(Circular) (Six-base) MAPSORT of: pF1KB5566.seq Check: 5673 from: 1 to: 3905 pF1KB5566 3905 bp With 182 enzymes: SgfI * June 20, 2008 10:25 .. AccI GT'mk_AC Cuts at: 853 943 853 Size: 90 3815 Acc65I G'GTAC_C Cuts at: 921 921 Size: 3905 AclI AA'CG_TT Cuts at: 3745 3745 Size: 3905 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1730 2162 2909 3177 1730 Size: 432 747 268 2458 Fragments arranged by size: 2458 747 432 268 AflIII A'CryG_T Cuts at: 1257 2361 3338 1257 Size: 1104 977 1824 Fragments arranged by size: 1824 1104 977 AgeI A'CCGG_T Cuts at: 797 2293 797 Size: 1496 2409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 781 781 Size: 3905 AlwNI CAG_nnn'CTG Cuts at: 687 1210 2777 687 Size: 523 1567 1815 Fragments arranged by size: 1815 1567 523 ApaLI G'TGCA_C Cuts at: 2675 2675 Size: 3905 ApoI r'AATT_y Cuts at: 909 3258 3514 909 Size: 2349 256 1300 Fragments arranged by size: 2349 1300 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3905 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3905 AvaI C'yCGr_G Cuts at: 925 925 Size: 3905 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 369 402 369 Size: 33 3872 BamHI G'GATC_C Cuts at: 204 325 930 204 Size: 121 605 3179 Fragments arranged by size: 3179 605 121 BanI G'GyrC_C Cuts at: 397 921 1335 1583 1618 397 Size: 524 414 248 35 2684 Fragments arranged by size: 2684 524 414 248 35 BanII G_rGCy'C Cuts at: 919 1949 919 Size: 1030 2875 BbeI G_GCGC'C Cuts at: 1339 1587 1339 Size: 248 3657 BciVI GTATCCnnnnn_n' Cuts at: 1803 2564 3905 1803 Size: 761 1341 1803 Fragments arranged by size: 1803 1341 761 BclI T'GATC_A Cuts at: 884 1425 884 Size: 541 3364 BglII A'GATC_T Cuts at: 1420 3457 1420 Size: 2037 1868 BlpI GC'TnA_GC Cuts at: 680 721 1017 680 Size: 41 296 3568 Fragments arranged by size: 3568 296 41 Bme1580I G_kGCm'C Cuts at: 1530 1623 2679 1530 Size: 93 1056 2756 Fragments arranged by size: 2756 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 876 1117 1198 1533 3677 876 Size: 241 81 335 2144 1104 Fragments arranged by size: 2144 1104 335 241 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 3905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 724 724 Size: 3905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1081 1960 2452 2750 1081 Size: 879 492 298 2236 Fragments arranged by size: 2236 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 853 3609 853 Size: 2756 1149 BsaAI yAC'GTr Cuts at: 1258 1258 Size: 3905 BsaBI GATnn'nnATC Cuts at: 6 1419 3456 6 Size: 1413 2037 455 Fragments arranged by size: 2037 1413 455 BsaHI Gr'CG_yC Cuts at: 211 1336 1584 2286 3782 211 Size: 1125 248 702 1496 334 Fragments arranged by size: 1496 1125 702 334 248 BsaWI w'CCGG_w Cuts at: 133 797 1094 1615 2293 2567 2714 3125 133 Size: 664 297 521 678 274 147 411 913 Fragments arranged by size: 913 678 664 521 411 297 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 478 478 Size: 3905 BseYI C'CCAG_C Cuts at: 321 393 1330 2088 2665 3161 321 Size: 72 937 758 577 496 1065 Fragments arranged by size: 1065 937 758 577 496 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 200 200 Size: 3905 BsiEI CG_ry'CG Cuts at: 104 1447 1493 2701 104 Size: 1343 46 1208 1308 Fragments arranged by size: 1343 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 919 1697 1887 2679 919 Size: 778 190 792 2145 Fragments arranged by size: 2145 792 778 190 BsmI GAATG_Cn' Cuts at: 176 176 Size: 3905 Bsp1286I G_dGCh'C Cuts at: 919 1530 1623 1697 1887 1949 2679 919 Size: 611 93 74 190 62 730 2145 Fragments arranged by size: 2145 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 133 3125 133 Size: 2992 913 BspHI T'CATG_A Cuts at: 3081 3081 Size: 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 955 1169 1471 1852 955 Size: 214 302 381 3008 Fragments arranged by size: 3008 381 302 214 BsrBI CCG'CTC Cuts at: 50 2197 2251 3735 3900 50 Size: 2147 54 1484 165 55 Fragments arranged by size: 2147 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1817 1817 Size: 3905 BsrFI r'CCGG_y Cuts at: 536 797 1903 2084 2293 536 Size: 261 1106 181 209 2148 Fragments arranged by size: 2148 1106 261 209 181 BsrGI T'GTAC_A Cuts at: 530 530 Size: 3905 BssSI C'ACGA_G Cuts at: 2176 2534 2176 Size: 358 3547 BstAPI GCAn_nnn'nTGC Cuts at: 717 1282 717 Size: 565 3340 BstBI TT'CG_AA Cuts at: 2266 2266 Size: 3905 BstEII G'GTnAC_C Cuts at: 258 258 Size: 3905 BstYI r'GATC_y Cuts at: 1 204 325 930 1397 1420 1755 2001 3013 Size: 203 121 605 467 23 335 246 1012 Cuts at: 3013 3099 3111 3457 1 Size: 86 12 346 449 Fragments arranged by size: 