Restriction map for pF1KB5332
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaBI       BsaHI       BseAI       BsiEI       BsmI        
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MluI        MroI        
MspA1I      MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII         335  CGCG                 CG^CG
AcsI          184  GAATTC               [GA]^AATT[CT]
AlwNI         469  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          573  CCTGAGG              CC^T[ACGT]AGG
ApoI          184  GAATTC               [GA]^AATT[CT]
AseI          207  ATTAAT               AT^TAAT
AsnI          207  ATTAAT               AT^TAAT
BanII          37  GAGCTC               G[GA]GC[CT]^C
BbvCI         118  GCTGAGG              CCTCAGC(-5/-2)
BbvI          243  GCTGC                GCAGC(8/12)
BcgI          246  GCAGTACTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI         424  TACGTG               [CT]AC^GT[GA]
BsaI          353  GAGACC               GGTCTC(1/5)
BsaWI         384  TCCGGT               [AT]^CCGG[AT]
BseRI         271  GAGGAG               GAGGAG(10/8)
BsgI          261  CTGCAC               GTGCAG(16/14)
BsiWI           5  CGTACG               C^GTACG
BsmBI         347  CGTCTC               CGTCTC(1/5)
BsmFI         257  GGGAC                GGGAC(10/14)
BspHI         177  TCATGA               T^CATGA
BsrI          143  ACTGG                ACTGG(1/-1)
BssSI         793  CACGAG               CACGAG(-5/-1)
BstUI         335  CGCG                 CG^CG
BstYI         797  AGATCC               [GA]^GATC[CT]
Bsu36I        573  CCTGAGG              CC^T[ACGT]AGG
Cfr13I        465  GGGCC                G^G[ACGT]CC
DraIII        580  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           46  GACGAGAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        573  CCTGAGG              CC^T[ACGT]AGG
EcoNI         532  CCTACTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         184  GAATTC               G^AATTC
Fnu4HI        243  GCTGC                GC^[ACGT]GC
ItaI          243  GCTGC                GC^[ACGT]GC
MflI          797  AGATCC               [GA]^GATC[CT]
MslI          381  CATTCCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          335  CGCG                 CG^CG
PaeR7I        350  CTCGAG               C^TCGAG
PflMI         137  CCATTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         207  ATTAAT               AT^TAAT
RcaI          177  TCATGA               T^CATGA
SacI           37  GAGCTC               GAGCT^C
Sau96I        465  GGGCC                G^G[ACGT]CC
SfaNI         157  GATGC                GCATC(5/9)
SmaI          374  CCCGGG               CCC^GGG
SmlI          350  CTCGAG               C^T[CT][GA]AG
SphI          516  GCATGC               GCATG^C
TfiI           99  GATTC                G^A[AT]TC
TseI          243  GCTGC                G^C[AT]GC
Van91I        137  CCATTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          416  CCAATGACTACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          350  CTCGAG               C^TCGAG
XhoII         797  AGATCC               [GA]^GATC[CT]
XmaCI         374  CCCGGG               C^CCGGG
XmaI          374  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        265  ACATGT               A^C[GA][CT]GT
              339  ACATGT              
AspHI          37  GAGCTC               G[AT]GC[AT]^C
              288  GTGCTC              
BalI           89  TGGCCA               TGG^CCA
              607  TGGCCA              
BbsI          325  GAAGAC               GAAGAC(2/6)
              109  GTCTTC              
BmyI           37  GAGCTC               G[AGT]GC[ACT]^C
              288  GTGCTC              
BpuAI         325  GAAGAC               GAAGAC(2/6)
              109  GTCTTC              
BsiHKAI        37  GAGCTC               G[AT]GC[AT]^C
              288  GTGCTC              
Bsp1286I       37  GAGCTC               G[AGT]GC[ACT]^C
              288  GTGCTC              
BspLU11I      265  ACATGT               A^CATGT
              339  ACATGT              
BsrDI         189  CATTGC               GCAATG(2/0)
              320  GCAATG              
BstNI          81  CCTGG                CC^[AT]GG
              468  CCAGG               
EaeI           89  TGGCCA               [CT]^GGCC[GA]
              607  TGGCCA              
EcoRII         81  CCTGG                ^CC[AT]GG
              468  CCAGG               
HgaI          333  GACGC                GACGC(5/10)
              717  GCGTC               
HphI          387  GGTGA                GGTGA(8/7)
              302  TCACC               
MaeII         346  ACGT                 A^CGT
              425  ACGT                
MaeIII        343  GTCAC                ^GT[ACGT]AC
              388  GTGAC               
MluNI          89  TGGCCA               TGG^CCA
              607  TGGCCA              
MscI           89  TGGCCA               TGG^CCA
              607  TGGCCA              
MseI          208  TTAA                 T^TAA
              787  TTAA                
MvaI           81  CCTGG                CC^[AT]GG
              468  CCAGG               
PleI          643  GAGTC                GAGTC(4/5)
              700  GAGTC               
PstI          244  CTGCAG               CTGCA^G
              536  CTGCAG              
ScaI          248  AGTACT               AGT^ACT
              620  AGTACT              
SfcI          244  CTGCAG               C^T[GA][CT]AG
              536  CTGCAG              
Tru9I         208  TTAA                 T^TAA
              787  TTAA                
Tsp45I        343  GTCAC                ^GT[GC]AC
              388  GTGAC               
TspRI          42  CACTG                CA[GC]TG[ACGT][ACGT]^
              584  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BsoBI       BssKI       BstF5I      Cac8I       DdeI        
DpnII       FokI        HaeIII      HapII       HinfI       
HpaII       MboI        MboII       MnlI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      ScrFI       TaqI        
Tsp509I     TthHB8I     
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