Restriction map for
pF1KB5169
(Linear) (Six-base) MAPSORT of: KIBB5169.seq Check: 2150 from: 1 to: 1311
KIBB5169.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 404 1311
Size: 404 907
Acc65I G'GTAC_C
Cuts at: 0 592 1311
Size: 592 719
AflIII A'CryG_T
Cuts at: 0 1235 1311
Size: 1235 76
AlwNI CAG_nnn'CTG
Cuts at: 0 12 1311
Size: 12 1299
ApaI G_GGCC'C
Cuts at: 0 897 1311
Size: 897 414
AvaI C'yCGr_G
Cuts at: 0 152 185 776 1003 1175 1311
Size: 152 33 591 227 172 136
Fragments arranged by size:
591 227 172 152 136 33
BanI G'GyrC_C
Cuts at: 0 592 1091 1311
Size: 592 499 220
Fragments arranged by size:
592 499 220
BanII G_rGCy'C
Cuts at: 0 897 956 1004 1311
Size: 897 59 48 307
Fragments arranged by size:
897 307 59 48
BbeI G_GCGC'C
Cuts at: 0 1095 1311
Size: 1095 216
BglI GCCn_nnn'nGGC
Cuts at: 0 570 1311
Size: 570 741
Bme1580I G_kGCm'C
Cuts at: 0 897 1311
Size: 897 414
BmgBI CAC'GTC
Cuts at: 0 531 1311
Size: 531 780
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 601 1311
Size: 601 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 579 813 977 1228 1311
Size: 94 485 234 164 251 83
Fragments arranged by size:
485 251 234 164 94 83
Bpu10I CC'TnA_GC
Cuts at: 0 290 1311
Size: 290 1021
BsaHI Gr'CG_yC
Cuts at: 0 1092 1311
Size: 1092 219
BsaWI w'CCGG_w
Cuts at: 0 356 860 1073 1275 1311
Size: 356 504 213 202 36
Fragments arranged by size:
504 356 213 202 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 1270 1300 1311
Size: 457 30 783 30 11
Fragments arranged by size:
783 457 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 155 205 593 617 829 934 1311
Size: 155 50 388 24 212 105 377
Fragments arranged by size:
388 377 212 155 105 50 24
BsiHKAI G_wGCw'C
Cuts at: 0 1004 1133 1311
Size: 1004 129 178
Fragments arranged by size:
1004 178 129
BsmI GAATG_Cn'
Cuts at: 0 627 1311
Size: 627 684
Bsp1286I G_dGCh'C
Cuts at: 0 897 956 1004 1133 1311
Size: 897 59 48 129 178
Fragments arranged by size:
897 178 129 59 48
BspEI T'CCGG_A
Cuts at: 0 356 1275 1311
Size: 356 919 36
Fragments arranged by size:
919 356 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 1311
Size: 71 1240
BsrBI CCG'CTC
Cuts at: 0 228 714 729 773 819 837 891 906
Size: 228 486 15 44 46 18 54 15
Cuts at: 906 1089 1146 1158 1311
Size: 183 57 12 153
Fragments arranged by size:
486 228 183 153 57 54 46 44
18 15 15 12
BsrDI GCAATG_nn'
Cuts at: 0 119 1311
Size: 119 1192
BsrFI r'CCGG_y
Cuts at: 0 782 1311
Size: 782 529
BsrGI T'GTAC_A
Cuts at: 0 1238 1311
Size: 1238 73
BssSI C'ACGA_G
Cuts at: 0 173 457 1311
Size: 173 284 854
Fragments arranged by size:
854 284 173
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 1311
Size: 27 1284
BtgI C'CryG_G
Cuts at: 0 429 1311
Size: 429 882
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 653 1311
Size: 653 658
BtsI GCAGTG_nn'
Cuts at: 0 1158 1311
Size: 1158 153
EaeI y'GGCC_r
Cuts at: 0 1191 1311
Size: 1191 120
EarI CTCTTCn'nnn_
Cuts at: 0 319 594 1311
Size: 319 275 717
Fragments arranged by size:
717 319 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 801 964 1080 1311
Size: 801 163 116 231
Fragments arranged by size:
801 231 163 116
EcoICRI GAG'CTC
Cuts at: 0 1002 1311
Size: 1002 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 579 813 977 1228 1311
Size: 94 485 234 164 251 83
Fragments arranged by size:
485 251 234 164 94 83
EcoO109I rG'GnC_Cy
Cuts at: 0 147 267 519 563 1311
Size: 147 120 252 44 748
Fragments arranged by size:
748 252 147 120 44
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 330 1311
Size: 330 981
HaeII r_GCGC'y
Cuts at: 0 795 828 1095 1311
Size: 795 33 267 216
Fragments arranged by size:
795 267 216 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 631 663 1311
Size: 457 32 142 32 648
Fragments arranged by size:
648 457 142 32 32
KasI G'GCGC_C
Cuts at: 0 1091 1311
Size: 1091 220
KpnI G_GTAC'C
Cuts at: 0 596 1311
Size: 596 715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1311
Size: 193 1118
MscI TGG'CCA
Cuts at: 0 1193 1311
Size: 1193 118
MslI CAynn'nnrTG
Cuts at: 0 491 1240 1311
Size: 491 749 71
Fragments arranged by size:
749 491 71
NarI GG'CG_CC
Cuts at: 0 1092 1311
Size: 1092 219
NruI TCG'CGA
Cuts at: 0 806 1311
Size: 806 505
NspI r_CATG'y
Cuts at: 0 471 1239 1311
Size: 471 768 72
Fragments arranged by size:
768 471 72
PciI A'CATG_T
Cuts at: 0 1235 1311
Size: 1235 76
PpuMI rG'GwC_Cy
Cuts at: 0 147 267 519 1311
Size: 147 120 252 792
Fragments arranged by size:
792 252 147 120
PspOMI G'GGCC_C
Cuts at: 0 893 1311
Size: 893 418
SacI G_AGCT'C
Cuts at: 0 1004 1311
Size: 1004 307
SanDI GG'GwC_CC
Cuts at: 0 267 1311
Size: 267 1044
SfcI C'TryA_G
Cuts at: 0 405 1311
Size: 405 906
SfoI GGC'GCC
Cuts at: 0 1093 1311
Size: 1093 218
SmaI CCC'GGG
Cuts at: 0 778 1311
Size: 778 533
SmlI C'TyrA_G
Cuts at: 0 152 1311
Size: 152 1159
StuI AGG'CCT
Cuts at: 0 584 1311
Size: 584 727
TatI w'GTAC_w
Cuts at: 0 1238 1311
Size: 1238 73
XhoI C'TCGA_G
Cuts at: 0 152 1311
Size: 152 1159
XmaI C'CCGG_G
Cuts at: 0 776 1311
Size: 776 535
Enzymes that do cut:
AccI Acc65I AflIII AlwNI ApaI AvaI BanI BanII
BbeI BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstXI BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I KasI KpnI MmeI MscI MslI NarI
NruI NspI PciI PpuMI PspOMI SacI SanDI SfcI
SfoI SmaI SmlI StuI TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BpuEI BsaI BsaAI
BsaBI BseYI BsgI BsiEI BsiWI BsmBI BspHI BssHII
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XmnI ZraI