(Linear) (Six-base) MAPSORT of: KIBB4809.seq Check: 416 from: 1 to: 2202 KIBB4809.orf With 182 enzymes: SgfI * March 3, 2008 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 293 2202 Size: 293 1909 AccI GT'mk_AC Cuts at: 0 1205 2202 Size: 1205 997 Acc65I G'GTAC_C Cuts at: 0 752 1963 2202 Size: 752 1211 239 Fragments arranged by size: 1211 752 239 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 700 1192 1696 1765 2202 Size: 439 261 492 504 69 437 Fragments arranged by size: 504 492 439 437 261 69 AflIII A'CryG_T Cuts at: 0 1208 2202 Size: 1208 994 AhdI GACnn_n'nnGTC Cuts at: 0 432 1266 2202 Size: 432 834 936 Fragments arranged by size: 936 834 432 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 946 978 2202 Size: 946 32 1224 Fragments arranged by size: 1224 946 32 AlwNI CAG_nnn'CTG Cuts at: 0 294 678 693 1170 1732 2202 Size: 294 384 15 477 562 470 Fragments arranged by size: 562 477 470 384 294 15 ApaI G_GGCC'C Cuts at: 0 1779 2202 Size: 1779 423 ApoI r'AATT_y Cuts at: 0 1275 2202 Size: 1275 927 AvaI C'yCGr_G Cuts at: 0 562 2202 Size: 562 1640 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 744 777 2202 Size: 744 33 1425 Fragments arranged by size: 1425 744 33 BanI G'GyrC_C Cuts at: 0 133 608 752 1963 2202 Size: 133 475 144 1211 239 Fragments arranged by size: 1211 475 239 144 133 BanII G_rGCy'C Cuts at: 0 946 1081 1779 2202 Size: 946 135 698 423 Fragments arranged by size: 946 698 423 135 BbeI G_GCGC'C Cuts at: 0 612 2202 Size: 612 1590 BbvCI CC'TCA_GC Cuts at: 0 1867 2202 Size: 1867 335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 447 481 553 587 2202 Size: 447 34 72 34 1615 Fragments arranged by size: 1615 447 72 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 566 1900 2202 Size: 566 1334 302 Fragments arranged by size: 1334 566 302 BlpI GC'TnA_GC Cuts at: 0 2014 2202 Size: 2014 188 Bme1580I G_kGCm'C Cuts at: 0 136 1039 1779 2202 Size: 136 903 740 423 Fragments arranged by size: 903 740 423 136 BmgBI CAC'GTC Cuts at: 0 1211 2202 Size: 1211 991 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1020 2202 Size: 1020 1182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 346 1794 1858 2202 Size: 316 30 1448 64 344 Fragments arranged by size: 1448 344 316 64 30 Bpu10I CC'TnA_GC Cuts at: 0 1867 2202 Size: 1867 335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 367 2020 2182 2202 Size: 160 207 1653 162 20 Fragments arranged by size: 1653 207 162 160 20 BsaAI yAC'GTr Cuts at: 0 1620 2202 Size: 1620 582 BsaBI GATnn'nnATC Cuts at: 0 1552 2202 Size: 1552 650 BsaHI Gr'CG_yC Cuts at: 0 53 392 609 2202 Size: 53 339 217 1593 Fragments arranged by size: 1593 339 217 53 BsaWI w'CCGG_w Cuts at: 0 1960 2202 Size: 1960 242 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 946 976 1286 1316 1792 1822 2202 Size: 946 30 310 30 476 30 380 Fragments arranged by size: 946 476 380 310 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 367 967 970 973 1130 1591 1927 2202 Size: 367 600 3 3 157 461 336 275 Fragments arranged by size: 600 461 367 336 275 157 3 3 BseYI C'CCAG_C Cuts at: 0 604 931 1163 1414 1481 2202 Size: 604 327 232 251 67 721 Fragments arranged by size: 721 604 327 251 232 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1600 2202 Size: 1600 602 BsiEI CG_ry'CG Cuts at: 0 141 1479 2202 Size: 141 1338 723 Fragments arranged