Restriction map for pF1KB4411
 (Circular) (Six-base) MAPSORT of: pF1KB4411.seq  Check: 8131  from: 1  to: 3671



pF1KB4411  3671 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:    709     709 
   Size:       3671
 
Acc65I G'GTAC_C

Cuts at:    687     687 
   Size:       3671
 
AclI AA'CG_TT

Cuts at:   3511    3511 
   Size:       3671
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1496    1928    2675    2943    1496 
   Size:        432     747     268    2224

  Fragments arranged by size: 

                2224     747     432     268
 
AflIII A'CryG_T

Cuts at:   1023    2127    3104    1023 
   Size:       1104     977    1590

  Fragments arranged by size: 

                1590    1104     977
 
AgeI A'CCGG_T

Cuts at:   2059    2059 
   Size:       3671
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    144     144 
   Size:       3671
 
AlwNI CAG_nnn'CTG

Cuts at:    262     546     976    2543     262 
   Size:        284     430    1567    1390

  Fragments arranged by size: 

                1567    1390     430     284
 
ApaI G_GGCC'C

Cuts at:    660     660 
   Size:       3671
 
ApaLI G'TGCA_C

Cuts at:   2441    2441 
   Size:       3671
 
ApoI r'AATT_y

Cuts at:    675    3024    3280     675 
   Size:       2349     256    1066

  Fragments arranged by size: 

                2349    1066     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3671
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3671
 
AvaI C'yCGr_G

Cuts at:    521     691     521 
   Size:        170    3501
 
BamHI G'GATC_C

Cuts at:    696     696 
   Size:       3671
 
BanI G'GyrC_C

Cuts at:    687    1101    1349    1384     687 
   Size:        414     248      35    2974

  Fragments arranged by size: 

                2974     414     248      35
 
BanII G_rGCy'C

Cuts at:    198     660     685    1715     198 
   Size:        462      25    1030    2154

  Fragments arranged by size: 

                2154    1030     462      25
 
BbeI G_GCGC'C

Cuts at:   1105    1353    1105 
   Size:        248    3423
 
BbsI GAAGACnn'nnnn_

Cuts at:    253     253 
   Size:       3671
 
BbvCI CC'TCA_GC

Cuts at:    515     515 
   Size:       3671
 
BciVI GTATCCnnnnn_n'

Cuts at:   1569    2330    3671    1569 
   Size:        761    1341    1569

  Fragments arranged by size: 

                1569    1341     761
 
BclI T'GATC_A

Cuts at:   1191    1191 
   Size:       3671
 
BfrBI ATG'CAT

Cuts at:    131     131 
   Size:       3671
 
BglII A'GATC_T

Cuts at:    176    1186    3223     176 
   Size:       1010    2037     624

  Fragments arranged by size: 

                2037    1010     624
 
BlpI GC'TnA_GC

Cuts at:    783     783 
   Size:       3671
 
Bme1580I G_kGCm'C

Cuts at:    660    1296    1389    2445     660 
   Size:        636      93    1056    1886

  Fragments arranged by size: 

                1886    1056     636      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    883     964    1299    3443     883 
   Size:         81     335    2144    1111

  Fragments arranged by size: 

                2144    1111     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2038    2038 
   Size:       3671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    425     439     425 
   Size:         14    3657
 
Bpu10I CC'TnA_GC

Cuts at:    515     515 
   Size:       3671
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    847    1726    2218    2516     847 
   Size:        879     492     298    2002

  Fragments arranged by size: 

                2002     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    137    3375     137 
   Size:       3238     433
 
BsaAI yAC'GTr

Cuts at:   1024    1024 
   Size:       3671
 
BsaBI GATnn'nnATC

Cuts at:      6    1185    3222       6 
   Size:       1179    2037     455

  Fragments arranged by size: 

                2037    1179     455
 
BsaHI Gr'CG_yC

Cuts at:   1102    1350    2052    3548    1102 
   Size:        248     702    1496    1225

  Fragments arranged by size: 

                1496    1225     702     248
 
BsaWI w'CCGG_w

Cuts at:    860    1381    2059    2333    2480    2891     860 
   Size:        521     678     274     147     411    1640

  Fragments arranged by size: 

                1640     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    263     293     263 
   Size:         30    3641
 
