(Circular) (Six-base) MAPSORT of: pF1KB5643.seq Check: 561 from: 1 to: 3329 pF1KB5643 3329 bp With 182 enzymes: SgfI * June 20, 2008 10:25 .. AccI GT'mk_AC Cuts at: 367 367 Size: 3329 Acc65I G'GTAC_C Cuts at: 345 345 Size: 3329 AclI AA'CG_TT Cuts at: 3169 3169 Size: 3329 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 266 1154 1586 2333 2601 266 Size: 888 432 747 268 994 Fragments arranged by size: 994 888 747 432 268 AflIII A'CryG_T Cuts at: 681 1785 2762 681 Size: 1104 977 1248 Fragments arranged by size: 1248 1104 977 AgeI A'CCGG_T Cuts at: 1717 1717 Size: 3329 AhdI GACnn_n'nnGTC Cuts at: 225 225 Size: 3329 AlwNI CAG_nnn'CTG Cuts at: 634 2201 634 Size: 1567 1762 ApaLI G'TGCA_C Cuts at: 2099 2099 Size: 3329 ApoI r'AATT_y Cuts at: 333 2682 2938 333 Size: 2349 256 724 Fragments arranged by size: 2349 724 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3329 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3329 AvaI C'yCGr_G Cuts at: 349 349 Size: 3329 BamHI G'GATC_C Cuts at: 354 354 Size: 3329 BanI G'GyrC_C Cuts at: 345 759 1007 1042 345 Size: 414 248 35 2632 Fragments arranged by size: 2632 414 248 35 BanII G_rGCy'C Cuts at: 343 1373 343 Size: 1030 2299 BbeI G_GCGC'C Cuts at: 763 1011 763 Size: 248 3081 BciVI GTATCCnnnnn_n' Cuts at: 1227 1988 3329 1227 Size: 761 1341 1227 Fragments arranged by size: 1341 1227 761 BclI T'GATC_A Cuts at: 849 849 Size: 3329 BglII A'GATC_T Cuts at: 844 2881 844 Size: 2037 1292 BlpI GC'TnA_GC Cuts at: 441 441 Size: 3329 Bme1580I G_kGCm'C Cuts at: 954 1047 2103 954 Size: 93 1056 2180 Fragments arranged by size: 2180 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 541 622 957 3101 541 Size: 81 335 2144 769 Fragments arranged by size: 2144 769 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1696 1696 Size: 3329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 505 1384 1876 2174 505 Size: 879 492 298 1660 Fragments arranged by size: 1660 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3033 3033 Size: 3329 BsaAI yAC'GTr Cuts at: 682 682 Size: 3329 BsaBI GATnn'nnATC Cuts at: 6 843 2880 6 Size: 837 2037 455 Fragments arranged by size: 2037 837 455 BsaHI Gr'CG_yC Cuts at: 760 1008 1710 3206 760 Size: 248 702 1496 883 Fragments arranged by size: 1496 883 702 248 BsaWI w'CCGG_w Cuts at: 518 1039 1717 1991 2138 2549 518 Size: 521 678 274 147 411 1298 Fragments arranged by size: 1298 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 754 1512 2089 2585 754 Size: 758 577 496 1498 Fragments arranged by size: 1498 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 181 181 Size: 3329 BsiEI CG_ry'CG Cuts at: 104 871 917 2125 104 Size: 767 46 1208 1308 Fragments arranged by size: 1308 1208 767 46 BsiHKAI G_wGCw'C Cuts at: 343 1121 1311 2103 343 Size: 778 190 792 1569 Fragments arranged by size: 1569 792 778 190 Bsp1286I G_dGCh'C Cuts at: 343 954 1047 1121 1311 1373 2103 343 Size: 611 93 74 190 62 730 1569 Fragments arranged by size: 1569 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2549 2549 Size: 3329 BspHI T'CATG_A Cuts at: 2505 2505 Size: 3329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 379 593 895 1276 379 Size: 214 302 381 2432 Fragments arranged by size: 2432 381 302 214 BsrBI CCG'CTC Cuts at: 50 1621 1675 3159 3324 50 Size: 1571 54 1484 165 55 Fragments arranged by size: 1571 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1241 1241 Size: 3329 BsrFI r'CCGG_y Cuts at: 1327 1508 1717 1327 Size: 181 209 2939 Fragments arranged by size: 2939 209 181 BssSI C'ACGA_G Cuts at: 1600 1958 1600 Size: 358 2971 BstAPI GCAn_nnn'nTGC Cuts at: 706 706 Size: 3329 BstBI TT'CG_AA Cuts at: 205 1690 205 Size: 1485 1844 BstYI r'GATC_y Cuts at: 1 354 821 844 1179 1425 2437 2523 2535 Size: 353 467 23 335 246 1012 86 12 Cuts at: 2535 2881 1 Size: 