by size: 1338 723 141 BsiHKAI G_wGCw'C Cuts at: 0 284 362 1571 2075 2117 2202 Size: 284 78 1209 504 42 85 Fragments arranged by size: 1209 504 284 85 78 42 BsmI GAATG_Cn' Cuts at: 0 407 2202 Size: 407 1795 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1483 2202 Size: 1483 719 Bsp1286I G_dGCh'C Cuts at: 0 136 284 362 946 1039 1081 1571 1779 Size: 136 148 78 584 93 42 490 208 Cuts at: 1779 2075 2117 2202 Size: 296 42 85 Fragments arranged by size: 584 490 296 208 148 136 93 85 78 42 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 293 1136 2202 Size: 293 843 1066 Fragments arranged by size: 1066 843 293 BsrBI CCG'CTC Cuts at: 0 1772 1808 2202 Size: 1772 36 394 Fragments arranged by size: 1772 394 36 BsrDI GCAATG_nn' Cuts at: 0 542 1055 2202 Size: 542 513 1147 Fragments arranged by size: 1147 542 513 BsrGI T'GTAC_A Cuts at: 0 263 2202 Size: 263 1939 BssSI C'ACGA_G Cuts at: 0 1786 2202 Size: 1786 416 BstAPI GCAn_nnn'nTGC Cuts at: 0 693 1732 2142 2172 2202 Size: 693 1039 410 30 30 Fragments arranged by size: 1039 693 410 30 30 BstEII G'GTnAC_C Cuts at: 0 327 786 2202 Size: 327 459 1416 Fragments arranged by size: 1416 459 327 BstXI CCAn_nnnn'nTGG Cuts at: 0 1305 2202 Size: 1305 897 BstYI r'GATC_y Cuts at: 0 225 449 2159 2202 Size: 225 224 1710 43 Fragments arranged by size: 1710 225 224 43 BstZ17I GTA'TAC Cuts at: 0 1206 2202 Size: 1206 996 BtgI C'CryG_G Cuts at: 0 711 1298 1382 2202 Size: 711 587 84 820 Fragments arranged by size: 820 711 587 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 450 999 1839 2152 2202 Size: 450 549 840 313 50 Fragments arranged by size: 840 549 450 313 50 DraIII CAC_nnn'GTG Cuts at: 0 444 2130 2202 Size: 444 1686 72 Fragments arranged by size: 1686 444 72 DrdI GACnn_nn'nnGTC Cuts at: 0 373 769 1158 2202 Size: 373 396 389 1044 Fragments arranged by size: 1044 396 389 373 EaeI y'GGCC_r Cuts at: 0 230 251 918 1307 1476 1595 1667 2202 Size: 230 21 667 389 169 119 72 535 Fragments arranged by size: 667 535 389 230 169 119 72 21 EagI C'GGCC_G Cuts at: 0 1476 2202 Size: 1476 726 EarI CTCTTCn'nnn_ Cuts at: 0 1933 2202 Size: 1933 269 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1307 2202 Size: 1307 895 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 346 439 700 1192 1696 1765 1794 Size: 316 30 93 261 492 504 69 29 Cuts at: 1794 1858 2202 Size: 64 344 Fragments arranged by size: 504 492 344 316 261 93 69 64 30 29 EcoO109I rG'GnC_Cy Cuts at: 0 246 1143 1260 1775 1917 2202 Size: 246 897 117 515 142 285 Fragments arranged by size: 897 515 285 246 142 117 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2061 2202 Size: 2061 141 FspI TGC'GCA Cuts at: 0 1852 2202 Size: 1852 350 FspAI rTGC'GCAy Cuts at: 0 1852 2202 Size: 1852 350 HaeII r_GCGC'y Cuts at: 0 102 612 2043 2202 Size: 102 510 1431 159 Fragments arranged by size: 1431 510 159 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 210 242 418 450 1106 1138 1252 1284 Size: 210 32 176 32 656 32 114 32 Cuts at: 1284 1286 1318 1792 1824 2151 2183 2202 Size: 2 32 474 32 327 32 19 Fragments arranged by size: 656 474 327 210 176 114 32 32 32 32 32 32 32 19 2 KasI G'GCGC_C Cuts at: 0 608 2202 Size: 608 1594 KpnI G_GTAC'C Cuts at: 0 756 1967 2202 Size: 756 1211 235 Fragments arranged by size: 1211 756 235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 368 404 1925 2202 Size: 