Restriction map for pF1KB5656
 (Linear) (Six-base) MAPSORT of: KIBB5656.seq  Check: 527  from: 1  to: 1041



KIBB5656.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     858    1041 
   Size:        858     183
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     519    1041 
   Size:        166     353     522

  Fragments arranged by size: 

                 522     353     166
 
AfeI AGC'GCT

Cuts at:      0     451    1041 
   Size:        451     590
 
AhdI GACnn_n'nnGTC

Cuts at:      0     379    1041 
   Size:        379     662
 
AleI CACnn'nnGTG

Cuts at:      0     787    1041 
   Size:        787     254
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     916    1041 
   Size:        916     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0     852     873    1041 
   Size:        852      21     168

  Fragments arranged by size: 

                 852     168      21
 
ApoI r'AATT_y

Cuts at:      0     148     670    1041 
   Size:        148     522     371

  Fragments arranged by size: 

                 522     371     148
 
AvaI C'yCGr_G

Cuts at:      0     692    1041 
   Size:        692     349
 
BanI G'GyrC_C

Cuts at:      0     121    1041 
   Size:        121     920
 
BanII G_rGCy'C

Cuts at:      0     707    1041 
   Size:        707     334
 
BbeI G_GCGC'C

Cuts at:      0     125    1041 
   Size:        125     916
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      56     185     525    1041 
   Size:         56     129     340     516

  Fragments arranged by size: 

                 516     340     129      56
 
BclI T'GATC_A

Cuts at:      0     606    1041 
   Size:        606     435
 
BglI GCCn_nnn'nGGC

Cuts at:      0     595    1041 
   Size:        595     446
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      95    1041 
   Size:         95     946
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     180    1041 
   Size:        180     861
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     874    1041 
   Size:        820      54     167

  Fragments arranged by size: 

                 820     167      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152    1041 
   Size:        152     889
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     366     850    1041 
   Size:        366     484     191

  Fragments arranged by size: 

                 484     366     191
 
BsaAI yAC'GTr

Cuts at:      0     768    1041 
   Size:        768     273
 
BsaHI Gr'CG_yC

Cuts at:      0     122    1041 
   Size:        122     919
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     266     754    1041 
   Size:        266     488     287

  Fragments arranged by size: 

                 488     287     266
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     943    1041 
   Size:        943      98
 
BsiEI CG_ry'CG

Cuts at:      0    1038    1041 
   Size:       1038       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     707    1041 
   Size:        707     334
 
BsmI GAATG_Cn'

Cuts at:      0     753    1041 
   Size:        753     288
 
Bsp1286I G_dGCh'C

Cuts at:      0     707    1041 
   Size:        707     334
 
BspHI T'CATG_A

Cuts at:      0     338     603     609    1041 
   Size:        338     265       6     432

  Fragments arranged by size: 

                 432     338     265       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     858    1041 
   Size:        858     183
 
BsrBI CCG'CTC

Cuts at:      0     822    1041 
   Size:        822     219
 
BsrDI GCAATG_nn'

Cuts at:      0     219     306     420    1041 
   Size:        219      87     114     621

  Fragments arranged by size: 

                 621     219     114      87
 
BstYI r'GATC_y

Cuts at:      0     159    1041 
   Size:        159     882
 
Bsu36I CC'TnA_GG

Cuts at:      0     437    1041 
   Size:        437     604
 
BtsI GCAGTG_nn'

Cuts at:      0     794    1041 
   Size:        794     247
 
EarI CTCTTCn'nnn_

Cuts at:      0     293    1041 
   Size:        293     748
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     697    1041 
   Size:        697     344
 
EcoICRI GAG'CTC

Cuts at:      0     705    1041 
   Size:        705     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     519     820     874    1041 
   Size:        166     353     301      54     167

  Fragments arranged by size: 

                 353     301     167     166      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     865    1041 
   Size:        865     176
 
EcoRI G'AATT_C

Cuts at:      0     670    1041 
   Size:        670     371
 
FspI TGC'GCA

Cuts at:      0     781    1041 
   Size:        781     260
 
FspAI rTGC'GCAy

Cuts at:      0     781    1041 
   Size:        781     260
 
HaeII r_GCGC'y

Cuts at:      0     125     453    1041 
   Size:        125     328     588

  Fragments arranged by size: 

                 588     328     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      57      89     365     397    1041 
   Size:         57      32     276      32     644

  Fragments arranged by size: 

                 644     276      57      32      32
 
KasI G'GCGC_C

Cuts at:      0     121    1041 
   Size:        121     920
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     749    1041 
   Size:        749     292
 
MslI CAynn'nnrTG

Cuts at:      0     230     403     608     787    1041 
   Size:        230     173     205     179     254

  Fragments arranged by size: 

                 254     230     205     179     173
 
NarI GG'CG_CC

Cuts at:      0     122    1041 
   Size:        122     919
 
NspI r_CATG'y

Cuts at:      0     780     897    1041 
   Size:        780     117     144

  Fragments arranged by size: 

                 780     144     117
 
PfoI T'CCnGG_A

Cuts at:      0     815    1041 
   Size:        815     226
 
PmlI CAC'GTG

Cuts at:      0     768    1041 
   Size:        768     273
 
PvuI CG_AT'CG

Cuts at:      0    1038    1041 
   Size:       1038       3
 
SacI G_AGCT'C

Cuts at:      0     707    1041 
   Size:        707     334
 
SfcI C'TryA_G

Cuts at:      0     923    1007    1041 
   Size:        923      84      34

  Fragments arranged by size: 

                 923      84      34
 
SfoI GGC'GCC

Cuts at:      0     123    1041 
   Size:        123     918
 
SmlI C'TyrA_G

Cuts at:      0     167    1041 
   Size:        167     874
 
SphI G_CATG'C

Cuts at:      0     780     897    1041 
   Size:        780     117     144

  Fragments arranged by size: 

                 780     144     117
 
StuI AGG'CCT

Cuts at:      0     523    1041 
   Size:        523     518
 
StyI C'CwwG_G

Cuts at:      0      59    1041 
   Size:         59     982
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     113     321    1041 
   Size:        113     208     720

  Fragments arranged by size: 

                 720     208     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     126     158    1041 
   Size:        126      32     883

  Fragments arranged by size: 

                 883     126      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     359    1041 
   Size:        359     682
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI     AleI     AlfI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI     BclI     BglI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BseRI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BstYI   Bsu36I     BtsI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI     FspI    FspAI    HaeII    Hin4I     KasI     MmeI     MslI
     NarI     NspI     PfoI     PmlI     PvuI     SacI     SfcI     SfoI
     SmlI     SphI     StuI     StyI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI   Bpu10I    BsaBI    BsaWI    BsaXI    BseYI    BsiWI    BsmBI
    BspEI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI EcoO109I    EcoRV     FalI     FseI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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