################################## # NO CUT ENZYMES ( 223 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AciI 109 GCGG CCGC(-3/-1) AhdI 112 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 112 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 111 GGACC G^G[AT]CC BbvI 54 GCAGC GCAGC(8/12) BsaJI 35 CCCAGG C^C[ACGT][ACGT]GG BsgI 52 GTGCAG GTGCAG(16/14) BstBI 96 TTCGAA TT^CGAA Cac8I 16 GCAAGC GC[ACGT]^[ACGT]GC Cfr13I 111 GGACC G^G[ACGT]CC Eam1105I 112 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Fnu4HI 54 GCAGC GC^[ACGT]GC ItaI 54 GCAGC GC^[ACGT]GC MaeIII 146 GTGAC ^GT[ACGT]AC MseI 61 TTAA T^TAA MspA1I 107 CAGCGG C[AC]G^C[GT]G NspI 135 ACATGC [GA]CATG^[CT] NspV 96 TTCGAA TT^CGAA Sau96I 111 GGACC G^G[ACGT]CC SfuI 96 TTCGAA TT^CGAA TaqI 97 TCGA T^CGA Tru9I 61 TTAA T^TAA TseI 54 GCAGC G^C[AT]GC Tsp45I 146 GTGAC ^GT[GC]AC TthHB8I 97 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BsiYI 35 CCCAGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 183 CCCTTTCAAGG BslI 35 CCCAGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 183 CCCTTTCAAGG BssKI 36 CCAGG ^CC[ACGT]GG 140 CCAGG BstNI 36 CCAGG CC^[AT]GG 140 CCAGG EcoRII 36 CCAGG ^CC[AT]GG 140 CCAGG MvaI 36 CCAGG CC^[AT]GG 140 CCAGG NlaIII 117 CATG CATG^ 136 CATG ScrFI 36 CCAGG CC^[ACGT]GG 140 CCAGG SfaNI 103 GCATC GCATC(5/9) 206 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### MnlI