BseYI C'CCAG_C

Cuts at:    147     534    1096    1854    2431    2927     147 
   Size:        387     562     758     577     496     891

  Fragments arranged by size: 

                 891     758     577     562     496     387
 
BsiEI CG_ry'CG

Cuts at:    104    1213    1259    2467     104 
   Size:       1109      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1109      46
 
BsiHKAI G_wGCw'C

Cuts at:    685    1463    1653    2445     685 
   Size:        778     190     792    1911

  Fragments arranged by size: 

                1911     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    198     660     685    1296    1389    1463    1653    1715    2445 
   Size:        462      25     611      93      74     190      62     730

Cuts at:   2445     198 
   Size:       1424

  Fragments arranged by size: 

                1424     730     611     462     190      93      74      62
                  25
 
BspEI T'CCGG_A

Cuts at:   2891    2891 
   Size:       3671
 
BspHI T'CATG_A

Cuts at:   2847    2847 
   Size:       3671
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    721     935    1237    1618     721 
   Size:        214     302     381    2774

  Fragments arranged by size: 

                2774     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1963    2017    3501    3666      50 
   Size:       1913      54    1484     165      55

  Fragments arranged by size: 

                1913    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1583    1583 
   Size:       3671
 
BsrFI r'CCGG_y

Cuts at:    490    1669    1850    2059     490 
   Size:       1179     181     209    2102

  Fragments arranged by size: 

                2102    1179     209     181
 
BssSI C'ACGA_G

Cuts at:   1942    2300    1942 
   Size:        358    3313
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1048    1048 
   Size:       3671
 
BstBI TT'CG_AA

Cuts at:    587    2032     587 
   Size:       1445    2226
 
BstYI r'GATC_y

Cuts at:      1     176     696    1163    1186    1521    1767    2779    2865 
   Size:        175     520     467      23     335     246    1012      86

Cuts at:   2865    2877    3223       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     520     467     449     346     335     246     175
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3671
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1621    1802    1965    1621 
   Size:        181     163    3327

  Fragments arranged by size: 

                3327     181     163
 
BtsI GCAGTG_nn'

Cuts at:    158     192     509     600     914     158 
   Size:         34     317      91     314    2915

  Fragments arranged by size: 

                2915     317     314      91      34
 
DraI TTT'AAA

Cuts at:    670     670 
   Size:       3671
 
DrdI GACnn_nn'nnGTC

Cuts at:   1377    2235    1377 
   Size:        858    2813
 
EaeI y'GGCC_r

Cuts at:    488    1256    1430    1821    1848    3193     488 
   Size:        768     174     391      27    1345     966

  Fragments arranged by size: 

                1345     966     768     391     174      27
 
EagI C'GGCC_G

Cuts at:   1256    1256 
   Size:       3671
 
EarI CTCTTCn'nnn_

Cuts at:    292    1694    1904     292 
   Size:       1402     210    2059

  Fragments arranged by size: 

                2059    1402     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1879    2189    2335    3481    1879 
   Size:        310     146    1146    2069

  Fragments arranged by size: 

                2069    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    683     683 
   Size:       3671
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    425     439    1496    1928    2675    2943     425 
   Size:         14    1057     432     747     268    1153

  Fragments arranged by size: 

                1153    1057     747     432     268      14
 
EcoO109I rG'GnC_Cy

Cuts at:    543     657     810     543 
   Size:        114     153    3404

  Fragments arranged by size: 

                3404     153     114
 
EcoRI G'AATT_C

Cuts at:    675     675 
   Size:       3671
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1690    1690 
   Size:       3671
 
FspI TGC'GCA

Cuts at:   1452    1452 
   Size:       3671
 
HaeII r_GCGC'y

Cuts at:    535    1105    1353    2375    3354     535 
   Size:        570     248    1022     979     852

  Fragments arranged by size: 

                1022     979     852     570     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1513    1543    1545    1575    1513 
   Size:         30       2      30    3609

  Fragments arranged by size: 

                3609      30      30       2
 
HincII GTy'rAC

Cuts at:    710     710 
   Size:       3671
 
KasI G'GCGC_C

Cuts at:   1101    1349    1101 
   Size:        248    3423
 
KpnI G_GTAC'C

Cuts at:    691     691 
   Size:       3671
 
MluI A'CGCG_T

Cuts at:   3104    3104 
   Size:       3671
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2342    2526    2342 
   Size:        184    3487
 
