Restriction map for pF1KB5643
 (Circular) (Six-base) MAPSORT of: pF1KB5643.seq  Check: 561  from: 1  to: 3329



pF1KB5643  3329 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AccI GT'mk_AC

Cuts at:    367     367 
   Size:       3329
 
Acc65I G'GTAC_C

Cuts at:    345     345 
   Size:       3329
 
AclI AA'CG_TT

Cuts at:   3169    3169 
   Size:       3329
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    266    1154    1586    2333    2601     266 
   Size:        888     432     747     268     994

  Fragments arranged by size: 

                 994     888     747     432     268
 
AflIII A'CryG_T

Cuts at:    681    1785    2762     681 
   Size:       1104     977    1248

  Fragments arranged by size: 

                1248    1104     977
 
AgeI A'CCGG_T

Cuts at:   1717    1717 
   Size:       3329
 
AhdI GACnn_n'nnGTC

Cuts at:    225     225 
   Size:       3329
 
AlwNI CAG_nnn'CTG

Cuts at:    634    2201     634 
   Size:       1567    1762
 
ApaLI G'TGCA_C

Cuts at:   2099    2099 
   Size:       3329
 
ApoI r'AATT_y

Cuts at:    333    2682    2938     333 
   Size:       2349     256     724

  Fragments arranged by size: 

                2349     724     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3329
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3329
 
AvaI C'yCGr_G

Cuts at:    349     349 
   Size:       3329
 
BamHI G'GATC_C

Cuts at:    354     354 
   Size:       3329
 
BanI G'GyrC_C

Cuts at:    345     759    1007    1042     345 
   Size:        414     248      35    2632

  Fragments arranged by size: 

                2632     414     248      35
 
BanII G_rGCy'C

Cuts at:    343    1373     343 
   Size:       1030    2299
 
BbeI G_GCGC'C

Cuts at:    763    1011     763 
   Size:        248    3081
 
BciVI GTATCCnnnnn_n'

Cuts at:   1227    1988    3329    1227 
   Size:        761    1341    1227

  Fragments arranged by size: 

                1341    1227     761
 
BclI T'GATC_A

Cuts at:    849     849 
   Size:       3329
 
BglII A'GATC_T

Cuts at:    844    2881     844 
   Size:       2037    1292
 
BlpI GC'TnA_GC

Cuts at:    441     441 
   Size:       3329
 
Bme1580I G_kGCm'C

Cuts at:    954    1047    2103     954 
   Size:         93    1056    2180

  Fragments arranged by size: 

                2180    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    541     622     957    3101     541 
   Size:         81     335    2144     769

  Fragments arranged by size: 

                2144     769     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1696    1696 
   Size:       3329
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    505    1384    1876    2174     505 
   Size:        879     492     298    1660

  Fragments arranged by size: 

                1660     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3033    3033 
   Size:       3329
 
BsaAI yAC'GTr

Cuts at:    682     682 
   Size:       3329
 
BsaBI GATnn'nnATC

Cuts at:      6     843    2880       6 
   Size:        837    2037     455

  Fragments arranged by size: 

                2037     837     455
 
BsaHI Gr'CG_yC

Cuts at:    760    1008    1710    3206     760 
   Size:        248     702    1496     883

  Fragments arranged by size: 

                1496     883     702     248
 
BsaWI w'CCGG_w

Cuts at:    518    1039    1717    1991    2138    2549     518 
   Size:        521     678     274     147     411    1298

  Fragments arranged by size: 

                1298     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    754    1512    2089    2585     754 
   Size:        758     577     496    1498

  Fragments arranged by size: 

                1498     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     181 
   Size:       3329
 
BsiEI CG_ry'CG

Cuts at:    104     871     917    2125     104 
   Size:        767      46    1208    1308

  Fragments arranged by size: 

                1308    1208     767      46
 
BsiHKAI G_wGCw'C

Cuts at:    343    1121    1311    2103     343 
   Size:        778     190     792    1569

  Fragments arranged by size: 

                1569     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    343     954    1047    1121    1311    1373    2103     343 
   Size:        611      93      74     190      62     730    1569

  Fragments arranged by size: 

                1569     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2549    2549 
   Size:       3329
 
BspHI T'CATG_A

Cuts at:   2505    2505 
   Size:       3329
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    379     593     895    1276     379 
   Size:        214     302     381    2432

  Fragments arranged by size: 

                2432     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1621    1675    3159    3324      50 
   Size:       1571      54    1484     165      55

  Fragments arranged by size: 

                1571    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1241    1241 
   Size:       3329
 
BsrFI r'CCGG_y

Cuts at:   1327    1508    1717    1327 
   Size:        181     209    2939

  Fragments arranged by size: 

                2939     209     181
 
BssSI C'ACGA_G

Cuts at:   1600    1958    1600 
   Size:        358    2971
 
BstAPI GCAn_nnn'nTGC

Cuts at:    706     706 
   Size:       3329
 
BstBI TT'CG_AA

Cuts at:    205    1690     205 
   Size:       1485    1844
 
BstYI r'GATC_y

Cuts at:      1     354     821     844    1179    1425    2437    2523    2535 
   Size:        353     467      23     335     246    1012      86      12

