Restriction map for pF1KB5523
 (Linear) (Six-base) MAPSORT of: KIBB5523.seq  Check: 9923  from: 1  to: 7416



KIBB5523.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1422    3192    7416 
   Size:       1422    1770    4224

  Fragments arranged by size: 

                4224    1770    1422
 
AatII G_ACGT'C

Cuts at:      0    4605    7416 
   Size:       4605    2811
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     179     229     290     542     739    1208    2806 
   Size:        175       4      50      61     252     197     469    1598

Cuts at:   2806    3439    3482    3497    5161    5584    5746    5920    7416 
   Size:        633      43      15    1664     423     162     174    1496

  Fragments arranged by size: 

                1664    1598    1496     633     469     423     252     197
                 175     174     162      61      50      43      15       4
            
 
AfeI AGC'GCT

Cuts at:      0     775    1511    3640    4027    7416 
   Size:        775     736    2129     387    3389

  Fragments arranged by size: 

                3389    2129     775     736     387
 
AflII C'TTAA_G

Cuts at:      0    1516    7416 
   Size:       1516    5900
 
AflIII A'CryG_T

Cuts at:      0    6141    7416 
   Size:       6141    1275
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3471    4053    5328    7416 
   Size:       3471     582    1275    2088

  Fragments arranged by size: 

                3471    2088    1275     582
 
AleI CACnn'nnGTG

Cuts at:      0    1220    7416 
   Size:       1220    6196
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1420    2702    2880    3145    4333    4535    6925    7416 
   Size:       1420    1282     178     265    1188     202    2390     491

  Fragments arranged by size: 

                2390    1420    1282    1188     491     265     202     178
            
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    7195    7227    7416 
   Size:       7195      32     189

  Fragments arranged by size: 

                7195     189      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     540     717     876    1278    2337    2463    2655 
   Size:        117     423     177     159     402    1059     126     192

Cuts at:   2655    3660    3810    4212    4959    5403    5532    5595    6204 
   Size:       1005     150     402     747     444     129      63     609

Cuts at:   6204    7296    7416 
   Size:       1092     120

  Fragments arranged by size: 

                1092    1059    1005     747     609     444     423     402
                 402     192     177     159     150     129     126     120
                 117      63
 
ApaI G_GGCC'C

Cuts at:      0    1701    7245    7416 
   Size:       1701    5544     171

  Fragments arranged by size: 

                5544    1701     171
 
ApaLI G'TGCA_C

Cuts at:      0    3939    7416 
   Size:       3939    3477
 
ApoI r'AATT_y

Cuts at:      0     568    1582    6979    7416 
   Size:        568    1014    5397     437

  Fragments arranged by size: 

                5397    1014     568     437
 
AvaI C'yCGr_G

Cuts at:      0    2948    3092    3910    4388    5582    7301    7416 
   Size:       2948     144     818     478    1194    1719     115

  Fragments arranged by size: 

                2948    1719    1194     818     478     144     115
 
BamHI G'GATC_C

Cuts at:      0    4740    4814    6659    7134    7416 
   Size:       4740      74    1845     475     282

  Fragments arranged by size: 

                4740    1845     475     282      74
 
BanII G_rGCy'C

Cuts at:      0    1701    1754    1862    2315    2414    5365    7245    7286 
   Size:       1701      53     108     453      99    2951    1880      41

Cuts at:   7286    7367    7416 
   Size:         81      49

  Fragments arranged by size: 

                2951    1880    1701     453     108      99      81      53
                  49      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     929    1247    1484    1535    2771    3470    3929    4418 
   Size:        929     318     237      51    1236     699     459     489

Cuts at:   4418    4469    6004    6017    7416 
   Size:         51    1535      13    1399

  Fragments arranged by size: 

                1535    1399    1236     929     699     489     459     318
                 237      51      51      13
 
BbvCI CC'TCA_GC

Cuts at:      0     947    2774    4121    6418    7246    7416 
   Size:        947    1827    1347    2297     828     170

  Fragments arranged by size: 

