Restriction map for pF1KB4489
 (Linear) (Six-base) MAPSORT of: KIBB4489.seq  Check: 1597  from: 1  to: 1401



KIBB4489.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     228    1401 
   Size:        228    1173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1376    1401 
   Size:        340    1036      25

  Fragments arranged by size: 

                1036     340      25
 
AflIII A'CryG_T

Cuts at:      0     584     753    1401 
   Size:        584     169     648

  Fragments arranged by size: 

                 648     584     169
 
AleI CACnn'nnGTG

Cuts at:      0    1338    1401 
   Size:       1338      63
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     274    1084    1401 
   Size:        274     810     317

  Fragments arranged by size: 

                 810     317     274
 
AlwNI CAG_nnn'CTG

Cuts at:      0     932    1136    1179    1344    1401 
   Size:        932     204      43     165      57

  Fragments arranged by size: 

                 932     204     165      57      43
 
ApaLI G'TGCA_C

Cuts at:      0     603    1006    1401 
   Size:        603     403     395

  Fragments arranged by size: 

                 603     403     395
 
AvaI C'yCGr_G

Cuts at:      0     183    1401 
   Size:        183    1218
 
BanII G_rGCy'C

Cuts at:      0     174     809    1372    1401 
   Size:        174     635     563      29

  Fragments arranged by size: 

                 635     563     174      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1143    1401 
   Size:       1143     258
 
BlpI GC'TnA_GC

Cuts at:      0     460    1172    1401 
   Size:        460     712     229

  Fragments arranged by size: 

                 712     460     229
 
Bme1580I G_kGCm'C

Cuts at:      0     607    1010    1401 
   Size:        607     403     391

  Fragments arranged by size: 

                 607     403     391
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     588    1179    1401 
   Size:        588     591     222

  Fragments arranged by size: 

                 591     588     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     349     392    1401 
   Size:        184     165      43    1009

  Fragments arranged by size: 

                1009     184     165      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1129    1401 
   Size:       1129     272
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     706    1401 
   Size:        706     695
 
BsaWI w'CCGG_w

Cuts at:      0    1241    1401 
   Size:       1241     160
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     574     604     607     637    1401 
   Size:        574      30       3      30     764

  Fragments arranged by size: 

                 764     574      30      30       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     817    1401 
   Size:        817     584
 
BseYI C'CCAG_C

Cuts at:      0     193     455     577     809    1107    1129    1401 
   Size:        193     262     122     232     298      22     272

  Fragments arranged by size: 

                 298     272     262     232     193     122      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     545    1090    1401 
   Size:        478      67     545     311

  Fragments arranged by size: 

                 545     478     311      67
 
BsiEI CG_ry'CG

Cuts at:      0     827    1401 
   Size:        827     574
 
BsiHKAI G_wGCw'C

Cuts at:      0     428     559     607     823     890    1010    1401 
   Size:        428     131      48     216      67     120     391

  Fragments arranged by size: 

                 428     391     216     131     120      67      48
 
Bsp1286I G_dGCh'C

Cuts at:      0     174     428     559     607     809     823     890    1010 
   Size:        174     254     131      48     202      14      67     120

Cuts at:   1010    1372    1401 
   Size:        362      29

  Fragments arranged by size: 

                 362     254     202     174     131     120      67      48
                  29      14
 
BspEI T'CCGG_A

Cuts at:      0    1241    1401 
   Size:       1241     160
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     228    1401 
   Size:        228    1173
 
BsrBI CCG'CTC

Cuts at:      0     218    1401 
   Size:        218    1183
 
BssSI C'ACGA_G

Cuts at:      0     313     427    1401 
   Size:        313     114     974

  Fragments arranged by size: 

                 974     313     114
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1304    1401 
   Size:       1304      97
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1340    1401 
   Size:       1340      61
 
BstYI r'GATC_y

Cuts at:      0     297    1124    1401 
   Size:        297     827     277

  Fragments arranged by size: 

                 827     297     277
 
BtgI C'CryG_G

Cuts at:      0     152    1197    1401 
   Size:        152    1045     204

  Fragments arranged by size: 

                1045     204     152
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1264    1401 
   Size:       1264     137
 
BtsI GCAGTG_nn'

Cuts at:      0      56     116    1333    1345    1401 
   Size:         56      60    1217      12      56

  Fragments arranged by size: 

                1217      60      56      56      12
 
ClaI AT'CG_AT

Cuts at:      0     766    1401 
   Size:        766     635
 
EaeI y'GGCC_r

Cuts at:      0     292     470     608     723    1401 
   Size:        292     178     138     115     678

  Fragments arranged by size: 

                 678     292     178     138     115
 
EarI CTCTTCn'nnn_

Cuts at:      0      34      49      80    1171    1401 
   Size:         34      15      31    1091     230

  Fragments arranged by size: 

                1091     230      34      31      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     340     349     392    1376    1401 
   Size:        184     156       9      43     984      25

  Fragments arranged by size: 

                 984     184     156      43      25       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255    1401 
   Size:        255    1146
 
HaeII r_GCGC'y

Cuts at:      0     291     488    1401 
   Size:        291     197     913

  Fragments arranged by size: 

                 913     291     197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     261     289     293     321     642     674    1356    1388 
   Size:        261      28       4      28     321      32     682      32

Cuts at:   1388    1401 
   Size:         13

  Fragments arranged by size: 

                 682     321     261      32      32      28      28      13
                   4
 
HincII GTy'rAC

Cuts at:      0     672    1401 
   Size:        672     729
 
MluI A'CGCG_T

Cuts at:      0     584    1401 
   Size:        584     817
 
MscI TGG'CCA

Cuts at:      0     472     725    1401 
   Size:        472     253     676

  Fragments arranged by size: 

                 676     472     253
 
MslI CAynn'nnrTG

Cuts at:      0    1066    1325    1338    1401 
   Size:       1066     259      13      63

  Fragments arranged by size: 

                1066     259      63      13
 
MspA1I CmG'CkG

Cuts at:      0     179     327     745    1133    1401 
   Size:        179     148     418     388     268

  Fragments arranged by size: 

                 418     388     268     179     148
 
NcoI C'CATG_G

Cuts at:      0     152    1401 
   Size:        152    1249
 
NspI r_CATG'y

Cuts at:      0     757    1401 
   Size:        757     644
 
PciI A'CATG_T

Cuts at:      0     753    1401 
   Size:        753     648
 
PstI C_TGCA'G

Cuts at:      0     239     305    1141    1340    1401 
   Size:        239      66     836     199      61

  Fragments arranged by size: 

                 836     239     199      66      61
 
PvuII CAG'CTG

Cuts at:      0     179     745    1401 
   Size:        179     566     656

  Fragments arranged by size: 

                 656     566     179
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1171    1401 
   Size:       1171     230
 
ScaI AGT'ACT

Cuts at:      0     413    1401 
   Size:        413     988
 
SfcI C'TryA_G

Cuts at:      0     235     301     731    1137    1336    1401 
   Size:        235      66     430     406     199      65

  Fragments arranged by size: 

                 430     406     235     199      66      65
 
SmaI CCC'GGG

Cuts at:      0     185    1401 
   Size:        185    1216
 
SmlI C'TyrA_G

Cuts at:      0    1144    1401 
   Size:       1144     257
 
StyI C'CwwG_G

Cuts at:      0     152    1401 
   Size:        152    1249
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     591     813    1121    1401 
   Size:        591     222     308     280

  Fragments arranged by size: 

                 591     308     280     222
 
TatI w'GTAC_w

Cuts at:      0     411    1013    1234    1401 
   Size:        411     602     221     167

  Fragments arranged by size: 

                 602     411     221     167
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     574     606     989    1021    1401 
   Size:        574      32     383      32     380

  Fragments arranged by size: 

                 574     383     380      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     159    1401 
   Size:        159    1242
 
XmaI C'CCGG_G

Cuts at:      0     183    1401 
   Size:        183    1218
 
XmnI GAAnn'nnTTC

Cuts at:      0     636     928    1401 
   Size:        636     292     473

  Fragments arranged by size: 

                 636     473     292
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI     AvaI
    BanII     BbsI     BlpI Bme1580I     BmrI     BpmI    BpuEI     BsaI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrBI    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI     EaeI     EarI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     MluI     MscI     MslI   MspA1I     NcoI     NspI
     PciI     PstI    PvuII     SapI     ScaI     SfcI     SmaI     SmlI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     ZraI
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