Restriction map for
pF1KB4046
(Circular) (Six-base) MAPSORT of: pF1KB4046.seq Check: 9874 from: 1 to: 4448
pF1KB4046 4448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1203 1203
Size: 4448
AccI GT'mk_AC
Cuts at: 1193 1270 1486 1193
Size: 77 216 4155
Fragments arranged by size:
4155 216 77
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 814 2273 2705 3058 3720 133
Size: 681 1459 432 353 662 861
Fragments arranged by size:
1459 861 681 662 432 353
AfeI AGC'GCT
Cuts at: 231 231
Size: 4448
AflII C'TTAA_G
Cuts at: 1327 1327
Size: 4448
AflIII A'CryG_T
Cuts at: 1800 3600 3881 1800
Size: 1800 281 2367
Fragments arranged by size:
2367 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1329 1329
Size: 4448
AleI CACnn'nnGTG
Cuts at: 1413 1413
Size: 4448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 637 637
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 208 1753 3191 208
Size: 1545 1438 1465
Fragments arranged by size:
1545 1465 1438
ApaLI G'TGCA_C
Cuts at: 1123 3286 1123
Size: 2163 2285
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 260 1468 260
Size: 1208 3240
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 363 621 1401 1464 1878 2126 2161 363
Size: 258 780 63 414 248 35 2650
Fragments arranged by size:
2650 780 414 258 248 63 35
BanII G_rGCy'C
Cuts at: 509 932 950 1462 2492 509
Size: 423 18 512 1030 2465
Fragments arranged by size:
2465 1030 512 423 18
BbeI G_GCGC'C
Cuts at: 1405 1882 2130 1405
Size: 477 248 3723
Fragments arranged by size:
3723 477 248
BbsI GAAGACnn'nnnn_
Cuts at: 687 687
Size: 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4414
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3402 4448 2346
Size: 1056 1046 2346
Fragments arranged by size:
2346 1056 1046
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglI GCCn_nnn'nGGC
Cuts at: 954 954
Size: 4448
BglII A'GATC_T
Cuts at: 1028 1963 4000 1028
Size: 935 2037 1476
Fragments arranged by size:
2037 1476 935
BlpI GC'TnA_GC
Cuts at: 503 938 1560 503
Size: 435 622 3391
Fragments arranged by size:
3391 622 435
Bme1580I G_kGCm'C
Cuts at: 368 542 1127 2073 2166 3290 368
Size: 174 585 946 93 1124 1526
Fragments arranged by size:
1526 1124 946 585 174 93
BmgBI CAC'GTC
Cuts at: 1415 1415
Size: 4448
BmrI ACTGGGnnnn_n'
Cuts at: 1660 1741 2076 4220 1660
Size: 81 335 2144 1888
Fragments arranged by size:
2144 1888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1198 2815 1198
Size: 1617 2831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 763 964 322
Size: 441 201 3806
Fragments arranged by size:
3806 441 201
Bpu10I CC'TnA_GC
Cuts at: 607 872 607
Size: 265 4183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1017 1078 1624 2503 3217 3515 1017
Size: 61 546 879 714 298 1950
Fragments arranged by size:
1950 879 714 546 298 61
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 1801 1801
Size: 4448
BsaBI GATnn'nnATC
Cuts at: 6 427 1962 3999 6
Size: 421 1535 2037 455
Fragments arranged by size:
2037 1535 455 421
BsaHI Gr'CG_yC
Cuts at: 287 860 1402 1879 2127 2829 4325 287
Size: 573 542 477 248 702 1496 410
Fragments arranged by size:
1496 702 573 542 477 410 248
BsaWI w'CCGG_w
Cuts at: 1238 1637 2158 2836 3247 3394 3668 1238
Size: 399 521 678 411 147 274 2018
Fragments arranged by size:
2018 678 521 411 399 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 937 967 937
Size: 30 4418
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 559 172
Size: 387 4061
BseYI C'CCAG_C
Cuts at: 201 386 677 684 1133 1205 1248 1873 2631
Size: 185 291 7 449 72 43 625 758
Cuts at: 2631 3296 3704 201
Size: 665 408 945
Fragments arranged by size:
945 758 665 625 449 408 291 185
72 43 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1285
Size: 4448
BsiEI CG_ry'CG
Cuts at: 104 722 991 1990 2036 3266 104
Size: 618 269 999 46 1230 1286
Fragments arranged by size:
1286 1230 999 618 269 46
BsiHKAI G_wGCw'C
Cuts at: 509 932 1127 1203 1462 2240 2430 3290 509
Size: 423 195 76 259 778 190 860 1667
Fragments arranged by size:
1667 860 778 423 259 195 190 76
BsmI GAATG_Cn'
Cuts at: 174 174
Size: 4448
BsmBI CGTCTCn'nnnn_
Cuts at: 541 825 541
Size: 284 4164
Bsp1286I G_dGCh'C
Cuts at: 368 509 542 932 950 1127 1203 1462 2073
Size: 141 33 390 18 177 76 259 611
Cuts at: 2073 2166 2240 2430 2492 3290 368
Size: 93 74 190 62 798 1526
Fragments arranged by size:
1526 798 611 390 259 190 177 141
93 76 74 62 33 18
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1160 1203 1498 1712 2014 2395 1160
Size: 43 295 214 302 381 3213
Fragments arranged by size:
3213 381 302 295 214 43
BsrBI CCG'CTC
Cuts at: 50 306 2740 2794 4278 4443 50
Size: 256 2434 54 1484 165 55
Fragments arranged by size:
2434 1484 256 165 55 54
BsrDI GCAATG_nn'
Cuts at: 614 2360 614
Size: 1746 2702
BsrFI r'CCGG_y
Cuts at: 414 2446 2627 414
Size: 2032 181 2235
Fragments arranged by size:
2235 2032 181
BssHII G'CGCG_C
Cuts at: 664 664
Size: 4448
BssSI C'ACGA_G
Cuts at: 541 2719 3427 541
Size: 2178 708 1562
Fragments arranged by size:
2178 1562 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1122 1825 1122
Size: 703 3745
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 1308 1415 1308
Size: 107 4341
BstYI r'GATC_y
Cuts at: 1 1028 1473 1940 1963 2298 2544 2850 2862
Size: 1027 445 467 23 335 246 306 12
Cuts at: 2862 2948 4000 1
Size: 86 1052 449
Fragments arranged by size:
1052 1027 467 449 445 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1271 1271
Size: 4448
BtgI C'CryG_G
Cuts at: 371 716 722 1068 371
Size: 345 6 346 3751
Fragments arranged by size:
3751 346 345 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 423 546 2398 2579 2742 423
Size: 123 1852 181 163 2129
Fragments arranged by size:
2129 1852 181 163 123
BtsI GCAGTG_nn'
Cuts at: 1691 1691
Size: 4448
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3498 2154
Size: 1344 3104
EaeI y'GGCC_r
Cuts at: 719 923 955 988 1050 2033 2207 2598 2625
Size: 204 32 33 62 983 174 391 27
Cuts at: 2625 3970 719
Size: 1345 1197
Fragments arranged by size:
1345 1197 983 391 204 174 62 33
32 27
EagI C'GGCC_G
Cuts at: 719 988 2033 719
Size: 269 1045 3134
Fragments arranged by size:
3134 1045 269
EarI CTCTTCn'nnn_
Cuts at: 190 514 2471 2681 190
Size: 324 1957 210 1957
Fragments arranged by size:
1957 1957 324 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 3398 3544 4258 2656
Size: 742 146 714 2846
Fragments arranged by size:
2846 742 714 146
EcoICRI GAG'CTC
Cuts at: 507 930 1460 507
Size: 423 530 3495
Fragments arranged by size:
3495 530 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 322 763 814 964 2273 2705 3058 3720
Size: 189 441 51 150 1309 432 353 662
Cuts at: 3720 133
Size: 861
Fragments arranged by size:
1309 861 662 441 432 353 189 150
51
EcoO109I rG'GnC_Cy
Cuts at: 1587 1587
Size: 4448
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 149 233 468 685 1405 1882 2130 3360 4131
Size: 84 235 217 720 477 248 1230 771
Cuts at: 4131 149
Size: 466
Fragments arranged by size:
1230 771 720 477 466 248 235 217
84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 1315 1347 2290 2320 2322 2352 328
Size: 32 955 32 943 30 2 30 2424
Fragments arranged by size:
2424 955 943 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 1401 1878 2126 1401
Size: 477 248 3723
Fragments arranged by size:
3723 477 248
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3207 3391 3207
Size: 184 4264
MscI TGG'CCA
Cuts at: 957 2209 957
Size: 1252 3196
MslI CAynn'nnrTG
Cuts at: 619 1413 2437 2564 619
Size: 794 1024 127 2503
Fragments arranged by size:
2503 1024 794 127
MspA1I CmG'CkG
Cuts at: 354 373 677 718 789 999 1137 1158 1209
Size: 19 304 41 71 210 138 21 51
Cuts at: 1209 1559 1873 2233 3017 3262 354
Size: 350 314 360 784 245 1540
Fragments arranged by size:
1540 784 360 350 314 304 245 210
138 71 51 41 21 19
NaeI GCC'GGC
Cuts at: 416 2629 416
Size: 2213 2235
NarI GG'CG_CC
Cuts at: 1402 1879 2127 1402
Size: 477 248 3723
Fragments arranged by size:
3723 477 248
NgoMIV G'CCGG_C
Cuts at: 414 2627 414
Size: 2213 2235
NspI r_CATG'y
Cuts at: 1501 2001 3604 1501
Size: 500 1603 2345
Fragments arranged by size:
2345 1603 500
PciI A'CATG_T
Cuts at: 3600 3600
Size: 4448
PflMI CCAn_nnn'nTGG
Cuts at: 208 208
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 4416
PstI C_TGCA'G
Cuts at: 129 326 1157 1214 1495 2180 129
Size: 197 831 57 281 685 2397
Fragments arranged by size:
2397 831 685 281 197 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1137 1209 1873 2233 1137
Size: 72 664 360 3352
Fragments arranged by size:
3352 664 360 72
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 509 932 1462 509
Size: 423 530 3495
Fragments arranged by size:
3495 530 423
SacII CC_GC'GG
Cuts at: 374 719 374
Size: 345 4103
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 514 2471 2681 514
Size: 1957 210 2281
Fragments arranged by size:
2281 1957 210
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 769 3965 769
Size: 3196 1252
SfcI C'TryA_G
Cuts at: 33 125 322 1153 1210 1491 2176 3144 3335
Size: 92 197 831 57 281 685 968 191
Cuts at: 3335 33
Size: 1146
Fragments arranged by size:
1146 968 831 685 281 197 191 92
57
SfoI GGC'GCC
Cuts at: 1403 1880 2128 1403
Size: 477 248 3723
Fragments arranged by size:
3723 477 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SgrAI Cr'CCGG_yG
Cuts at: 414 414
Size: 4448
SmaI CCC'GGG
Cuts at: 262 1470 262
Size: 1208 3240
SmlI C'TyrA_G
Cuts at: 1032 1093 1327 1603 2518 3232 3494 1032
Size: 61 234 276 915 714 262 1986
Fragments arranged by size:
1986 915 714 276 262 234 61
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StuI AGG'CCT
Cuts at: 870 964 1165 870
Size: 94 201 4153
Fragments arranged by size:
4153 201 94
StyI C'CwwG_G
Cuts at: 1301 1582 1936 1301
Size: 281 354 3813
Fragments arranged by size:
3813 354 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1147 1187 1300 2031 2164 2623 2832 1147
Size: 40 113 731 133 459 209 2763
Fragments arranged by size:
2763 731 459 209 133 113 40
TatI w'GTAC_w
Cuts at: 767 3963 767
Size: 3196 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 937 969 3965 3997 937
Size: 32 2996 32 1388
Fragments arranged by size:
2996 1388 32 32
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 965 1943 965
Size: 978 3470
XmaI C'CCGG_G
Cuts at: 260 1468 260
Size: 1208 3240
XmnI GAAnn'nnTTC
Cuts at: 269 269
Size: 4448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AgeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BspEI BsrGI BstEII Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XhoI ZraI