                2297    1827    1347     947     828     170
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2032    2066    2752    2786    5169    5203    5686    5720 
   Size:       2032      34     686      34    2383      34     483      34

Cuts at:   5720    7416 
   Size:       1696

  Fragments arranged by size: 

                2383    2032    1696     686     483      34      34      34
                  34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     170    7416 
   Size:        170    7246
 
BclI T'GATC_A

Cuts at:      0     674    1967    4448    7184    7416 
   Size:        674    1293    2481    2736     232

  Fragments arranged by size: 

                2736    2481    1293     674     232
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2287    4393    4897    5041    6163    6295    7240    7416 
   Size:       2287    2106     504     144    1122     132     945     176

  Fragments arranged by size: 

                2287    2106    1122     945     504     176     144     132
            
 
BglII A'GATC_T

Cuts at:      0     906    2124    2811    7416 
   Size:        906    1218     687    4605

  Fragments arranged by size: 

                4605    1218     906     687
 
BlpI GC'TnA_GC

Cuts at:      0    1856    7416 
   Size:       1856    5560
 
Bme1580I G_kGCm'C

Cuts at:      0    1554    1701    3943    7245    7416 
   Size:       1554     147    2242    3302     171

  Fragments arranged by size: 

                3302    2242    1554     171     147
 
BmgBI CAC'GTC

Cuts at:      0     480    2393    5958    7416 
   Size:        480    1913    3565    1458

  Fragments arranged by size: 

                3565    1913    1458     480
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1035    2909    3075    3244    7416 
   Size:       1035    1874     166     169    4172

  Fragments arranged by size: 

                4172    1874    1035     169     166
 
BmtI G_CTAG'C

Cuts at:      0    1708    4102    7416 
   Size:       1708    2394    3314

  Fragments arranged by size: 

                3314    2394    1708
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5856    7416 
   Size:       5856    1560
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     562     919    1378    2131    2677    3127    3370 
   Size:        187     375     357     459     753     546     450     243

Cuts at:   3370    3490    3981    4153    4309    5902    6259    6544    6555 
   Size:        120     491     172     156    1593     357     285      11

Cuts at:   6555    6592    6943    7416 
   Size:         37     351     473

  Fragments arranged by size: 

                1593     753     546     491     473     459     450     375
                 357     357     351     285     243     187     172     156
                 120      37      11
 
Bpu10I CC'TnA_GC

Cuts at:      0     947    2774    4033    4121    4905    6418    7246    7416 
   Size:        947    1827    1259      88     784    1513     828     170

  Fragments arranged by size: 

                1827    1513    1259     947     828     784     170      88
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1738    2290    7416 
   Size:       1738     552    5126

  Fragments arranged by size: 

                5126    1738     552
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     848    6011    6523    6661    7416 
   Size:        848    5163     512     138     755

  Fragments arranged by size: 

                5163     848     755     512     138
 
BsaAI yAC'GTr

Cuts at:      0    3060    7269    7344    7386    7416 
   Size:       3060    4209      75      42      30

  Fragments arranged by size: 

                4209    3060      75      42      30
 
BsaBI GATnn'nnATC

Cuts at:      0     128    7416 
   Size:        128    7288
 
BsaHI Gr'CG_yC

Cuts at:      0     108    4602    6401    7416 
   Size:        108    4494    1799    1015

  Fragments arranged by size: 

                4494    1799    1015     108
 
BsaWI w'CCGG_w

Cuts at:      0    4114    4151    4811    7416 
   Size:       4114      37     660    2605

  Fragments arranged by size: 

                4114    2605     660      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     281     311    7309    7339    7416 
   Size:        281      30    6998      30      77

  Fragments arranged by size: 

                6998     281      77      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     679    1354    2401    3124    3364    3508    5341    5530 
   Size:        679     675    1047     723     240     144    1833     189

Cuts at:   5530    5845    6031    6346    6402    6541    6616    6673    6832 
   Size:        315     186     315      56     139      75      57     159