1012 605 467 449 346 335 246 203 121 86 23 12 BtgI C'CryG_G Cuts at: 107 254 569 107 Size: 147 315 3443 Fragments arranged by size: 3443 315 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1855 2036 2199 1855 Size: 181 163 3561 Fragments arranged by size: 3561 181 163 BtsI GCAGTG_nn' Cuts at: 1148 1148 Size: 3905 DraI TTT'AAA Cuts at: 904 904 Size: 3905 DrdI GACnn_nn'nnGTC Cuts at: 1611 2469 1611 Size: 858 3047 EaeI y'GGCC_r Cuts at: 444 653 1490 1664 2055 2082 3427 444 Size: 209 837 174 391 27 1345 922 Fragments arranged by size: 1345 922 837 391 209 174 27 EagI C'GGCC_G Cuts at: 1490 1490 Size: 3905 EarI CTCTTCn'nnn_ Cuts at: 1928 2138 1928 Size: 210 3695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 311 2113 2423 2569 3715 311 Size: 1802 310 146 1146 501 Fragments arranged by size: 1802 1146 501 310 146 EcoICRI GAG'CTC Cuts at: 917 917 Size: 3905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 724 1730 2162 2909 3177 724 Size: 1006 432 747 268 1452 Fragments arranged by size: 1452 1006 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 266 1044 266 Size: 778 3127 EcoRI G'AATT_C Cuts at: 909 909 Size: 3905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1924 1924 Size: 3905 FspI TGC'GCA Cuts at: 1686 1686 Size: 3905 HaeII r_GCGC'y Cuts at: 543 1339 1587 2609 3588 543 Size: 796 248 1022 979 860 Fragments arranged by size: 1022 979 860 796 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 196 218 228 250 1747 1777 1779 1809 196 Size: 22 10 22 1497 30 2 30 2292 Fragments arranged by size: 2292 1497 30 30 22 22 10 2 HincII GTy'rAC Cuts at: 378 944 378 Size: 566 3339 KasI G'GCGC_C Cuts at: 1335 1583 1335 Size: 248 3657 KpnI G_GTAC'C Cuts at: 925 925 Size: 3905 MluI A'CGCG_T Cuts at: 3338 3338 Size: 3905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2576 2760 2576 Size: 184 3721 MscI TGG'CCA Cuts at: 655 1666 655 Size: 1011 2894 MslI CAynn'nnrTG Cuts at: 763 1894 2021 763 Size: 1131 127 2647 Fragments arranged by size: 2647 1131 127 MspA1I CmG'CkG Cuts at: 1016 1330 1690 2703 2948 1016 Size: 314 360 1013 245 1973 Fragments arranged by size: 1973 1013 360 314 245 NaeI GCC'GGC Cuts at: 538 2086 538 Size: 1548 2357 NarI GG'CG_CC Cuts at: 1336 1584 1336 Size: 248 3657 NcoI C'CATG_G Cuts at: 107 254 569 107 Size: 147 315 3443 Fragments arranged by size: 3443 315 147 NgoMIV G'CCGG_C Cuts at: 536 2084 536 Size: 1548 2357 NspI r_CATG'y Cuts at: 321 624 762 958 1458 2365 321 Size: 303 138 196 500 907 1861 Fragments arranged by size: 1861 907 500 303 196 138 PciI A'CATG_T Cuts at: 2361 2361 Size: 3905 PmeI GTTT'AAAC Cuts at: 904 904 Size: 3905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3044 3076 3044 Size: 32 3873 PpuMI rG'GwC_Cy Cuts at: 266 266 Size: 3905 PshAI GACnn'nnGTC Cuts at: 271 849 271 Size: 578 3327 PstI C_TGCA'G Cuts at: 952 1637 952 Size: 685 3220 PvuI CG_AT'CG Cuts at: 104 104 Size: 3905 PvuII CAG'CTG Cuts at: 1330 1690 1330 Size: 360 3545 RsrII CG'GwC_CG Cuts at: 131 2100 131 Size: 1969 1936 SacI G_AGCT'C Cuts at: 919 919 Size: 3905 SalI G'TCGA_C Cuts at: 942 942 Size: 3905 SapI GCTCTTCn'nnn_ Cuts at: 1928 2138 1928 Size: 210 3695 SbfI CC_TGCA'GG Cuts at: 952 952 Size: 3905 ScaI AGT'ACT Cuts at: 3422 3422 Size: 3905 SfcI C'TryA_G Cuts at: 33 489 854 948 1633 2626 2817 33 Size: 456 365 94 685 993 191 1121 Fragments arranged by size: 1121 993 685 456 365 191 94 SfoI GGC'GCC Cuts at: 1337 1585 1337 Size: 248 3657 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3905 SmaI CCC'GGG Cuts at: 927 927 Size: 3905 SmlI C'TyrA_G Cuts at: 1060 1975 2467 2729 1060 Size: 915 492 262 2236 Fragments arranged by size: 2236 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3905 SphI G_CATG'C Cuts at: 762 958 1458 762 Size: 196 500 3209 Fragments arranged by size: 3209 500 196 StyI C'CwwG_G Cuts at: 107 254 338 569 1039 1393 107 Size: 147 84 231 470 354 2619 Fragments arranged by size: 2619 470 354 231 147 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 385 745 1488 1621 2080 2289 385 Size: 360 743 133 459 209 2001 Fragments arranged by size: 2001 743 459 360 209 133 TatI w'GTAC_w Cuts at: 530 770 3420 530 Size: 240 2650 1015 Fragments arranged by size: 2650 1015 240 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3422 3454 3422 Size: 32 3873 Tth111I GACn'n_nGTC Cuts at: 1702 1702 Size: 3905 XbaI T'CTAG_A Cuts at: 936 936 Size: 3905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 886 1400 886 Size: 514 3391 XmaI C'CCGG_G Cuts at: 925 925 Size: 3905 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI