      74      54      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     508     833    1278 
   Size:        508     325     445

  Fragments arranged by size: 

                 508     445     325
 
EcoICRI GAG'CTC

Cuts at:      0     707    1278 
   Size:        707     571
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     654     920    1278 
   Size:        654     266     358

  Fragments arranged by size: 

                 654     358     266
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     130    1278 
   Size:        130    1148
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1088    1278 
   Size:       1088     190
 
HaeII r_GCGC'y

Cuts at:      0     247     755    1278 
   Size:        247     508     523

  Fragments arranged by size: 

                 523     508     247
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     203     235     860     892    1005    1037 
   Size:         91      32      80      32     625      32     113      32

Cuts at:   1037    1278 
   Size:        241

  Fragments arranged by size: 

                 625     241     113      91      80      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     954    1278 
   Size:        954     324
 
HindIII A'AGCT_T

Cuts at:      0     265    1278 
   Size:        265    1013
 
KasI G'GCGC_C

Cuts at:      0     751    1278 
   Size:        751     527
 
KpnI G_GTAC'C

Cuts at:      0     235    1278 
   Size:        235    1043
 
MslI CAynn'nnrTG

Cuts at:      0    1198    1255    1278 
   Size:       1198      57      23

  Fragments arranged by size: 

                1198      57      23
 
MspA1I CmG'CkG

Cuts at:      0     684    1278 
   Size:        684     594
 
NaeI GCC'GGC

Cuts at:      0     919    1278 
   Size:        919     359
 
NarI GG'CG_CC

Cuts at:      0     752    1278 
   Size:        752     526
 
NgoMIV G'CCGG_C

Cuts at:      0     917    1278 
   Size:        917     361
 
NspI r_CATG'y

Cuts at:      0     195     658    1134    1278 
   Size:        195     463     476     144

  Fragments arranged by size: 

                 476     463     195     144
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     599     631    1278 
   Size:        599      32     647

  Fragments arranged by size: 

                 647     599      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1088    1278 
   Size:       1088     190
 
PshAI GACnn'nnGTC

Cuts at:      0     508    1194    1278 
   Size:        508     686      84

  Fragments arranged by size: 

                 686     508      84
 
PstI C_TGCA'G

Cuts at:      0     887    1278 
   Size:        887     391
 
SacI G_AGCT'C

Cuts at:      0     709    1278 
   Size:        709     569
 
SapI GCTCTTCn'nnn_

Cuts at:      0     475     637    1278 
   Size:        475     162     641

  Fragments arranged by size: 

                 641     475     162
 
SfcI C'TryA_G

Cuts at:      0     883    1278 
   Size:        883     395
 
SfoI GGC'GCC

Cuts at:      0     753    1278 
   Size:        753     525
 
SgrAI Cr'CCGG_yG

Cuts at:      0     917    1278 
   Size:        917     361
 
SphI G_CATG'C

Cuts at:      0    1134    1278 
   Size:       1134     144
 
StyI C'CwwG_G

Cuts at:      0     236     422     485    1278 
   Size:        236     186      63     793

  Fragments arranged by size: 

                 793     236     186      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     238    1259    1278 
   Size:        238    1021      19

  Fragments arranged by size: 

                1021     238      19
 
TatI w'GTAC_w

Cuts at:      0    1205    1278 
   Size:       1205      73
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     714     746    1085    1117    1255    1278 
   Size:        714      32     339      32     138      23

  Fragments arranged by size: 

                 714     339     138      32      32      23
 
XmnI GAAnn'nnTTC

Cuts at:      0     973    1278 
   Size:        973     305
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AhdI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI Bme1580I    BmgBI     BplI     BpmI     BsaI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrFI
    BstXI    BstYI    BtgZI     BtsI     DraI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PpiI    PpuMI    PshAI     PstI     SacI     SapI     SfcI     SfoI
    SgrAI     SphI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII     DrdI     EagI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI     HpaI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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