Cuts at:   6832    6979    7234    7294    7416 
   Size:        147     255      60     122

  Fragments arranged by size: 

                1833    1047     723     679     675     315     315     255
                 240     189     186     159     147     144     139     122
                  75      60      57      56
 
BseYI C'CCAG_C

Cuts at:      0     528    1124    1180    1700    2639    2675    6067    6632 
   Size:        528     596      56     520     939      36    3392     565

Cuts at:   6632    6728    6872    6897    7416 
   Size:         96     144      25     519

  Fragments arranged by size: 

                3392     939     596     565     528     520     519     144
                  96      56      36      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     364     475    2158    3520    4001    4301    5281 
   Size:        295      69     111    1683    1362     481     300     980

Cuts at:   5281    5416    5772    6052    7416 
   Size:        135     356     280    1364

  Fragments arranged by size: 

                1683    1364    1362     980     481     356     300     295
                 280     135     111      69
 
BsiEI CG_ry'CG

Cuts at:      0    3033    5379    7416 
   Size:       3033    2346    2037

  Fragments arranged by size: 

                3033    2346    2037
 
BsiHKAI G_wGCw'C

Cuts at:      0    1217    1754    1862    3386    3943    5552    6854    7286 
   Size:       1217     537     108    1524     557    1609    1302     432

Cuts at:   7286    7367    7416 
   Size:         81      49

  Fragments arranged by size: 

                1609    1524    1302    1217     557     537     432     108
                  81      49
 
BsiWI C'GTAC_G

Cuts at:      0    3056    7416 
   Size:       3056    4360
 
BsmI GAATG_Cn'

Cuts at:      0    2547    7416 
   Size:       2547    4869
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2383    7329    7416 
   Size:       2383    4946      87

  Fragments arranged by size: 

                4946    2383      87
 
Bsp1286I G_dGCh'C

Cuts at:      0    1217    1554    1701    1754    1862    2315    2414    3386 
   Size:       1217     337     147      53     108     453      99     972

Cuts at:   3386    3943    5365    5552    6854    7245    7286    7367    7416 
   Size:        557    1422     187    1302     391      41      81      49

  Fragments arranged by size: 

                1422    1302    1217     972     557     453     391     337
                 187     147     108      99      81      53      49      41
            
 
BspHI T'CATG_A

Cuts at:      0     600    6182    7181    7416 
   Size:        600    5582     999     235

  Fragments arranged by size: 

                5582     999     600     235
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      51    1412    1422    3192    3363    3935    4028    5093 
   Size:         51    1361      10    1770     171     572      93    1065

Cuts at:   5093    7082    7416 
   Size:       1989     334

  Fragments arranged by size: 

                1989    1770    1361    1065     572     334     171      93
                  51      10
 
BsrBI CCG'CTC

Cuts at:      0    1348    3337    6410    7400    7416 
   Size:       1348    1989    3073     990      16

  Fragments arranged by size: 

                3073    1989    1348     990      16
 
BsrDI GCAATG_nn'

Cuts at:      0    1625    1671    2061    2414    3319    4126    4584    6095 
   Size:       1625      46     390     353     905     807     458    1511

Cuts at:   6095    7416 
   Size:       1321

  Fragments arranged by size: 

                1625    1511    1321     905     807     458     390     353
                  46
 
BsrFI r'CCGG_y

Cuts at:      0    2867    3048    3175    4540    4571    4867    5451    7416 
   Size:       2867     181     127    1365      31     296     584    1965

  Fragments arranged by size: 

                2867    1965    1365     584     296     181     127      31
            
 
BssSI C'ACGA_G

Cuts at:      0     889    1675    2284    2596    7416 
   Size:        889     786     609     312    4820

  Fragments arranged by size: 

                4820     889     786     609     312
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1282    2497    3507    5103    7416 
   Size:       1282    1215    1010    1596    2313

  Fragments arranged by size: 

                2313    1596    1282    1215    1010
 
BstEII G'GTnAC_C

Cuts at:      0    2955    7416 
   Size:       2955    4461
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1176    2239    7416 
   Size:       1176    1063    5177

