Restriction map for pF1KB4046
 (Linear) (Six-base) MAPSORT of: KIBB4046.seq  Check: 9774  from: 1  to: 1335



KIBB4046.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1095    1335 
   Size:       1095     240
 
AccI GT'mk_AC

Cuts at:      0    1085    1162    1335 
   Size:       1085      77     173

  Fragments arranged by size: 

                1085     173      77
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     706    1335 
   Size:         25     681     629

  Fragments arranged by size: 

                 681     629      25
 
AfeI AGC'GCT

Cuts at:      0     123    1335 
   Size:        123    1212
 
AflII C'TTAA_G

Cuts at:      0    1219    1335 
   Size:       1219     116
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1221    1335 
   Size:       1221     114
 
AleI CACnn'nnGTG

Cuts at:      0    1305    1335 
   Size:       1305      30
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     529    1335 
   Size:        529     806
 
AlwNI CAG_nnn'CTG

Cuts at:      0     100    1335 
   Size:        100    1235
 
ApaLI G'TGCA_C

Cuts at:      0    1015    1335 
   Size:       1015     320
 
AvaI C'yCGr_G

Cuts at:      0     152    1335 
   Size:        152    1183
 
BanI G'GyrC_C

Cuts at:      0     255     513    1293    1335 
   Size:        255     258     780      42

  Fragments arranged by size: 

                 780     258     255      42
 
BanII G_rGCy'C

Cuts at:      0     401     824     842    1335 
   Size:        401     423      18     493

  Fragments arranged by size: 

                 493     423     401      18
 
BbeI G_GCGC'C

Cuts at:      0    1297    1335 
   Size:       1297      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     579    1335 
   Size:        579     756
 
BglI GCCn_nnn'nGGC

Cuts at:      0     846    1335 
   Size:        846     489
 
BglII A'GATC_T

Cuts at:      0     920    1335 
   Size:        920     415
 
BlpI GC'TnA_GC

Cuts at:      0     395     830    1335 
   Size:        395     435     505

  Fragments arranged by size: 

                 505     435     395
 
Bme1580I G_kGCm'C

Cuts at:      0     260     434    1019    1335 
   Size:        260     174     585     316

  Fragments arranged by size: 

                 585     316     260     174
 
BmgBI CAC'GTC

Cuts at:      0    1307    1335 
   Size:       1307      28
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1090    1335 
   Size:       1090     245
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     655     856    1335 
   Size:        214     441     201     479

  Fragments arranged by size: 

                 479     441     214     201
 
Bpu10I CC'TnA_GC

Cuts at:      0     499     764    1335 
   Size:        499     265     571

  Fragments arranged by size: 

                 571     499     265
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     909     970    1335 
   Size:        909      61     365

  Fragments arranged by size: 

                 909     365      61
 
BsaBI GATnn'nnATC

Cuts at:      0     319    1335 
   Size:        319    1016
 
BsaHI Gr'CG_yC

Cuts at:      0     179     752    1294    1335 
   Size:        179     573     542      41

  Fragments arranged by size: 

                 573     542     179      41
 
BsaWI w'CCGG_w

Cuts at:      0    1130    1335 
   Size:       1130     205
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     829     859    1335 
   Size:        829      30     476

  Fragments arranged by size: 

                 829     476      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     451    1335 
   Size:         64     387     884

  Fragments arranged by size: 

                 884     387      64
 
BseYI C'CCAG_C

Cuts at:      0      93     278     569     576    1025    1097    1140    1335 
   Size:         93     185     291       7     449      72      43     195

  Fragments arranged by size: 

                 449     291     195     185      93      72      43       7
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    1335 
   Size:       1177     158
 
BsiEI CG_ry'CG

Cuts at:      0     614     883    1335 
   Size:        614     269     452

  Fragments arranged by size: 

                 614     452     269
 
BsiHKAI G_wGCw'C

Cuts at:      0     401     824    1019    1095    1335 
   Size:        401     423     195      76     240

  Fragments arranged by size: 

                 423     401     240     195      76
 
BsmI GAATG_Cn'

Cuts at:      0      66    1335 
   Size:         66    1269
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     433     717    1335 
   Size:        433     284     618

  Fragments arranged by size: 

                 618     433     284
 
Bsp1286I G_dGCh'C

Cuts at:      0     260     401     434     824     842    1019    1095    1335 
   Size:        260     141      33     390      18     177      76     240

  Fragments arranged by size: 

                 390     260     240     177     141      76      33      18
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1052    1095    1335 
   Size:       1052      43     240

  Fragments arranged by size: 

                1052     240      43
 
BsrBI CCG'CTC

Cuts at:      0     198    1335 
   Size:        198    1137
 
BsrDI GCAATG_nn'

Cuts at:      0     506    1335 
   Size:        506     829
 
BsrFI r'CCGG_y

Cuts at:      0     306    1335 
   Size:        306    1029
 
BssHII G'CGCG_C

Cuts at:      0     556    1335 
   Size:        556     779
 
BssSI C'ACGA_G

Cuts at:      0     433    1335 
   Size:        433     902
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1014    1335 
   Size:       1014     321
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1200    1307    1335 
   Size:       1200     107      28

  Fragments arranged by size: 

