Restriction map for pF1KB4506
 (Linear) (Six-base) MAPSORT of: KIBB4506.seq  Check: 9762  from: 1  to: 1965



KIBB4506.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

Acc65I G'GTAC_C

Cuts at:      0     522    1965 
   Size:        522    1443
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1661    1965 
   Size:       1661     304
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1408    1965 
   Size:       1408     557
 
AlwNI CAG_nnn'CTG

Cuts at:      0     400     553    1965 
   Size:        400     153    1412

  Fragments arranged by size: 

                1412     400     153
 
ApaI G_GGCC'C

Cuts at:      0    1859    1965 
   Size:       1859     106
 
AvaI C'yCGr_G

Cuts at:      0    1502    1511    1671    1965 
   Size:       1502       9     160     294

  Fragments arranged by size: 

                1502     294     160       9
 
AvrII C'CTAG_G

Cuts at:      0     486    1965 
   Size:        486    1479
 
BamHI G'GATC_C

Cuts at:      0     733    1965 
   Size:        733    1232
 
BanI G'GyrC_C

Cuts at:      0     522    1965 
   Size:        522    1443
 
BanII G_rGCy'C

Cuts at:      0    1859    1965 
   Size:       1859     106
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1070    1256    1842    1965 
   Size:       1070     186     586     123

  Fragments arranged by size: 

                1070     586     186     123
 
BclI T'GATC_A

Cuts at:      0     609    1965 
   Size:        609    1356
 
BglI GCCn_nnn'nGGC

Cuts at:      0     250    1703    1965 
   Size:        250    1453     262

  Fragments arranged by size: 

                1453     262     250
 
BglII A'GATC_T

Cuts at:      0    1029    1965 
   Size:       1029     936
 
Bme1580I G_kGCm'C

Cuts at:      0    1859    1965 
   Size:       1859     106
 
BmgBI CAC'GTC

Cuts at:      0    1903    1965 
   Size:       1903      62
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     809    1184    1965 
   Size:        809     375     781

  Fragments arranged by size: 

                 809     781     375
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1744    1965 
   Size:       1744     221
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     866     998    1547    1574    1623    1965 
   Size:        866     132     549      27      49     342

  Fragments arranged by size: 

                 866     549     342     132      49      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1965 
   Size:         62    1903
 
BsaBI GATnn'nnATC

Cuts at:      0    1034    1192    1965 
   Size:       1034     158     773

  Fragments arranged by size: 

                1034     773     158
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1965 
   Size:          4    1961
 
BsaWI w'CCGG_w

Cuts at:      0     856     872    1965 
   Size:        856      16    1093

  Fragments arranged by size: 

                1093     856      16
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     543     573     867     897    1388    1418    1523    1553 
   Size:        543      30     294      30     491      30     105      30

Cuts at:   1553    1965 
   Size:        412

  Fragments arranged by size: 

                 543     491     412     294     105      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     968    1484    1493    1544    1910    1965 
   Size:        968     516       9      51     366      55

  Fragments arranged by size: 

                 968     516     366      55      51       9
 
BseYI C'CCAG_C

Cuts at:      0      68     481    1965 
   Size:         68     413    1484

  Fragments arranged by size: 

                1484     413      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     539    1965 
   Size:        386     153    1426

  Fragments arranged by size: 

                1426     386     153
 
BsiHKAI G_wGCw'C

Cuts at:      0    1937    1965 
   Size:       1937      28
 
Bsp1286I G_dGCh'C

Cuts at:      0    1859    1937    1965 
   Size:       1859      78      28

  Fragments arranged by size: 

                1859      78      28
 
BspEI T'CCGG_A

Cuts at:      0     856    1965 
   Size:        856    1109
 
BsrGI T'GTAC_A

Cuts at:      0    1089    1965 
   Size:       1089     876
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     400    1965 
   Size:        400    1565
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     106     277     526     822    1965 
   Size:        106     171     249     296    1143

  Fragments arranged by size: 

                1143     296     249     171     106
 
BstYI r'GATC_y

Cuts at:      0     733    1029    1053    1965 
   Size:        733     296      24     912

  Fragments arranged by size: 

                 912     733     296      24
 
BtgI C'CryG_G

Cuts at:      0       8    1013    1274    1407    1445    1464    1770    1794 
   Size:          8    1005     261     133      38      19     306      24

Cuts at:   1794    1965 
   Size:        171

  Fragments arranged by size: 

                1005     306     261     171     133      38      24      19
                   8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1244    1965 
   Size:       1244     721
 
BtsI GCAGTG_nn'

Cuts at:      0     170     302     398     551    1150    1259    1570    1965 
   Size:        170     132      96     153     599     109     311     395

  Fragments arranged by size: 

                 599     395     311     170     153     132     109      96
            
 
DraIII CAC_nnn'GTG

Cuts at:      0    1378    1408    1965 
   Size:       1378      30     557

  Fragments arranged by size: 

                1378     557      30
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1448    1965 
   Size:       1448     517
 