368 36 1521 277 Fragments arranged by size: 1521 368 277 36 MscI TGG'CCA Cuts at: 0 232 1597 1669 2202 Size: 232 1365 72 533 Fragments arranged by size: 1365 533 232 72 MslI CAynn'nnrTG Cuts at: 0 547 898 1042 1762 1825 2202 Size: 547 351 144 720 63 377 Fragments arranged by size: 720 547 377 351 144 63 MspA1I CmG'CkG Cuts at: 0 90 291 521 1234 1442 1481 1836 2202 Size: 90 201 230 713 208 39 355 366 Fragments arranged by size: 713 366 355 230 208 201 90 39 NarI GG'CG_CC Cuts at: 0 609 2202 Size: 609 1593 NcoI C'CATG_G Cuts at: 0 1298 1382 2202 Size: 1298 84 820 Fragments arranged by size: 1298 820 84 NspI r_CATG'y Cuts at: 0 735 1606 2175 2202 Size: 735 871 569 27 Fragments arranged by size: 871 735 569 27 PasI CC'CwG_GG Cuts at: 0 775 1147 2202 Size: 775 372 1055 Fragments arranged by size: 1055 775 372 PfoI T'CCnGG_A Cuts at: 0 362 1270 2202 Size: 362 908 932 Fragments arranged by size: 932 908 362 PmlI CAC'GTG Cuts at: 0 1620 2202 Size: 1620 582 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 946 978 2202 Size: 946 32 1224 Fragments arranged by size: 1224 946 32 PpuMI rG'GwC_Cy Cuts at: 0 246 1143 1260 2202 Size: 246 897 117 942 Fragments arranged by size: 942 897 246 117 PspOMI G'GGCC_C Cuts at: 0 1775 2202 Size: 1775 427 PspXI vC'TCGA_Gb Cuts at: 0 562 2202 Size: 562 1640 PstI C_TGCA'G Cuts at: 0 95 290 689 1737 1886 2138 2202 Size: 95 195 399 1048 149 252 64 Fragments arranged by size: 1048 399 252 195 149 95 64 PvuII CAG'CTG Cuts at: 0 90 291 521 1234 1836 2202 Size: 90 201 230 713 602 366 Fragments arranged by size: 713 602 366 230 201 90 RsrII CG'GwC_CG Cuts at: 0 480 2202 Size: 480 1722 SanDI GG'GwC_CC Cuts at: 0 1143 1260 2202 Size: 1143 117 942 Fragments arranged by size: 1143 942 117 SapI GCTCTTCn'nnn_ Cuts at: 0 1933 2202 Size: 1933 269 ScaI AGT'ACT Cuts at: 0 202 2202 Size: 202 2000 SexAI A'CCwGG_T Cuts at: 0 440 2202 Size: 440 1762 SfcI C'TryA_G Cuts at: 0 30 91 286 685 1733 1882 2134 2202 Size: 30 61 195 399 1048 149 252 68 Fragments arranged by size: 1048 399 252 195 149 68 61 30 SfoI GGC'GCC Cuts at: 0 610 2202 Size: 610 1592 SmlI C'TyrA_G Cuts at: 0 175 382 562 2035 2197 2202 Size: 175 207 180 1473 162 5 Fragments arranged by size: 1473 207 180 175 162 5 SphI G_CATG'C Cuts at: 0 2175 2202 Size: 2175 27 StyI C'CwwG_G Cuts at: 0 308 488 1298 1382 1409 2202 Size: 308 180 810 84 27 793 Fragments arranged by size: 810 793 308 180 84 27 TatI w'GTAC_w Cuts at: 0 200 263 2202 Size: 200 63 1939 Fragments arranged by size: 1939 200 63 Tth111I GACn'n_nGTC Cuts at: 0 766 2202 Size: 766 1436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 621 2202 Size: 621 1581 XhoI C'TCGA_G Cuts at: 0 562 2202 Size: 562 1640 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AloI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtgZI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII RsrII SanDI SapI ScaI SexAI SfcI SfoI SmlI SphI StyI TatI Tth111I XcmI XhoI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AleI AlfI ApaLI AscI AseI AsiSI AvrII BamHI BbsI BciVI BclI BfrBI BglII BmrI BmtI BsaI BsiWI BspEI BspHI BsrFI BssHII BstBI Bsu36I BtsI ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FseI HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PshAI PsiI PsrI PvuI SacI SacII SalI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI XbaI XmaI XmnI ZraI