                1200     107      28
 
BstYI r'GATC_y

Cuts at:      0     920    1335 
   Size:        920     415
 
BstZ17I GTA'TAC

Cuts at:      0    1163    1335 
   Size:       1163     172
 
BtgI C'CryG_G

Cuts at:      0     263     608     614     960    1335 
   Size:        263     345       6     346     375

  Fragments arranged by size: 

                 375     346     345     263       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     315     438    1335 
   Size:        315     123     897

  Fragments arranged by size: 

                 897     315     123
 
EaeI y'GGCC_r

Cuts at:      0     611     815     847     880     942    1335 
   Size:        611     204      32      33      62     393

  Fragments arranged by size: 

                 611     393     204      62      33      32
 
EagI C'GGCC_G

Cuts at:      0     611     880    1335 
   Size:        611     269     455

  Fragments arranged by size: 

                 611     455     269
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     406    1335 
   Size:         82     324     929

  Fragments arranged by size: 

                 929     324      82
 
EcoICRI GAG'CTC

Cuts at:      0     399     822    1335 
   Size:        399     423     513

  Fragments arranged by size: 

                 513     423     399
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     214     655     706     856    1335 
   Size:         25     189     441      51     150     479

  Fragments arranged by size: 

                 479     441     189     150      51      25
 
HaeII r_GCGC'y

Cuts at:      0      41     125     360     577    1297    1335 
   Size:         41      84     235     217     720      38

  Fragments arranged by size: 

                 720     235     217      84      41      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252    1207    1239    1335 
   Size:        220      32     955      32      96

  Fragments arranged by size: 

                 955     220      96      32      32
 
KasI G'GCGC_C

Cuts at:      0    1293    1335 
   Size:       1293      42
 
MscI TGG'CCA

Cuts at:      0     849    1335 
   Size:        849     486
 
MslI CAynn'nnrTG

Cuts at:      0     511    1305    1335 
   Size:        511     794      30

  Fragments arranged by size: 

                 794     511      30
 
MspA1I CmG'CkG

Cuts at:      0     246     265     569     610     681     891    1029    1050 
   Size:        246      19     304      41      71     210     138      21

Cuts at:   1050    1101    1335 
   Size:         51     234

  Fragments arranged by size: 

                 304     246     234     210     138      71      51      41
                  21      19
 
NaeI GCC'GGC

Cuts at:      0     308    1335 
   Size:        308    1027
 
NarI GG'CG_CC

Cuts at:      0    1294    1335 
   Size:       1294      41
 
NgoMIV G'CCGG_C

Cuts at:      0     306    1335 
   Size:        306    1029
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     100    1335 
   Size:        100    1235
 
PstI C_TGCA'G

Cuts at:      0      21     218    1049    1106    1335 
   Size:         21     197     831      57     229

  Fragments arranged by size: 

                 831     229     197      57      21
 
PvuII CAG'CTG

Cuts at:      0    1029    1101    1335 
   Size:       1029      72     234

  Fragments arranged by size: 

                1029     234      72
 
SacI G_AGCT'C

Cuts at:      0     401     824    1335 
   Size:        401     423     511

  Fragments arranged by size: 

                 511     423     401
 
SacII CC_GC'GG

Cuts at:      0     266     611    1335 
   Size:        266     345     724

  Fragments arranged by size: 

                 724     345     266
 
SapI GCTCTTCn'nnn_

Cuts at:      0     406    1335 
   Size:        406     929
 
ScaI AGT'ACT

Cuts at:      0     661    1335 
   Size:        661     674
 
SfcI C'TryA_G

Cuts at:      0      17     214    1045    1102    1335 
   Size:         17     197     831      57     233

  Fragments arranged by size: 

                 831     233     197      57      17
 
SfoI GGC'GCC

Cuts at:      0    1295    1335 
   Size:       1295      40
 
SgrAI Cr'CCGG_yG

Cuts at:      0     306    1335 
   Size:        306    1029
 
SmaI CCC'GGG

Cuts at:      0     154    1335 
   Size:        154    1181
 
SmlI C'TyrA_G

Cuts at:      0     924     985    1219    1335 
   Size:        924      61     234     116

  Fragments arranged by size: 

                 924     234     116      61
 
StuI AGG'CCT

Cuts at:      0     762     856    1057    1335 
   Size:        762      94     201     278

  Fragments arranged by size: 

                 762     278     201      94
 
StyI C'CwwG_G

Cuts at:      0    1193    1335 
   Size:       1193     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1039    1079    1192    1335 
   Size:       1039      40     113     143

  Fragments arranged by size: 

                1039     143     113      40
 
TatI w'GTAC_w

Cuts at:      0     659    1335 
   Size:        659     676
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     829     861    1335 
   Size:        829      32     474

  Fragments arranged by size: 

                 829     474      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     857    1335 
   Size:        857     478
 
XmaI C'CCGG_G

Cuts at:      0     152    1335 
   Size:        152    1183
 
XmnI GAAnn'nnTTC

Cuts at:      0     161    1335 
   Size:        161    1174
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII     AhdI     AleI     AlfI
    AlwNI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BglI
    BglII     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV    PflMI     PstI
    PvuII     SacI    SacII     SapI     ScaI     SfcI     SfoI    SgrAI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI   AflIII     AgeI     AloI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BmrI     BmtI     BsaI    BsaAI    BsiWI
    BspEI    BspHI    BsrGI    BstBI   BstEII   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SalI    SanDI     SbfI    SexAI     SfiI     SgfI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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