  Fragments arranged by size: 

                5177    1176    1063
 
BstYI r'GATC_y

Cuts at:      0     537     906    1034    1806    2124    2811    4740    4814 
   Size:        537     369     128     772     318     687    1929      74

Cuts at:   4814    5519    6659    6932    7134    7416 
   Size:        705    1140     273     202     282

  Fragments arranged by size: 

                1929    1140     772     705     687     537     369     318
                 282     273     202     128      74
 
Bsu36I CC'TnA_GG

Cuts at:      0     103     662    5404    7416 
   Size:        103     559    4742    2012

  Fragments arranged by size: 

                4742    2012     559     103
 
BtgI C'CryG_G

Cuts at:      0    2315    2546    2885    3571    3602    4818    6512    6812 
   Size:       2315     231     339     686      31    1216    1694     300

Cuts at:   6812    6860    7079    7416 
   Size:         48     219     337

  Fragments arranged by size: 

                2315    1694    1216     686     339     337     300     231
                 219      48      31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4518    5275    7416 
   Size:       4518     757    2141

  Fragments arranged by size: 

                4518    2141     757
 
BtsI GCAGTG_nn'

Cuts at:      0    1276    1399    3127    3186    4878    5187    6107    6883 
   Size:       1276     123    1728      59    1692     309     920     776

Cuts at:   6883    7416 
   Size:        533

  Fragments arranged by size: 

                1728    1692    1276     920     776     533     309     123
                  59
 
ClaI AT'CG_AT

Cuts at:      0     132    4163    7416 
   Size:        132    4031    3253

  Fragments arranged by size: 

                4031    3253     132
 
DraIII CAC_nnn'GTG

Cuts at:      0    3603    7416 
   Size:       3603    3813
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    5758    7321    7416 
   Size:       5758    1563      95

  Fragments arranged by size: 

                5758    1563      95
 
EaeI y'GGCC_r

Cuts at:      0     849    1631    1902    2241    2339    3605    3662    4542 
   Size:        849     782     271     339      98    1266      57     880

Cuts at:   4542    5376    5678    6012    6206    6449    7149    7416 
   Size:        834     302     334     194     243     700     267

  Fragments arranged by size: 

                1266     880     849     834     782     700     339     334
                 302     271     267     243     194      98      57
 
EagI C'GGCC_G

Cuts at:      0    5376    7416 
   Size:       5376    2040
 
EarI CTCTTCn'nnn_

Cuts at:      0     155     518     569    1417    2371    2935    3259    3743 
   Size:        155     363      51     848     954     564     324     484

Cuts at:   3743    4487    5594    5633    5847    6181    6280    6701    7416 
   Size:        744    1107      39     214     334      99     421     715

  Fragments arranged by size: 

                1107     954     848     744     715     564     484     421
                 363     334     324     214     155      99      51      39
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    5968    7416 
   Size:       5968    1448
 
EcoICRI GAG'CTC

Cuts at:      0    1752    1860    7284    7365    7416 
   Size:       1752     108    5424      81      51

  Fragments arranged by size: 

                5424    1752     108      81      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     179     187     229     290     542     562     739 
   Size:        175       4       8      42      61     252      20     177

Cuts at:    739     919    1208    1378    2131    2677    2806    3127    3370 
   Size:        180     289     170     753     546     129     321     243

Cuts at:   3370    3439    3482    3490    3497    3981    4153    4309    5161 
   Size:         69      43       8       7     484     172     156     852

Cuts at:   5161    5584    5746    5902    5920    6259    6544    6555    6592 
   Size:        423     162     156      18     339     285      11      37

Cuts at:   6592    6943    7416 
   Size:        351     473

  Fragments arranged by size: 

                 852     753     546     484     473     423     351     339
                 321     289     285     252     243     180     177     175
                 172     170     162     156     156     129      69      61
                  43      42      37      20      18      11       8       8
                   7       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1336    2167    4291    7416 
   Size:       1336     831    2124    3125

  Fragments arranged by size: 

                3125    2124    1336     831
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     723    2652    3414    3807    3840    4047    5034 
   Size:         63     660    1929     762     393      33     207     987

Cuts at:   5034    5145    5382    5856    6270    6519    6692    6716    6798 
   Size:        111     237     474     414     249     173      24      82

Cuts at:   6798    6822    6969    7242    7416 
   Size:         24     147     273     174

  Fragments arranged by size: 

                1929     987     762     660     474     414     393     273
                 249     237     207     174     173     147     111      82
                  63      33      24      24
 
EcoRI G'AATT_C

Cuts at:      0    6979    7416 
   Size:       6979     437
 
EcoRV GAT'ATC

Cuts at:      0    2976    7416 
   Size:       2976    4440
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1531    6142    7416 
   Size:       1531    4611    1274

  Fragments arranged by size: 

                4611    1531    1274
 
FspI TGC'GCA

Cuts at:      0    1375    5444    6907    7416 
   Size:       1375    4069    1463     509

  Fragments arranged by size: 

                4069    1463    1375     509
 
FspAI rTGC'GCAy

Cuts at:      0    6907    7416 
   Size:       6907     509
 
HaeII r_GCGC'y

Cuts at:      0     777    1354    1513    3642    3695    3894    4029    5235 
   Size:        777     577     159    2129      53     199     135    1206

Cuts at:   5235    7235    7416 
   Size:       2000     181

  Fragments arranged by size: 

                2129    2000    1206     777     577     199     181     159
                 135      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2692    2724    2752    2784    2796    2828    3135    3167 
   Size:       2692      32      28      32      12      32     307      32

Cuts at:   3167    3457    3489    3776    3808    4039    4071    4308    4340 
   Size:        290      32     287      32     231      32     237      32

Cuts at:   4340    4491    4523    4615    4647    5314    5346    5789    5821 
   Size:        151      32      92      32     667      32     443      32

Cuts at:   5821    5908    5940    7030    7062    7416 
   Size:         87      32    1090      32     354

  Fragments arranged by size: 

                2692    1090     667     443     354     307     290     287
                 237     231     151      92      87      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      28      12
 
HincII GTy'rAC

Cuts at:      0    1923    7416 
   Size:       1923    5493
 
HindIII A'AGCT_T

Cuts at:      0     241    1796    2017    2590    2633    2798    5738    6232 
   Size:        241    1555     221     573      43     165    2940     494

Cuts at:   6232    6380    7416 
   Size:        148    1036

  Fragments arranged by size: 

                2940    1555    1036     573     494     241     221     165
                 148      43
 
MfeI C'AATT_G

Cuts at:      0     509    7416 
   Size:        509    6907
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     787    1838    3239    3336    4683    6515    7416 
   Size:        787    1051    1401      97    1347    1832     901

  Fragments arranged by size: 

                1832    1401    1347    1051     901     787      97
 
MscI TGG'CCA

Cuts at:      0    1633    1904    2243    3607    5680    6208    7151    7416 
   Size:       1633     271     339    1364    2073     528     943     265

  Fragments arranged by size: 

                2073    1633    1364     943     528     339     271     265
            
 
MslI CAynn'nnrTG

Cuts at:      0    1220    2249    3719    6907    7416 
   Size:       1220    1029    1470    3188     509

  Fragments arranged by size: 

                3188    1470    1220    1029     509
 
MspA1I CmG'CkG

Cuts at:      0     714    1124    3660    4212    4344    4942    5529    5571 
   Size:        714     410    2536     552     132     598     587      42

Cuts at:   5571    5578    5592    6314    6447    6591    6888    6897    7416 
   Size:          7      14     722     133     144     297       9     519

  Fragments arranged by size: 

                2536     722     714     598     587     552     519     410
                 297     144     133     132      42      14       9       7
            
 
NaeI GCC'GGC

Cuts at:      0    2869    3050    3177    4542    4573    4869    5453    7416 
   Size:       2869     181     127    1365      31     296     584    1963

  Fragments arranged by size: 

                2869    1963    1365     584     296     181     127      31
            
 
NcoI C'CATG_G

Cuts at:      0    2315    2546    6512    6812    7079    7416 
   Size:       2315     231    3966     300     267     337

  Fragments arranged by size: 

                3966    2315     337     300     267     231
 
NgoMIV G'CCGG_C

Cuts at:      0    2867    3048    3175    4540    4571    4867    5451    7416 
   Size:       2867     181     127    1365      31     296     584    1965

  Fragments arranged by size: 

                2867    1965    1365     584     296     181     127      31
            
 
NheI G'CTAG_C

Cuts at:      0    1704    4098    7416 
   Size:       1704    2394    3318

  Fragments arranged by size: 

                3318    2394    1704
 
NspI r_CATG'y

Cuts at:      0     550    1063    2161    3258    4801    6906    6912    7416 
   Size:        550     513    1098    1097    1543    2105       6     504

  Fragments arranged by size: 

                2105    1543    1098    1097     550     513     504       6
            
 
PasI CC'CwG_GG

Cuts at:      0    3539    7062    7416 
   Size:       3539    3523     354

  Fragments arranged by size: 

                3539    3523     354
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     450     540    3684    3740    6204    7416 
   Size:        450      90    3144      56    2464    1212

  Fragments arranged by size: 

                3144    2464    1212     450      90      56
 
PfoI T'CCnGG_A

Cuts at:      0      44    2369    2710    3104    4043    4487    5309    5332 
   Size:         44    2325     341     394     939     444     822      23

Cuts at:   5332    5468    5968    6830    7121    7416 
   Size:        136     500     862     291     295

  Fragments arranged by size: 

                2325     939     862     822     500     444     394     341
                 295     291     136      44      23
 
PpuMI rG'GwC_Cy

Cuts at:      0      63    2652    3414    3840    4047    5145    5382    6270 
   Size:         63    2589     762     426     207    1098     237     888

Cuts at:   6270    6692    6716    6798    7416 
   Size:        422      24      82     618

  Fragments arranged by size: 

                2589    1098     888     762     618     426     422     237
                 207      82      63      24
 
PsiI TTA'TAA

Cuts at:      0     215    1802    7416 
   Size:        215    1587    5614

  Fragments arranged by size: 

                5614    1587     215
 
PspOMI G'GGCC_C

Cuts at:      0    1697    7241    7416 
   Size:       1697    5544     175

  Fragments arranged by size: 

                5544    1697     175
 
PstI C_TGCA'G

Cuts at:      0     440     737    1260    1409    1727    2574    2672    3932 
   Size:        440     297     523     149     318     847      98    1260

Cuts at:   3932    4025    4343    4514    5895    6077    6581    7416 
   Size:         93     318     171    1381     182     504     835

  Fragments arranged by size: 

                1381    1260     847     835     523     504     440     318
                 318     297     182     171     149      98      93
 
PvuI CG_AT'CG

Cuts at:      0    3033    7416 
   Size:       3033    4383
 
PvuII CAG'CTG

Cuts at:      0    1124    3660    4212    4344    5571    5578    6314    6447 
   Size:       1124    2536     552     132    1227       7     736     133

Cuts at:   6447    6888    6897    7416 
   Size:        441       9     519

  Fragments arranged by size: 

                2536    1227    1124     736     552     519     441     133
                 132       9       7
 
SacI G_AGCT'C

Cuts at:      0    1754    1862    7286    7367    7416 
   Size:       1754     108    5424      81      49

  Fragments arranged by size: 

                5424    1754     108      81      49
 
SanDI GG'GwC_CC

Cuts at:      0    6716    7416 
   Size:       6716     700
 
SapI GCTCTTCn'nnn_

Cuts at:      0     155     518    1417    2371    7416 
   Size:        155     363     899     954    5045

  Fragments arranged by size: 

