Restriction map for pF1KB5377
 (Linear) (Six-base) MAPSORT of: KIBB5377.seq  Check: 6786  from: 1  to: 1020



KIBB5377.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

Acc65I G'GTAC_C

Cuts at:      0     259    1020 
   Size:        259     761
 
AleI CACnn'nnGTG

Cuts at:      0     427    1020 
   Size:        427     593
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84     533    1020 
   Size:         84     449     487

  Fragments arranged by size: 

                 487     449      84
 
AvaI C'yCGr_G

Cuts at:      0     922    1020 
   Size:        922      98
 
BanI G'GyrC_C

Cuts at:      0     118     250     259    1020 
   Size:        118     132       9     761

  Fragments arranged by size: 

                 761     132     118       9
 
BanII G_rGCy'C

Cuts at:      0     116     464    1020 
   Size:        116     348     556

  Fragments arranged by size: 

                 556     348     116
 
BbeI G_GCGC'C

Cuts at:      0     122    1020 
   Size:        122     898
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     605    1020 
   Size:        605     415
 
BbvCI CC'TCA_GC

Cuts at:      0     146    1020 
   Size:        146     874
 
BglI GCCn_nnn'nGGC

Cuts at:      0     233    1020 
   Size:        233     787
 
BglII A'GATC_T

Cuts at:      0     185     521    1020 
   Size:        185     336     499

  Fragments arranged by size: 

                 499     336     185
 
BlpI GC'TnA_GC

Cuts at:      0     163    1020 
   Size:        163     857
 
BmgBI CAC'GTC

Cuts at:      0     159    1020 
   Size:        159     861
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224     481    1020 
   Size:        224     257     539

  Fragments arranged by size: 

                 539     257     224
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     919    1020 
   Size:        919     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     914    1020 
   Size:        914     106
 
Bpu10I CC'TnA_GC

Cuts at:      0     146    1020 
   Size:        146     874
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     281     665    1020 
   Size:        281     384     355

  Fragments arranged by size: 

                 384     355     281
 
BsaBI GATnn'nnATC

Cuts at:      0     550    1020 
   Size:        550     470
 
BsaHI Gr'CG_yC

Cuts at:      0     119    1020 
   Size:        119     901
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     664     694     781     811    1020 
   Size:        664      30      87      30     209

  Fragments arranged by size: 

                 664     209      87      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     139    1020 
   Size:         67      72     881

  Fragments arranged by size: 

                 881      72      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     789    1020 
   Size:        789     231
 
BsiEI CG_ry'CG

Cuts at:      0     195    1020 
   Size:        195     825
 
BsiHKAI G_wGCw'C

Cuts at:      0     164    1020 
   Size:        164     856
 
BsmI GAATG_Cn'

Cuts at:      0     587    1020 
   Size:        587     433
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     164     464    1020 
   Size:        116      48     300     556

  Fragments arranged by size: 

                 556     300     116      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     996    1020 
   Size:        996      24
 
BsrBI CCG'CTC

Cuts at:      0     443     449    1020 
   Size:        443       6     571

  Fragments arranged by size: 

                 571     443       6
 
BstBI TT'CG_AA

Cuts at:      0     591    1020 
   Size:        591     429
 
BstYI r'GATC_y

Cuts at:      0     185     480     521    1020 
   Size:        185     295      41     499

  Fragments arranged by size: 

                 499     295     185      41
 
BtgI C'CryG_G

Cuts at:      0      36     282    1020 
   Size:         36     246     738

  Fragments arranged by size: 

                 738     246      36
 
DraI TTT'AAA

Cuts at:      0     716    1020 
   Size:        716     304
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     505    1020 
   Size:        505     515
 
EaeI y'GGCC_r

Cuts at:      0     438    1020 
   Size:        438     582
 
EarI CTCTTCn'nnn_

Cuts at:      0     512    1020 
   Size:        512     508
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     914    1020 
   Size:        914     106
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     787     896    1020 
   Size:        787     109     124

  Fragments arranged by size: 

                 787     124     109
 
EcoO109I rG'GnC_Cy

Cuts at:      0     936    1020 
   Size:        936      84
 
EcoRV GAT'ATC

Cuts at:      0     702    1020 
   Size:        702     318
 
HaeII r_GCGC'y

Cuts at:      0      85     122    1020 
   Size:         85      37     898

  Fragments arranged by size: 

                 898      85      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     664     696    1020 
   Size:        376      32     256      32     324

  Fragments arranged by size: 

                 376     324     256      32      32
 
KasI G'GCGC_C

Cuts at:      0     118    1020 
   Size:        118     902
 
KpnI G_GTAC'C

Cuts at:      0     263    1020 
   Size:        263     757
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     707    1020 
   Size:         26     681     313

  Fragments arranged by size: 

                 681     313      26
 
MslI CAynn'nnrTG

Cuts at:      0     427     619    1020 
   Size:        427     192     401

  Fragments arranged by size: 

                 427     401     192
 
MspA1I CmG'CkG

Cuts at:      0      38     354     530     697    1020 
   Size:         38     316     176     167     323

  Fragments arranged by size: 

                 323     316     176     167      38
 
NarI GG'CG_CC

Cuts at:      0     119    1020 
   Size:        119     901
 
NcoI C'CATG_G

Cuts at:      0     282    1020 
   Size:        282     738
 
NspI r_CATG'y

Cuts at:      0     879    1020 
   Size:        879     141
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      24     394    1020 
   Size:         24     370     626

  Fragments arranged by size: 

                 626     370      24
 
PfoI T'CCnGG_A

Cuts at:      0     785     891    1020 
   Size:        785     106     129

  Fragments arranged by size: 

                 785     129     106
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147    1020 
   Size:        115      32     873

  Fragments arranged by size: 

                 873     115      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     936    1020 
   Size:        936      84
 
PvuII CAG'CTG

Cuts at:      0     697    1020 
   Size:        697     323
 
SacII CC_GC'GG

Cuts at:      0      39    1020 
   Size:         39     981
 
SfcI C'TryA_G

Cuts at:      0     525    1020 
   Size:        525     495
 
SfoI GGC'GCC

Cuts at:      0     120    1020 
   Size:        120     900
 
SmlI C'TyrA_G

Cuts at:      0    1000    1020 
   Size:       1000      20
 
SphI G_CATG'C

Cuts at:      0     879    1020 
   Size:        879     141
 
SspI AAT'ATT

Cuts at:      0     608    1020 
   Size:        608     412
 
StuI AGG'CCT

Cuts at:      0     151     212     870     902    1020 
   Size:        151      61     658      32     118

  Fragments arranged by size: 

                 658     151     118      61      32
 
StyI C'CwwG_G

Cuts at:      0     282     662    1020 
   Size:        282     380     358

  Fragments arranged by size: 

                 380     358     282
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     403     508    1020 
   Size:        403     105     512

  Fragments arranged by size: 

                 512     403     105
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     781     813    1020 
   Size:        781      32     207

  Fragments arranged by size: 

                 781     207      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     974    1020 
   Size:        974      46
 

 Enzymes that do cut:

   Acc65I     AleI    AlwNI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BglI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BstBI    BstYI     BtgI
     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
    HaeII    Hin4I     KasI     KpnI     MmeI     MslI   MspA1I     NarI
     NcoI     NspI    PflMI     PfoI     PpiI    PpuMI    PvuII    SacII
     SfcI     SfoI     SmlI     SphI     SspI     StuI     StyI    TaqII
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI
    BfrBI Bme1580I     BmtI    BpuEI    BsaAI    BsaWI    BseYI    BsiWI
    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     EagI     EciI  EcoICRI    EcoRI     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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