Restriction map for pF1KB5344
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BbrPI       BbvCI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BpmI        BsaAI       BsaJI       BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstXI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HapII       HgaI        HincII      HindII      HindIII     
HpaI        HpaII       KpnI        KspI        MaeIII      
MfeI        MluI        MroI        MseI        MslI        
MspI        MunI        NaeI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   68 enzymes) #
###################################

AcyI          432  GGCGCC               G[GA]^CG[CT]C
AfaI          401  GTAC                 GT^AC
AlwI          208  GGATC                GGATC(4/5)
AocI          203  CCTCAGG              CC^T[ACGT]AGG
ApaI           14  GGGCCC               GGGCC^C
BalI           32  TGGCCA               TGG^CCA
BanI          432  GGCGCC               G^G[CT][GA]CC
BbeI          432  GGCGCC               GGCGC^C
BbsI           75  GAAGAC               GAAGAC(2/6)
BcgI          249  CGACTCGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         198  GGATAC               GTATCC(6/5)
BlpI          500  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      500  GCTCAGC              GC^T[ACGT]AGC
BpuAI          75  GAAGAC               GAAGAC(2/6)
BsaBI         106  GATTGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         432  GGCGCC               G[GA]^CG[CT]C
BsaI          118  GAGACC               GGTCTC(1/5)
BsiYI         423  CCAGAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          423  CCAGAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI           9  GAGACG               CGTCTC(1/5)
BspMI         383  ACCTGC               ACCTGC(4/8)
BssKI         492  CCTGG                ^CC[ACGT]GG
BstBI         139  TTCGAA               TT^CGAA
BstNI         492  CCTGG                CC^[AT]GG
BstYI         208  GGATCT               [GA]^GATC[CT]
Bsu36I        203  CCTCAGG              CC^T[ACGT]AGG
Cac8I         429  GCAGGC               GC[ACGT]^[ACGT]GC
CelII         500  GCTCAGC              GC^T[ACGT]AGC
DpnII         209  GATC                 ^GATC
DraII          14  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI           32  TGGCCA               [CT]^GGCC[GA]
Eco81I        203  CCTCAGG              CC^T[ACGT]AGG
EcoO109I       14  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        492  CCTGG                ^CC[AT]GG
FauI          353  CCCGC                CCCGC(4/6)
HaeII         432  GGCGCC               [GA]GCGC^[CT]
Hin1I         432  GGCGCC               G[GA]^CG[CT]C
HinfI         250  GACTC                G^A[ACGT]TC
HphI          509  TCACC                GGTGA(8/7)
KasI          432  GGCGCC               G^GCGCC
MaeII          62  ACGT                 A^CGT
MamI          106  GATTGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          209  GATC                 ^GATC
MflI          208  GGATCT               [GA]^GATC[CT]
MluNI          32  TGGCCA               TGG^CCA
MscI           32  TGGCCA               TGG^CCA
MspA1I        472  CAGCTG               C[AC]G^C[GT]G
MvaI          492  CCTGG                CC^[AT]GG
MwoI          524  GCATCCACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI          432  GGCGCC               GG^CGCC
NdeII         209  GATC                 ^GATC
NlaIII        552  CATG                 CATG^
NspV          139  TTCGAA               TT^CGAA
PleI          250  GACTC                GAGTC(4/5)
PvuII         472  CAGCTG               CAG^CTG
RsaI          401  GTAC                 GT^AC
SapI          588  GAAGAGC              GCTCTTC(1/4)
Sau3AI        209  GATC                 ^GATC
ScaI          400  AGTACT               AGT^ACT
ScrFI         492  CCTGG                CC^[ACGT]GG
SfcI          243  CTACAG               C^T[GA][CT]AG
SfoI          432  GGCGCC               GGC^GCC
SfuI          139  TTCGAA               TT^CGAA
SmlI          310  CTCAAG               C^T[CT][GA]AG
TaqI          140  TCGA                 T^CGA
Tsp509I       145  AATT                 ^AATT
TthHB8I       140  TCGA                 T^CGA
XhoII         208  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII          81  CGCG                 CG^CG
              355  CGCG                
AciI           80  CCGC                 CCGC(-3/-1)
              354  CCGC                
BanII          14  GGGCCC               G[GA]GC[CT]^C
              591  GAGCCC              
BbvI          471  GCAGC                GCAGC(8/12)
              371  GCTGC               
BfaI          334  CTAG                 C^TAG
              572  CTAG                
BsgI           64  GTGCAG               GTGCAG(16/14)
              479  GTGCAG              
BsrI           25  CCAGT                ACTGG(1/-1)
              632  CCAGT               
BstUI          81  CGCG                 CG^CG
              355  CGCG                
CfoI          305  GCGC                 GCG^C
              433  GCGC                
Cfr13I         14  GGGCC                G^G[ACGT]CC
               15  GGCCC               
EarI          651  CTCTTC               CTCTTC(1/4)
              588  GAAGAG              
Fnu4HI        371  GCTGC                GC^[ACGT]GC
              471  GCAGC               
HhaI          305  GCGC                 GCG^C
              433  GCGC                
HinP1I        305  GCGC                 G^CGC
              433  GCGC                
ItaI          371  GCTGC                GC^[ACGT]GC
              471  GCAGC               
Ksp632I       651  CTCTTC               CTCTTC(1/4)
              588  GAAGAG              
MaeI          334  CTAG                 C^TAG
              572  CTAG                
MvnI           81  CGCG                 CG^CG
              355  CGCG                
Sau96I         14  GGGCC                G^G[ACGT]CC
               15  GGCCC               
TseI          371  GCTGC                G^C[AT]GC
              471  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BmyI        BsmAI       BsmFI       Bsp1286I    
BstF5I      DdeI        FokI        HaeIII      MboII       
MnlI        NlaIV       SfaNI       TspRI       
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