Cuts at:   2535    2881       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     353     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1279    1460    1623    1279 
   Size:        181     163    2985

  Fragments arranged by size: 

                2985     181     163
 
BtsI GCAGTG_nn'

Cuts at:    572     572 
   Size:       3329
 
DraI TTT'AAA

Cuts at:    328     328 
   Size:       3329
 
DrdI GACnn_nn'nnGTC

Cuts at:   1035    1893    1035 
   Size:        858    2471
 
EaeI y'GGCC_r

Cuts at:    914    1088    1479    1506    2851     914 
   Size:        174     391      27    1345    1392

  Fragments arranged by size: 

                1392    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    914     914 
   Size:       3329
 
EarI CTCTTCn'nnn_

Cuts at:   1352    1562    1352 
   Size:        210    3119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1537    1847    1993    3139    1537 
   Size:        310     146    1146    1727

  Fragments arranged by size: 

                1727    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    341     341 
   Size:       3329
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    266    1154    1586    2333    2601     266 
   Size:        888     432     747     268     994

  Fragments arranged by size: 

                 994     888     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    468     468 
   Size:       3329
 
EcoRI G'AATT_C

Cuts at:    333     333 
   Size:       3329
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1348    1348 
   Size:       3329
 
FspI TGC'GCA

Cuts at:   1110    1110 
   Size:       3329
 
HaeII r_GCGC'y

Cuts at:    763    1011    2033    3012     763 
   Size:        248    1022     979    1080

  Fragments arranged by size: 

                1080    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    211     243    1171    1201    1203    1233     211 
   Size:         32     928      30       2      30    2307

  Fragments arranged by size: 

                2307     928      32      30      30       2
 
HincII GTy'rAC

Cuts at:    368     368 
   Size:       3329
 
KasI G'GCGC_C

Cuts at:    759    1007     759 
   Size:        248    3081
 
KpnI G_GTAC'C

Cuts at:    349     349 
   Size:       3329
 
MluI A'CGCG_T

Cuts at:   2762    2762 
   Size:       3329
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2000    2184    2000 
   Size:        184    3145
 
MscI TGG'CCA

Cuts at:   1090    1090 
   Size:       3329
 
MslI CAynn'nnrTG

Cuts at:   1318    1445    1318 
   Size:        127    3202
 
MspA1I CmG'CkG

Cuts at:    217     440     754    1114    2127    2372     217 
   Size:        223     314     360    1013     245    1174

  Fragments arranged by size: 

                1174    1013     360     314     245     223
 
NaeI GCC'GGC

Cuts at:   1510    1510 
   Size:       3329
 
NarI GG'CG_CC

Cuts at:    760    1008     760 
   Size:        248    3081
 
NgoMIV G'CCGG_C

Cuts at:   1508    1508 
   Size:       3329
 
NspI r_CATG'y

Cuts at:    247     382     882    1789     247 
   Size:        135     500     907    1787

  Fragments arranged by size: 

                1787     907     500     135
 
PciI A'CATG_T

Cuts at:   1785    1785 
   Size:       3329
 
PmeI GTTT'AAAC

Cuts at:    328     328 
   Size:       3329
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2468    2500    2468 
   Size:         32    3297
 
PstI C_TGCA'G

Cuts at:    376    1061     376 
   Size:        685    2644
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3329
 
PvuII CAG'CTG

Cuts at:    754    1114     754 
   Size:        360    2969
 
RsrII CG'GwC_CG

Cuts at:   1524    1524 
   Size:       3329
 
SacI G_AGCT'C

Cuts at:    343     343 
   Size:       3329
 
SalI G'TCGA_C

Cuts at:    366     366 
   Size:       3329
 
SapI GCTCTTCn'nnn_

Cuts at:   1352    1562    1352 
   Size:        210    3119
 
SbfI CC_TGCA'GG

Cuts at:    376     376 
   Size:       3329
 
ScaI AGT'ACT

Cuts at:   2846    2846 
   Size:       3329
 
SfcI C'TryA_G

Cuts at:     33     372    1057    2050    2241      33 
   Size:        339     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     339     191
 
SfoI GGC'GCC

Cuts at:    761    1009     761 
   Size:        248    3081
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3329
 
SmaI CCC'GGG

Cuts at:    351     351 
   Size:       3329
 
SmlI C'TyrA_G

Cuts at:    484    1399    1891    2153     484 
   Size:        915     492     262    1660

  Fragments arranged by size: 

                1660     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3329
 
SphI G_CATG'C

Cuts at:    382     882     382 
   Size:        500    2829
 
StyI C'CwwG_G

Cuts at:    463     817     463 
   Size:        354    2975
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    912    1045    1504    1713     912 
   Size:        133     459     209    2528

  Fragments arranged by size: 

                2528     459     209     133
 
TatI w'GTAC_w

Cuts at:   2844    2844 
   Size:       3329
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2846    2878    2846 
   Size:         32    3297
 
Tth111I GACn'n_nGTC

Cuts at:   1126    1126 
   Size:       3329
 
XbaI T'CTAG_A

Cuts at:    360     360 
   Size:       3329
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    824     824 
   Size:       3329
 
XmaI C'CCGG_G

Cuts at:    349     349 
   Size:       3329
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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