EaeI y'GGCC_r

Cuts at:      0    1767    1800    1965 
   Size:       1767      33     165

  Fragments arranged by size: 

                1767     165      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     903    1713    1887    1965 
   Size:        903     810     174      78

  Fragments arranged by size: 

                 903     810     174      78
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1655    1965 
   Size:       1655     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     866     998    1547    1574    1623    1661    1965 
   Size:        866     132     549      27      49      38     304

  Fragments arranged by size: 

                 866     549     304     132      49      38      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1204    1493    1965 
   Size:       1204     289     472

  Fragments arranged by size: 

                1204     472     289
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1432    1441    1498    1629    1855    1856    1909    1965 
   Size:       1432       9      57     131     226       1      53      56

  Fragments arranged by size: 

                1432     226     131      57      56      53       9       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1342    1965 
   Size:       1342     623
 
HaeII r_GCGC'y

Cuts at:      0     977    1965 
   Size:        977     988
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1051    1083    1523    1555    1965 
   Size:       1051      32     440      32     410

  Fragments arranged by size: 

                1051     440     410      32      32
 
HincII GTy'rAC

Cuts at:      0     448     682    1965 
   Size:        448     234    1283

  Fragments arranged by size: 

                1283     448     234
 
KpnI G_GTAC'C

Cuts at:      0     526    1965 
   Size:        526    1439
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     825    1965 
   Size:        262     563    1140

  Fragments arranged by size: 

                1140     563     262
 
NcoI C'CATG_G

Cuts at:      0    1013    1445    1965 
   Size:       1013     432     520

  Fragments arranged by size: 

                1013     520     432
 
NdeI CA'TA_TG

Cuts at:      0     105     351    1965 
   Size:        105     246    1614

  Fragments arranged by size: 

                1614     246     105
 
NspI r_CATG'y

Cuts at:      0    1395    1710    1965 
   Size:       1395     315     255

  Fragments arranged by size: 

                1395     315     255
 
PasI CC'CwG_GG

Cuts at:      0    1849    1965 
   Size:       1849     116
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     379    1408    1965 
   Size:        379    1029     557

  Fragments arranged by size: 

                1029     557     379
 
PfoI T'CCnGG_A

Cuts at:      0    1416    1965 
   Size:       1416     549
 
PpuMI rG'GwC_Cy

Cuts at:      0    1432    1441    1498    1909    1965 
   Size:       1432       9      57     411      56

  Fragments arranged by size: 

                1432     411      57      56       9
 
PspOMI G'GGCC_C

Cuts at:      0    1855    1965 
   Size:       1855     110
 
PstI C_TGCA'G

Cuts at:      0      39    1965 
   Size:         39    1926
 
SanDI GG'GwC_CC

Cuts at:      0    1441    1965 
   Size:       1441     524
 
SfcI C'TryA_G

Cuts at:      0      24      35      51     308     507     738    1049    1965 
   Size:         24      11      16     257     199     231     311     916

  Fragments arranged by size: 

                 916     311     257     231     199      24      16      11
            
 
SmaI CCC'GGG

Cuts at:      0    1504    1673    1965 
   Size:       1504     169     292

  Fragments arranged by size: 

                1504     292     169
 
SmlI C'TyrA_G

Cuts at:      0      77    1965 
   Size:         77    1888
 
SpeI A'CTAG_T

Cuts at:      0     442     601     712    1965 
   Size:        442     159     111    1253

  Fragments arranged by size: 

                1253     442     159     111
 
SphI G_CATG'C

Cuts at:      0    1395    1710    1965 
   Size:       1395     315     255

  Fragments arranged by size: 

                1395     315     255
 
StuI AGG'CCT

Cuts at:      0     328    1965 
   Size:        328    1637
 
StyI C'CwwG_G

Cuts at:      0     486    1013    1265    1445    1965 
   Size:        486     527     252     180     520

  Fragments arranged by size: 

                 527     520     486     252     180
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     112     337     814    1272    1829    1965 
   Size:        112     225     477     458     557     136

  Fragments arranged by size: 

                 557     477     458     225     136     112
 
TatI w'GTAC_w

Cuts at:      0    1089    1965 
   Size:       1089     876
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     543     575     867     899    1388    1420    1965 
   Size:        543      32     292      32     489      32     545

  Fragments arranged by size: 

                 545     543     489     292      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1116    1965 
   Size:       1116     849
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1272    1452    1549    1965 
   Size:       1272     180      97     416

  Fragments arranged by size: 

                1272     416     180      97
 
XmaI C'CCGG_G

Cuts at:      0    1502    1671    1965 
   Size:       1502     169     294

  Fragments arranged by size: 

                1502     294     169
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI    AlwNI     ApaI     AvaI    AvrII    BamHI
     BanI    BanII     BbsI     BclI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BsrGI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII     KpnI     MmeI     NcoI     NdeI     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    SanDI     SfcI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BlpI     BmtI
   Bpu10I     BsaI    BsaAI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SrfI     SspI     SwaI  Tth111I     XhoI
     XmnI     ZraI
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