Restriction map for pF1KB5225
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       BamHI       BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BsaAI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstUI       BstYI       BstZ17I     
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KspI        MfeI        MflI        
MluI        MroI        MseI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1199  GGTACC               G^GTACC
AcsI          492  GAATTC               [GA]^AATT[CT]
AflIII       1427  ACGTGT               A^C[GA][CT]GT
AlwI         1383  GATCC                GGATC(4/5)
ApaI         1133  GGGCCC               GGGCC^C
ApoI          492  GAATTC               [GA]^AATT[CT]
Asp718I      1199  GGTACC               G^GTACC
AspHI         726  GAGCTC               G[AT]GC[AT]^C
AvrII         513  CCTAGG               C^CTAGG
BbsI         1347  GTCTTC               GAAGAC(2/6)
BfaI          514  CTAG                 C^TAG
BlnI          513  CCTAGG               C^CTAGG
BlpI          748  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      748  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1347  GTCTTC               GAAGAC(2/6)
BsaBI         706  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       726  GAGCTC               G[AT]GC[AT]^C
BspHI         890  TCATGA               T^CATGA
BsrDI         609  GCAATG               GCAATG(2/0)
BssSI         442  CTCGTG               CACGAG(-5/-1)
BstAPI         18  GCACCGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1638  GGTCACC              G^GT[ACGT]ACC
BstPI        1638  GGTCACC              G^GT[ACGT]ACC
CelII         748  GCTGAGC              GC^T[ACGT]AGC
DpnII        1383  GATC                 ^GATC
DraIII       1024  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1638  GGTCACC              G^GT[ACGT]ACC
EcoRI         492  GAATTC               G^AATTC
HindIII       211  AAGCTT               A^AGCTT
KpnI         1199  GGTACC               GGTAC^C
MaeI          514  CTAG                 C^TAG
MamI          706  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI         1383  GATC                 ^GATC
NcoI          548  CCATGG               C^CATGG
NdeII        1383  GATC                 ^GATC
NspI           23  GCATGC               [GA]CATG^[CT]
RcaI          890  TCATGA               T^CATGA
SacI          726  GAGCTC               GAGCT^C
Sau3AI       1383  GATC                 ^GATC
ScaI          623  AGTACT               AGT^ACT
SmaI          525  CCCGGG               CCC^GGG
SphI           23  GCATGC               GCATG^C
Tsp509I       493  AATT                 ^AATT
XcmI         1352  CCATCCAGGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         525  CCCGGG               C^CCGGG
XmaI          525  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AatII         364  GACGTC               GACGT^C
             1820  GACGTC              
AlwNI         109  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1601  CAGAGTCTG           
AocI         1194  CCTCAGG              CC^T[ACGT]AGG
             2023  CCTGAGG             
AspI         1462  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1599  GACAGAGTC           
AvaI          525  CCCGGG               C^[CT]CG[GA]G
             1177  CCCGAG              
BalI          551  TGGCCA               TGG^CCA
             1297  TGGCCA              
BbeI         1704  GGCGCC               GGCGC^C
             1895  GGCGCC              
BsoBI         525  CCCGGG               C^[CT]CG[GA]G
             1177  CCCGAG              
BsrBI        1749  CCGCTC               CCGCTC(-3/-3)
              180  GAGCGG              
BsrFI         120  GCCGGC               [GA]^CCGG[CT]
             1904  GCCGGC              
Bsu36I       1194  CCTCAGG              CC^T[ACGT]AGG
             2023  CCTGAGG             
Cfr10I        120  GCCGGC               [GA]^CCGG[CT]
             1904  GCCGGC              
DsaI          548  CCATGG               C^C[GA][CT]GG
              765  CCACGG              
Eco81I       1194  CCTCAGG              CC^T[ACGT]AGG
             2023  CCTGAGG             
KasI         1704  GGCGCC               G^GCGCC
             1895  GGCGCC              
MluNI         551  TGGCCA               TGG^CCA
             1297  TGGCCA              
MscI          551  TGGCCA               TGG^CCA
             1297  TGGCCA              
NaeI          120  GCCGGC               GCC^GGC
             1904  GCCGGC              
NarI         1704  GGCGCC               GG^CGCC
             1895  GGCGCC              
NgoMIV        120  GCCGGC               G^CCGGC
             1904  GCCGGC              
PflFI        1462  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1599  GACAGAGTC           
PflMI         993  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1356  CCAGGTGGTGG         
SfoI         1704  GGCGCC               GGC^GCC
             1895  GGCGCC              
Tth111I      1462  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1599  GACAGAGTC           
Van91I        993  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1356  CCAGGTGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AvaII       
BanI        BanII       BbvCI       BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmBI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      TspRI       
TthHB8I     
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