Restriction map for pF1KB5344
 (Linear) (Six-base) MAPSORT of: KIBB5344.seq  Check: 8585  from: 1  to: 696



KIBB5344.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     473     494     696 
   Size:        473      21     202

  Fragments arranged by size: 

                 473     202      21
 
ApaI G_GGCC'C

Cuts at:      0      18     696 
   Size:         18     678
 
BanI G'GyrC_C

Cuts at:      0     432     696 
   Size:        432     264
 
BanII G_rGCy'C

Cuts at:      0      18     595     696 
   Size:         18     577     101

  Fragments arranged by size: 

                 577     101      18
 
BbeI G_GCGC'C

Cuts at:      0     436     696 
   Size:        436     260
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      82     696 
   Size:         82     614
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     238     272     696 
   Size:        238      34     424

  Fragments arranged by size: 

                 424     238      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     192     696 
   Size:        192     504
 
BlpI GC'TnA_GC

Cuts at:      0     501     696 
   Size:        501     195
 
Bme1580I G_kGCm'C

Cuts at:      0      18     131     696 
   Size:         18     113     565

  Fragments arranged by size: 

                 565     113      18
 
BmgBI CAC'GTC

Cuts at:      0      63     696 
   Size:         63     633
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     626     696 
   Size:        626      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     696 
   Size:        295     401
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     112     696 
   Size:        112     584
 
BsaBI GATnn'nnATC

Cuts at:      0     110     696 
   Size:        110     586
 
BsaHI Gr'CG_yC

Cuts at:      0     433     696 
   Size:        433     263
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     605     635     696 
   Size:        605      30      61

  Fragments arranged by size: 

                 605      61      30
 
BseYI C'CCAG_C

Cuts at:      0      20     346     474     696 
   Size:         20     326     128     222

  Fragments arranged by size: 

                 326     222     128      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     500     696 
   Size:         85     415     196

  Fragments arranged by size: 

                 415     196      85
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       3     696 
   Size:          3     693
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     131     595     696 
   Size:         18     113     464     101

  Fragments arranged by size: 

                 464     113     101      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     392     696 
   Size:        392     304
 
BstBI TT'CG_AA

Cuts at:      0     140     696 
   Size:        140     556
 
BstYI r'GATC_y

Cuts at:      0     208     696 
   Size:        208     488
 
Bsu36I CC'TnA_GG

Cuts at:      0     204     696 
   Size:        204     492
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      97     696 
   Size:         97     599
 
BtsI GCAGTG_nn'

Cuts at:      0      73     577     696 
   Size:         73     504     119

  Fragments arranged by size: 

                 504     119      73
 
EaeI y'GGCC_r

Cuts at:      0      32     696 
   Size:         32     664
 
EarI CTCTTCn'nnn_

Cuts at:      0     583     657     696 
   Size:        583      74      39

  Fragments arranged by size: 

                 583      74      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     696 
   Size:         15     681
 
HaeII r_GCGC'y

Cuts at:      0     436     696 
   Size:        436     260
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29      61     696 
   Size:         29      32     635

  Fragments arranged by size: 

                 635      32      29
 
KasI G'GCGC_C

Cuts at:      0     432     696 
   Size:        432     264
 
MscI TGG'CCA

Cuts at:      0      34     696 
   Size:         34     662
 
MspA1I CmG'CkG

Cuts at:      0     474     696 
   Size:        474     222
 
NarI GG'CG_CC

Cuts at:      0     433     696 
   Size:        433     263
 
PspOMI G'GGCC_C

Cuts at:      0      14     696 
   Size:         14     682
 
PvuII CAG'CTG

Cuts at:      0     474     696 
   Size:        474     222
 
SapI GCTCTTCn'nnn_

Cuts at:      0     583     696 
   Size:        583     113
 
ScaI AGT'ACT

Cuts at:      0     402     696 
   Size:        402     294
 
SfcI C'TryA_G

Cuts at:      0     243     696 
   Size:        243     453
 
SfoI GGC'GCC

Cuts at:      0     434     696 
   Size:        434     262
 
SmlI C'TyrA_G

Cuts at:      0     310     696 
   Size:        310     386
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     466     696 
   Size:        466     230
 
TatI w'GTAC_w

Cuts at:      0     400     696 
   Size:        400     296
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      52      84     696 
   Size:         52      32     612

  Fragments arranged by size: 

                 612      52      32
 

 Enzymes that do cut:

     AlfI     ApaI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BlpI Bme1580I    BmgBI     BmrI    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsmBI Bsp1286I    BspMI    BstBI    BstYI
   Bsu36I    BtgZI     BtsI     EaeI     EarI EcoO109I    HaeII    Hin4I
     KasI     MscI   MspA1I     NarI   PspOMI    PvuII     SapI     ScaI
     SfcI     SfoI     SmlI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI
     BclI    BfrBI     BglI    BglII     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com