Restriction map for pF1KB5169
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsgI        BsiEI       BsiWI       BsmBI       BspDI       
BspHI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KspI        MamI        MfeI        MflI        MluI        
MspA1I      MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoII       XmnI        


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# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        592  GGTACC               G^GTACC
AccI          403  GTCTAC               GT^[AC][GT]AC
AcyI         1091  GGCGCC               G[GA]^CG[CT]C
AflIII       1235  ACATGT               A^C[GA][CT]GT
AlwNI           7  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          893  GGGCCC               GGGCC^C
Asp718I       592  GGTACC               G^GTACC
BalI         1191  TGGCCA               TGG^CCA
BbeI         1091  GGCGCC               GGCGC^C
BfaI          574  CTAG                 C^TAG
BglI          564  GCCCCGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1091  GGCGCC               G[GA]^CG[CT]C
BsmI          627  GCATTC               GAATGC(1/-1)
Bsp1407I     1238  TGTACA               T^GTACA
BspLU11I     1235  ACATGT               A^CATGT
BspMI          62  ACCTGC               ACCTGC(4/8)
BsrDI         120  CATTGC               GCAATG(2/0)
BsrFI         782  ACCGGC               [GA]^CCGG[CT]
BsrGI        1238  TGTACA               T^GTACA
BsrI            6  CCAGT                ACTGG(1/-1)
BstXI          20  CCAACCTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        782  ACCGGC               [GA]^CCGG[CT]
DsaI          429  CCGTGG               C^C[GA][CT]GG
EaeI         1191  TGGCCA               [CT]^GGCC[GA]
Hin1I        1091  GGCGCC               G[GA]^CG[CT]C
HphI          967  GGTGA                GGTGA(8/7)
KasI         1091  GGCGCC               G^GCGCC
KpnI          592  GGTACC               GGTAC^C
MaeI          574  CTAG                 C^TAG
MaeII         530  ACGT                 A^CGT
MluNI        1191  TGGCCA               TGG^CCA
MscI         1191  TGGCCA               TGG^CCA
MseI          257  TTAA                 T^TAA
NarI         1091  GGCGCC               GG^CGCC
NruI          804  TCGCGA               TCG^CGA
PaeR7I        152  CTCGAG               C^TCGAG
SacI         1000  GAGCTC               GAGCT^C
SfcI          405  CTACAG               C^T[GA][CT]AG
SfoI         1091  GGCGCC               GGC^GCC
SmaI          776  CCCGGG               CCC^GGG
SmlI          152  CTCGAG               C^T[CT][GA]AG
SspBI        1238  TGTACA               T^GTACA
StuI          582  AGGCCT               AGG^CCT
TfiI          346  GAATC                G^A[AT]TC
Tru9I         257  TTAA                 T^TAA
Tsp509I       331  AATT                 ^AATT
TspRI        1152  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          152  CTCGAG               C^TCGAG
XmaCI         776  CCCGGG               C^CCGGG
XmaI          776  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccIII        356  TCCGGA               T^CCGGA
             1275  TCCGGA              
AspHI        1000  GAGCTC               G[AT]GC[AT]^C
             1129  GAGCAC              
BanI          592  GGTACC               G^G[CT][GA]CC
             1091  GGCGCC              
BseAI         356  TCCGGA               T^CCGGA
             1275  TCCGGA              
BsiHKAI      1000  GAGCTC               G[AT]GC[AT]^C
             1129  GAGCAC              
BspEI         356  TCCGGA               T^CCGGA
             1275  TCCGGA              
BssSI         457  CACGAG               CACGAG(-5/-1)
              173  CTCGTG              
DdeI          290  CTCAG                C^T[ACGT]AG
             1250  CTGAG               
EarI          313  CTCTTC               CTCTTC(1/4)
              599  GAAGAG              
HgaI          764  GACGC                GACGC(5/10)
              970  GACGC               
Ksp632I       313  CTCTTC               CTCTTC(1/4)
              599  GAAGAG              
MroI          356  TCCGGA               T^CCGGA
             1275  TCCGGA              
MslI          487  CACGCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1236  CATGTACATG          
NspI          467  ACATGC               [GA]CATG^[CT]
             1235  ACATGT              
SfaNI         157  GATGC                GCATC(5/9)
              298  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsaWI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       TaqI        TseI        Tsp45I      
TthHB8I     
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