MscI TGG'CCA

Cuts at:   1432    1432 
   Size:       3671
 
MslI CAynn'nnrTG

Cuts at:   1660    1787    1660 
   Size:        127    3544
 
MspA1I CmG'CkG

Cuts at:    514     627     782    1096    1456    2469    2714     514 
   Size:        113     155     314     360    1013     245    1471

  Fragments arranged by size: 

                1471    1013     360     314     245     155     113
 
NaeI GCC'GGC

Cuts at:    492    1852     492 
   Size:       1360    2311
 
NarI GG'CG_CC

Cuts at:   1102    1350    1102 
   Size:        248    3423
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3671
 
NgoMIV G'CCGG_C

Cuts at:    490    1850     490 
   Size:       1360    2311
 
NsiI A_TGCA'T

Cuts at:    133     133 
   Size:       3671
 
NspI r_CATG'y

Cuts at:    724    1224    2131     724 
   Size:        500     907    2264

  Fragments arranged by size: 

                2264     907     500
 
PciI A'CATG_T

Cuts at:   2127    2127 
   Size:       3671
 
PflMI CCAn_nnn'nTGG

Cuts at:    546     546 
   Size:       3671
 
PmeI GTTT'AAAC

Cuts at:    670     670 
   Size:       3671
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2810    2842    2810 
   Size:         32    3639
 
PspOMI G'GGCC_C

Cuts at:    656     656 
   Size:       3671
 
PstI C_TGCA'G

Cuts at:    409     718    1403     409 
   Size:        309     685    2677

  Fragments arranged by size: 

                2677     685     309
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3671
 
PvuII CAG'CTG

Cuts at:    514     627    1096    1456     514 
   Size:        113     469     360    2729

  Fragments arranged by size: 

                2729     469     360     113
 
RsrII CG'GwC_CG

Cuts at:   1866    1866 
   Size:       3671
 
SacI G_AGCT'C

Cuts at:    685     685 
   Size:       3671
 
SalI G'TCGA_C

Cuts at:    708     708 
   Size:       3671
 
SapI GCTCTTCn'nnn_

Cuts at:   1694    1904    1694 
   Size:        210    3461
 
SbfI CC_TGCA'GG

Cuts at:    718     718 
   Size:       3671
 
ScaI AGT'ACT

Cuts at:   3188    3188 
   Size:       3671
 
SfcI C'TryA_G

Cuts at:     33     405     714    1399    2392    2583      33 
   Size:        372     309     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     372     309     191
 
SfoI GGC'GCC

Cuts at:   1103    1351    1103 
   Size:        248    3423
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3671
 
SmaI CCC'GGG

Cuts at:    523     693     523 
   Size:        170    3501
 
SmlI C'TyrA_G

Cuts at:    826    1741    2233    2495     826 
   Size:        915     492     262    2002

  Fragments arranged by size: 

                2002     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3671
 
SphI G_CATG'C

Cuts at:    724    1224     724 
   Size:        500    3171
 
SrfI GCCC'GGGC

Cuts at:    523     523 
   Size:       3671
 
StyI C'CwwG_G

Cuts at:    107     805    1159     107 
   Size:        698     354    2619

  Fragments arranged by size: 

                2619     698     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    419    1254    1387    1846    2055     419 
   Size:        835     133     459     209    2035

  Fragments arranged by size: 

                2035     835     459     209     133
 
TatI w'GTAC_w

Cuts at:   3186    3186 
   Size:       3671
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    164     196     263     295     325     357    3188    3220     164 
   Size:         32      67      32      30      32    2831      32     615

  Fragments arranged by size: 

                2831     615      67      32      32      32      32      30
            
 
Tth111I GACn'n_nGTC

Cuts at:   1468    1468 
   Size:       3671
 
XbaI T'CTAG_A

Cuts at:    702     702 
   Size:       3671
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     132    1166     114 
   Size:         18    1034    2619

  Fragments arranged by size: 

                2619    1034      18
 
XmaI C'CCGG_G

Cuts at:    521     691     521 
   Size:        170    3501
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI    PflMI     PmeI     PpiI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SrfI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI     BcgI     BglI    BmgBI     BmtI    BseRI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI
     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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