Restriction map for pF1KB4735
 (Linear) (Six-base) MAPSORT of: KIBB4735.seq  Check: 2630  from: 1  to: 1152



KIBB4735.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     849    1152 
   Size:        849     303
 
AflII C'TTAA_G

Cuts at:      0     184    1152 
   Size:        184     968
 
AflIII A'CryG_T

Cuts at:      0     909    1152 
   Size:        909     243
 
AleI CACnn'nnGTG

Cuts at:      0    1048    1152 
   Size:       1048     104
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     795    1152 
   Size:        795     357
 
ApoI r'AATT_y

Cuts at:      0     466     889    1001    1024    1152 
   Size:        466     423     112      23     128

  Fragments arranged by size: 

                 466     423     128     112      23
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     172     205    1152 
   Size:        172      33     947

  Fragments arranged by size: 

                 947     172      33
 
BamHI G'GATC_C

Cuts at:      0     591    1152 
   Size:        591     561
 
BanII G_rGCy'C

Cuts at:      0     286     859    1152 
   Size:        286     573     293

  Fragments arranged by size: 

                 573     293     286
 
BclI T'GATC_A

Cuts at:      0     948    1152 
   Size:        948     204
 
BglII A'GATC_T

Cuts at:      0     155    1152 
   Size:        155     997
 
Bme1580I G_kGCm'C

Cuts at:      0     876    1152 
   Size:        876     276
 
BmgBI CAC'GTC

Cuts at:      0     792    1152 
   Size:        792     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     873    1120    1152 
   Size:        300     573     247      32

  Fragments arranged by size: 

                 573     300     247      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117    1152 
   Size:        117    1035
 
BsaAI yAC'GTr

Cuts at:      0     912    1152 
   Size:        912     240
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      34      49      64    1152 
   Size:         19      15      15      15    1088

  Fragments arranged by size: 

                1088      19      15      15      15
 
BseYI C'CCAG_C

Cuts at:      0     564     991    1037    1152 
   Size:        564     427      46     115

  Fragments arranged by size: 

                 564     427     115      46
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     273     538    1152 
   Size:        273     265     614

  Fragments arranged by size: 

                 614     273     265
 
BsiHKAI G_wGCw'C

Cuts at:      0    1100    1152 
   Size:       1100      52
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     419    1152 
   Size:        419     733
 
Bsp1286I G_dGCh'C

Cuts at:      0     286     859     876    1100    1152 
   Size:        286     573      17     224      52

  Fragments arranged by size: 

                 573     286     224      52      17
 
BspHI T'CATG_A

Cuts at:      0     452    1152 
   Size:        452     700
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     847    1152 
   Size:        847     305
 
BsrGI T'GTAC_A

Cuts at:      0     298     494    1152 
   Size:        298     196     658

  Fragments arranged by size: 

                 658     298     196
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     561    1152 
   Size:        561     591
 
BstEII G'GTnAC_C

Cuts at:      0     261     339     834    1152 
   Size:        261      78     495     318

  Fragments arranged by size: 

                 495     318     261      78
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     321    1152 
   Size:        321     831
 
BstYI r'GATC_y

Cuts at:      0     155     591    1152 
   Size:        155     436     561

  Fragments arranged by size: 

                 561     436     155
 
BtsI GCAGTG_nn'

Cuts at:      0     929    1032    1152 
   Size:        929     103     120

  Fragments arranged by size: 

                 929     120     103
 
DraIII CAC_nnn'GTG

Cuts at:      0     296    1152 
   Size:        296     856
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     694    1152 
   Size:        694     458
 
EaeI y'GGCC_r

Cuts at:      0     311     323     983    1152 
   Size:        311      12     660     169

  Fragments arranged by size: 

                 660     311     169      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     849     873    1120    1152 
   Size:        300     549      24     247      32

  Fragments arranged by size: 

                 549     300     247      32      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     325     820     985    1152 
   Size:        325     495     165     167

  Fragments arranged by size: 

                 495     325     167     165
 
EcoO109I rG'GnC_Cy

Cuts at:      0     127     137     330     381     696    1152 
   Size:        127      10     193      51     315     456

  Fragments arranged by size: 

                 456     315     193     127      51      10
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      27     158    1152 
   Size:         27     131     994

  Fragments arranged by size: 

                 994     131      27
 
FspI TGC'GCA

Cuts at:      0     976    1152 
   Size:        976     176
 
HaeII r_GCGC'y

Cuts at:      0     403    1152 
   Size:        403     749
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66    1152 
   Size:         34      32    1086

  Fragments arranged by size: 

                1086      34      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     460    1152 
   Size:         67     393     692

  Fragments arranged by size: 

                 692     393      67
 
MscI TGG'CCA

Cuts at:      0     313    1152 
   Size:        313     839
 
MslI CAynn'nnrTG

Cuts at:      0    1048    1152 
   Size:       1048     104
 
MspA1I CmG'CkG

Cuts at:      0     869    1152 
   Size:        869     283
 
NspI r_CATG'y

Cuts at:      0     681    1152 
   Size:        681     471
 
PasI CC'CwG_GG

Cuts at:      0    1079    1152 
   Size:       1079      73
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     882    1152 
   Size:        882     270
 
PfoI T'CCnGG_A

Cuts at:      0     277     785     850    1152 
   Size:        277     508      65     302

  Fragments arranged by size: 

                 508     302     277      65
 
PmlI CAC'GTG

Cuts at:      0     912    1152 
   Size:        912     240
 
PpuMI rG'GwC_Cy

Cuts at:      0     127     330     381     696    1152 
   Size:        127     203      51     315     456

  Fragments arranged by size: 

                 456     315     203     127      51
 
PsiI TTA'TAA

Cuts at:      0      65    1152 
   Size:         65    1087
 
PvuII CAG'CTG

Cuts at:      0     869    1152 
   Size:        869     283
 
RsrII CG'GwC_CG

Cuts at:      0     430    1152 
   Size:        430     722
 
SanDI GG'GwC_CC

Cuts at:      0     127     381    1152 
   Size:        127     254     771

  Fragments arranged by size: 

                 771     254     127
 
SexAI A'CCwGG_T

Cuts at:      0     265    1152 
   Size:        265     887
 
SfcI C'TryA_G

Cuts at:      0     828    1152 
   Size:        828     324
 
SmlI C'TyrA_G

Cuts at:      0     132     184    1152 
   Size:        132      52     968

  Fragments arranged by size: 

                 968     132      52
 
SphI G_CATG'C

Cuts at:      0     681    1152 
   Size:        681     471
 
SspI AAT'ATT

Cuts at:      0     541    1152 
   Size:        541     611
 
StyI C'CwwG_G

Cuts at:      0     520     628    1152 
   Size:        520     108     524

  Fragments arranged by size: 

                 524     520     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     209     612    1152 
   Size:        209     403     540

  Fragments arranged by size: 

                 540     403     209
 
TatI w'GTAC_w

Cuts at:      0     298     494    1152 
   Size:        298     196     658

  Fragments arranged by size: 

                 658     298     196
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1081    1111    1152 
   Size:       1081      30      41

  Fragments arranged by size: 

                1081      41      30
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI     AlfI     ApoI     BaeI    BamHI
    BanII     BclI    BglII Bme1580I    BmgBI     BpmI    BpuEI    BsaAI
    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrGI   BstAPI   BstEII    BstXI    BstYI     BtsI   DraIII     DrdI
     EaeI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI     PsiI    PvuII    RsrII    SanDI    SexAI     SfcI
     SmlI     SphI     SspI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     EagI     EarI     EciI  EcoICRI
    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI     SacI    SacII
     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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