346 449 Fragments arranged by size: 1012 467 449 353 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1279 1460 1623 1279 Size: 181 163 2985 Fragments arranged by size: 2985 181 163 BtsI GCAGTG_nn' Cuts at: 572 572 Size: 3329 DraI TTT'AAA Cuts at: 328 328 Size: 3329 DrdI GACnn_nn'nnGTC Cuts at: 1035 1893 1035 Size: 858 2471 EaeI y'GGCC_r Cuts at: 914 1088 1479 1506 2851 914 Size: 174 391 27 1345 1392 Fragments arranged by size: 1392 1345 391 174 27 EagI C'GGCC_G Cuts at: 914 914 Size: 3329 EarI CTCTTCn'nnn_ Cuts at: 1352 1562 1352 Size: 210 3119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1537 1847 1993 3139 1537 Size: 310 146 1146 1727 Fragments arranged by size: 1727 1146 310 146 EcoICRI GAG'CTC Cuts at: 341 341 Size: 3329 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 266 1154 1586 2333 2601 266 Size: 888 432 747 268 994 Fragments arranged by size: 994 888 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 468 468 Size: 3329 EcoRI G'AATT_C Cuts at: 333 333 Size: 3329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1348 1348 Size: 3329 FspI TGC'GCA Cuts at: 1110 1110 Size: 3329 HaeII r_GCGC'y Cuts at: 763 1011 2033 3012 763 Size: 248 1022 979 1080 Fragments arranged by size: 1080 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 211 243 1171 1201 1203 1233 211 Size: 32 928 30 2 30 2307 Fragments arranged by size: 2307 928 32 30 30 2 HincII GTy'rAC Cuts at: 368 368 Size: 3329 KasI G'GCGC_C Cuts at: 759 1007 759 Size: 248 3081 KpnI G_GTAC'C Cuts at: 349 349 Size: 3329 MluI A'CGCG_T Cuts at: 2762 2762 Size: 3329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2000 2184 2000 Size: 184 3145 MscI TGG'CCA Cuts at: 1090 1090 Size: 3329 MslI CAynn'nnrTG Cuts at: 1318 1445 1318 Size: 127 3202 MspA1I CmG'CkG Cuts at: 217 440 754 1114 2127 2372 217 Size: 223 314 360 1013 245 1174 Fragments arranged by size: 1174 1013 360 314 245 223 NaeI GCC'GGC Cuts at: 1510 1510 Size: 3329 NarI GG'CG_CC Cuts at: 760 1008 760 Size: 248 3081 NgoMIV G'CCGG_C Cuts at: 1508 1508 Size: 3329 NspI r_CATG'y Cuts at: 247 382 882 1789 247 Size: 135 500 907 1787 Fragments arranged by size: 1787 907 500 135 PciI A'CATG_T Cuts at: 1785 1785 Size: 3329 PmeI GTTT'AAAC Cuts at: 328 328 Size: 3329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2468 2500 2468 Size: 32 3297 PstI C_TGCA'G Cuts at: 376 1061 376 Size: 685 2644 PvuI CG_AT'CG Cuts at: 104 104 Size: 3329 PvuII CAG'CTG Cuts at: 754 1114 754 Size: 360 2969 RsrII CG'GwC_CG Cuts at: 1524 1524 Size: 3329 SacI G_AGCT'C Cuts at: 343 343 Size: 3329 SalI G'TCGA_C Cuts at: 366 366 Size: 3329 SapI GCTCTTCn'nnn_ Cuts at: 1352 1562 1352 Size: 210 3119 SbfI CC_TGCA'GG Cuts at: 376 376 Size: 3329 ScaI AGT'ACT Cuts at: 2846 2846 Size: 3329 SfcI C'TryA_G Cuts at: 33 372 1057 2050 2241 33 Size: 339 685 993 191 1121 Fragments arranged by size: 1121 993 685 339 191 SfoI GGC'GCC Cuts at: 761 1009 761 Size: 248 3081 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3329 SmaI CCC'GGG Cuts at: 351 351 Size: 3329 SmlI C'TyrA_G Cuts at: 484 1399 1891 2153 484 Size: 915 492 262 1660 Fragments arranged by size: 1660 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3329 SphI G_CATG'C Cuts at: 382 882 382 Size: 500 2829 StyI C'CwwG_G Cuts at: 463 817 463 Size: 354 2975 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 912 1045 1504 1713 912 Size: 133 459 209 2528 Fragments arranged by size: 2528 459 209 133 TatI w'GTAC_w Cuts at: 2844 2844 Size: 3329 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2846 2878 2846 Size: 32 3297 Tth111I GACn'n_nGTC Cuts at: 1126 1126 Size: 3329 XbaI T'CTAG_A Cuts at: 360 360 Size: 3329 XcmI CCAnnnn_n'nnnnTGG Cuts at: 824 824 Size: 3329 XmaI C'CCGG_G Cuts at: 349 349 Size: 3329 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI