Restriction map for pF1KB4062
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmI        BsoBI       
BspEI       BspHI       BspLU11I    BsrDI       BssHII      
Bst1107I    BstBI       BstXI       BstZ17I     CelII       
CpoI        DraI        DraIII      DrdI        DsaI        
EarI        Eco47III    EcoNI       EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HindIII     KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcyI          340  GACGCC               G[GA]^CG[CT]C
AfaI           90  GTAC                 GT^AC
AhdI          240  GACCCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          470  CCTCAGG              CC^T[ACGT]AGG
AspEI         240  GACCCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI          44  GGATCC               G^GATCC
BanII         493  GGGCTC               G[GA]GC[CT]^C
BcnI          425  CCCGG                CC^[GC]GG
BglI          213  GCCGCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          493  GGGCTC               G[AGT]GC[ACT]^C
BsaHI         340  GACGCC               G[GA]^CG[CT]C
BsaI          247  GGTCTC               GGTCTC(1/5)
BsaJI         243  CCAAGG               C^C[ACGT][ACGT]GG
BsmBI         148  GAGACG               CGTCTC(1/5)
Bsp1286I      493  GGGCTC               G[AGT]GC[ACT]^C
Bsp1407I       89  TGTACA               T^GTACA
BspDI         286  ATCGAT               AT^CGAT
BspMI         374  ACCTGC               ACCTGC(4/8)
BsrBI         214  CCGCTC               CCGCTC(-3/-3)
BsrFI          70  GCCGGC               [GA]^CCGG[CT]
BsrGI          89  TGTACA               T^GTACA
BssSI         397  CACGAG               CACGAG(-5/-1)
BstAPI        369  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        114  GGTGACC              G^GT[ACGT]ACC
BstF5I        186  GGATG                GGATG(2/0)
BstPI         114  GGTGACC              G^GT[ACGT]ACC
Bsu36I        470  CCTCAGG              CC^T[ACGT]AGG
CfoI          269  GCGC                 GCG^C
Cfr10I         70  GCCGGC               [GA]^CCGG[CT]
ClaI          286  ATCGAT               AT^CGAT
DdeI          471  CTCAG                C^T[ACGT]AG
Eam1105I      240  GACCCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        470  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       114  GGTGACC              G^GT[ACGT]ACC
EcoT14I       243  CCAAGG               C^C[AT][AT]GG
FokI          186  GGATG                GGATG(9/13)
HhaI          269  GCGC                 GCG^C
Hin1I         340  GACGCC               G[GA]^CG[CT]C
HinP1I        269  GCGC                 G^CGC
HincII        504  GTTAAC               GT[CT]^[GA]AC
HindII        504  GTTAAC               GT[CT]^[GA]AC
HpaI          504  GTTAAC               GTT^AAC
MseI          505  TTAA                 T^TAA
NaeI           70  GCCGGC               GCC^GGC
NciI          425  CCCGG                CC^[GC]GG
NgoMIV         70  GCCGGC               G^CCGGC
PleI          482  GACTC                GAGTC(4/5)
RsaI           90  GTAC                 GT^AC
SspBI          89  TGTACA               T^GTACA
StyI          243  CCAAGG               C^C[AT][AT]GG
TaqI          287  TCGA                 T^CGA
TfiI          429  GATTC                G^A[AT]TC
Tru9I         505  TTAA                 T^TAA
TthHB8I       287  TCGA                 T^CGA
XcmI          122  CCATTCCCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          66  CGCG                 CG^CG
              277  CGCG                
AluI          101  AGCT                 AG^CT
              305  AGCT                
AvaII         239  GGACC                G^G[AT]CC
              372  GGACC               
BbvI          453  GCAGC                GCAGC(8/12)
              306  GCTGC               
BsgI          307  CTGCAC               GTGCAG(16/14)
              451  GTGCAG              
BsmAI         148  GAGAC                GTCTC(1/5)
              248  GTCTC               
BstUI          66  CGCG                 CG^CG
              277  CGCG                
Cac8I          70  GCCGGC               GC[ACGT]^[ACGT]GC
              295  GCTCGC              
DraII         238  GGGACCC              [GA]G^G[ACGT]CC[CT]
              371  AGGACCT             
EagI           68  CGGCCG               C^GGCCG
              211  CGGCCG              
EclXI          68  CGGCCG               C^GGCCG
              211  CGGCCG              
Eco52I         68  CGGCCG               C^GGCCG
              211  CGGCCG              
EcoO109I      238  GGGACCC              [GA]G^G[ACGT]CC[CT]
              371  AGGACCT             
HgaI           64  GACGC                GACGC(5/10)
              340  GACGC               
HinfI         429  GATTC                G^A[ACGT]TC
              482  GACTC               
HphI          114  GGTGA                GGTGA(8/7)
              153  GGTGA               
MaeII         143  ACGT                 A^CGT
              179  ACGT                
MaeIII        115  GTGAC                ^GT[ACGT]AC
              154  GTGAC               
MvnI           66  CGCG                 CG^CG
              277  CGCG                
PpuMI         238  GGGACCC              [GA]G^G[AT]CC[CT]
              371  AGGACCT             
SfaNI         382  GATGC                GCATC(5/9)
              284  GCATC               
TseI          306  GCTGC                G^C[AT]GC
              453  GCAGC               
Tsp45I        115  GTGAC                ^GT[GC]AC
              154  GTGAC               
TspRI         129  CACTG                CA[GC]TG[ACGT][ACGT]^
              234  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AlwI        AlwNI       BsiEI       BsiYI       
BslI        BsmFI       BsrI        BssKI       BstNI       
BstYI       Cfr13I      DpnII       EaeI        EcoRII      
Fnu4HI      HaeIII      HapII       HpaII       ItaI        
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       XhoII       
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