                5045     954     899     363     155
 
SbfI CC_TGCA'GG

Cuts at:      0    3932    7416 
   Size:       3932    3484
 
ScaI AGT'ACT

Cuts at:      0     460    2683    7416 
   Size:        460    2223    4733

  Fragments arranged by size: 

                4733    2223     460
 
SexAI A'CCwGG_T

Cuts at:      0    6502    7416 
   Size:       6502     914
 
SfcI C'TryA_G

Cuts at:      0     436     733    1256    1405    1723    2570    2668    3307 
   Size:        436     297     523     149     318     847      98     639

Cuts at:   3307    3652    3928    4021    4339    4510    5891    6073    6544 
   Size:        345     276      93     318     171    1381     182     471

Cuts at:   6544    6577    7416 
   Size:         33     839

  Fragments arranged by size: 

                1381     847     839     639     523     471     436     345
                 318     318     297     276     182     171     149      98
                  93      33
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    6163    7416 
   Size:       6163    1253
 
SmaI CCC'GGG

Cuts at:      0    3094    4390    5584    7303    7416 
   Size:       3094    1296    1194    1719     113

  Fragments arranged by size: 

                3094    1719    1296    1194     113
 
SmlI C'TyrA_G

Cuts at:      0    1516    1753    2305    7416 
   Size:       1516     237     552    5111

  Fragments arranged by size: 

                5111    1516     552     237
 
SphI G_CATG'C

Cuts at:      0    4801    6906    6912    7416 
   Size:       4801    2105       6     504

  Fragments arranged by size: 

                4801    2105     504       6
 
StuI AGG'CCT

Cuts at:      0    3958    4297    4396    4516    6079    7416 
   Size:       3958     339      99     120    1563    1337

  Fragments arranged by size: 

                3958    1563    1337     339     120      99
 
StyI C'CwwG_G

Cuts at:      0    1043    1493    1889    1934    2315    2546    3873    4103 
   Size:       1043     450     396      45     381     231    1327     230

Cuts at:   4103    4553    5606    6152    6365    6512    6812    7079    7274 
   Size:        450    1053     546     213     147     300     267     195

Cuts at:   7274    7416 
   Size:        142

  Fragments arranged by size: 

                1327    1053    1043     546     450     450     396     381
                 300     267     231     230     213     195     147     142
                  45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      86    1116    2210    3226    3994    7416 
   Size:         86    1030    1094    1016     768    3422

  Fragments arranged by size: 

                3422    1094    1030    1016     768      86
 
TatI w'GTAC_w

Cuts at:      0     458    1830    2681    3702    6842    7416 
   Size:        458    1372     851    1021    3140     574

  Fragments arranged by size: 

                3140    1372    1021     851     574     458
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    6133    6165    7119    7151    7416 
   Size:       6133      32     954      32     265

  Fragments arranged by size: 

                6133     954     265      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     928    7416 
   Size:        928    6488
 
XbaI T'CTAG_A

Cuts at:      0     918    7416 
   Size:        918    6498
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     300    1941    6519    7416 
   Size:        300    1641    4578     897

  Fragments arranged by size: 

                4578    1641     897     300
 
XmaI C'CCGG_G

Cuts at:      0    3092    4388    5582    7301    7416 
   Size:       3092    1296    1194    1719     115

  Fragments arranged by size: 

                3092    1719    1296    1194     115
 
XmnI GAAnn'nnTTC

Cuts at:      0     188     578    1511    7416 
   Size:        188     390     933    5905

  Fragments arranged by size: 

                5905     933     390     188
 
ZraI GAC'GTC

Cuts at:      0    4603    7416 
   Size:       4603    2813
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NheI     NspI     PasI    PflMI     PfoI    PpuMI
     PsiI   PspOMI     PstI     PvuI    PvuII     SacI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AgeI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI    BfrBI    BspEI    BsrGI   BssHII    BstBI
  BstZ17I    CspCI     DraI     FseI     HpaI     KasI     KpnI     MluI
     NarI     NdeI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI    PspXI     PsrI    RsrII    SacII     SalI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     XhoI
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