Restriction map for pF1KB5406
 (Linear) (Six-base) MAPSORT of: KIBB5406.seq  Check: 1975  from: 1  to: 993



KIBB5406.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     156     993 
   Size:        156     837
 
AccI GT'mk_AC

Cuts at:      0      85     993 
   Size:         85     908
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     538     993 
   Size:          2     536     455

  Fragments arranged by size: 

                 536     455       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     963     993 
   Size:        963      30
 
AseI AT'TA_AT

Cuts at:      0     433     993 
   Size:        433     560
 
BanI G'GyrC_C

Cuts at:      0     595     993 
   Size:        595     398
 
BglII A'GATC_T

Cuts at:      0     314     993 
   Size:        314     679
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     874     993 
   Size:        349     525     119

  Fragments arranged by size: 

                 525     349     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     814     993 
   Size:         38     776     179

  Fragments arranged by size: 

                 776     179      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52     850     993 
   Size:         52     798     143

  Fragments arranged by size: 

                 798     143      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     706     736     993 
   Size:        706      30     257

  Fragments arranged by size: 

                 706     257      30
 
BseYI C'CCAG_C

Cuts at:      0      30     993 
   Size:         30     963
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     993 
   Size:        268     725
 
BsiHKAI G_wGCw'C

Cuts at:      0     971     993 
   Size:        971      22
 
BsmI GAATG_Cn'

Cuts at:      0     512     993 
   Size:        512     481
 
Bsp1286I G_dGCh'C

Cuts at:      0     971     993 
   Size:        971      22
 
BspHI T'CATG_A

Cuts at:      0      95     993 
   Size:         95     898
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     156     848     993 
   Size:        156     692     145

  Fragments arranged by size: 

                 692     156     145
 
BsrGI T'GTAC_A

Cuts at:      0     491     660     993 
   Size:        491     169     333

  Fragments arranged by size: 

                 491     333     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     576     993 
   Size:        576     417
 
BstYI r'GATC_y

Cuts at:      0     314     446     993 
   Size:        314     132     547

  Fragments arranged by size: 

                 547     314     132
 
BstZ17I GTA'TAC

Cuts at:      0      86     993 
   Size:         86     907
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      91     993 
   Size:         91     902
 
ClaI AT'CG_AT

Cuts at:      0     804     993 
   Size:        804     189
 
DraI TTT'AAA

Cuts at:      0     378     993 
   Size:        378     615
 
EarI CTCTTCn'nnn_

Cuts at:      0     432     575     993 
   Size:        432     143     418

  Fragments arranged by size: 

                 432     418     143
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     349     538     874     993 
   Size:          2     347     189     336     119

  Fragments arranged by size: 

                 347     336     189     119       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     960     993 
   Size:        960      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     409     441     460     492     550     582     706     738 
   Size:        409      32      19      32      58      32     124      32

Cuts at:    738     993 
   Size:        255

  Fragments arranged by size: 

                 409     255     124      58      32      32      32      32
                  19
 
HindIII A'AGCT_T

Cuts at:      0     176     993 
   Size:        176     817
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     993 
   Size:         95     898
 
MspA1I CmG'CkG

Cuts at:      0      34     993 
   Size:         34     959
 
NspI r_CATG'y

Cuts at:      0     255     993 
   Size:        255     738
 
PfoI T'CCnGG_A

Cuts at:      0     365     993 
   Size:        365     628
 
PstI C_TGCA'G

Cuts at:      0     167     959     993 
   Size:        167     792      34

  Fragments arranged by size: 

                 792     167      34
 
PvuII CAG'CTG

Cuts at:      0      34     993 
   Size:         34     959
 
SapI GCTCTTCn'nnn_

Cuts at:      0     575     993 
   Size:        575     418
 
SbfI CC_TGCA'GG

Cuts at:      0     167     959     993 
   Size:        167     792      34

  Fragments arranged by size: 

                 792     167      34
 
SfcI C'TryA_G

Cuts at:      0     163     586     955     993 
   Size:        163     423     369      38

  Fragments arranged by size: 

                 423     369     163      38
 
SmlI C'TyrA_G

Cuts at:      0      53     829     993 
   Size:         53     776     164

  Fragments arranged by size: 

                 776     164      53
 
SphI G_CATG'C

Cuts at:      0     255     993 
   Size:        255     738
 
SspI AAT'ATT

Cuts at:      0     609     993 
   Size:        609     384
 
StyI C'CwwG_G

Cuts at:      0     305     993 
   Size:        305     688
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     517     993 
   Size:        517     476
 
TatI w'GTAC_w

Cuts at:      0     491     660     993 
   Size:        491     169     333

  Fragments arranged by size: 

                 491     333     169
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     612     993 
   Size:        612     381
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI     AseI     BanI    BglII     BpmI
    BpuEI     BsaI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrGI    BstXI    BstYI  BstZ17I    BtgZI     ClaI
     DraI     EarI  Eco57MI EcoO109I    Hin4I  HindIII     MmeI   MspA1I
     NspI     PfoI     PstI    PvuII     SapI     SbfI     SfcI     SmlI
     SphI     SspI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I     BtgI     BtsI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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