Restriction map for pF1KB3987
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AvaI        
AvrII       BalI        BamHI       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BseAI       BsgI        
BsiHKAI     BsiWI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
DraI        DraIII      DrdI        Eam1105I    Eco81I      
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
HpaI        KpnI        KspI        MaeIII      MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
SacI        SacII       SalI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tsp45I      Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         976  GACGTC               GACGT^C
AlwNI          24  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       983  AGCGCT               AGC^GCT
ApaI           56  GGGCCC               GGGCC^C
AspI          772  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         230  TGCGCA               TGC^GCA
BanI          164  GGCGCC               G^G[CT][GA]CC
BbeI          164  GGCGCC               GGCGC^C
BbsI          915  GAAGAC               GAAGAC(2/6)
BbvCI          21  CCTCAGC              CCTCAGC(-5/-2)
BglI           49  GCCGGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         915  GAAGAC               GAAGAC(2/6)
BsaBI         228  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         560  ACCGGA               [AT]^CCGG[AT]
BseRI         756  CTCCTC               GAGGAG(10/8)
BsmI          347  GCATTC               GAATGC(1/-1)
BssSI         332  CACGAG               CACGAG(-5/-1)
BstXI         800  CCAACCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI          158  CGGTCCG              CG^G[AT]CCG
DsaI          240  CCACGG               C^C[GA][CT]GG
Eco47III      983  AGCGCT               AGC^GCT
EcoNI         780  CCTCATGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         598  GATATC               GAT^ATC
FspI          230  TGCGCA               TGC^GCA
HincII        841  GTCAAC               GT[CT]^[GA]AC
HindII        841  GTCAAC               GT[CT]^[GA]AC
HindIII       340  AAGCTT               A^AGCTT
KasI          164  GGCGCC               G^GCGCC
MamI          228  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          164  GGCGCC               GG^CGCC
PflFI         772  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         776  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         834  GACCATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         158  CGGTCCG              CG^G[AT]CCG
SapI          852  GCTCTTC              GCTCTTC(1/4)
SbfI         1110  CCTGCAGG             CCTGCA^GG
ScaI          539  AGTACT               AGT^ACT
SfiI           48  GGCCGGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          164  GGCGCC               GGC^GCC
Sse8387I     1110  CCTGCAGG             CCTGCA^GG
TfiI          478  GATTC                G^A[AT]TC
Tth111I       772  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI          164  GGCGCC               G[GA]^CG[CT]C
              976  GACGTC              
BanII          56  GGGCCC               G[GA]GC[CT]^C
              309  GGGCTC              
BfaI          587  CTAG                 C^TAG
              611  CTAG                
BmyI           56  GGGCCC               G[AGT]GC[ACT]^C
              309  GGGCTC              
BsaHI         164  GGCGCC               G[GA]^CG[CT]C
              976  GACGTC              
BsaI          832  GAGACC               GGTCTC(1/5)
              753  GGTCTC              
BsiEI          41  CGGCCG               CG[GA][CT]^CG
              243  CGGCCG              
BsmBI         864  CGTCTC               CGTCTC(1/5)
             1014  GAGACG              
BsmFI         451  GTCCC                GGGAC(10/14)
              696  GTCCC               
Bsp1286I       56  GGGCCC               G[AGT]GC[ACT]^C
              309  GGGCTC              
BsrBI         148  GAGCGG               CCGCTC(-3/-3)
              201  GAGCGG              
BsrI          717  CCAGT                ACTGG(1/-1)
              996  CCAGT               
BstYI         176  AGATCC               [GA]^GATC[CT]
              659  AGATCC              
DdeI           22  CTCAG                C^T[ACGT]AG
              460  CTGAG               
DraII          55  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              776  AGGTCCT             
EagI           41  CGGCCG               C^GGCCG
              243  CGGCCG              
EclXI          41  CGGCCG               C^GGCCG
              243  CGGCCG              
Eco52I         41  CGGCCG               C^GGCCG
              243  CGGCCG              
EcoO109I       55  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              776  AGGTCCT             
EcoT14I       352  CCTTGG               C^C[AT][AT]GG
              623  CCAAGG              
HgaI           91  GACGC                GACGC(5/10)
              130  GACGC               
Hin1I         164  GGCGCC               G[GA]^CG[CT]C
              976  GACGTC              
MaeI          587  CTAG                 C^TAG
              611  CTAG                
MflI          176  AGATCC               [GA]^GATC[CT]
              659  AGATCC              
MseI          653  TTAA                 T^TAA
              668  TTAA                
PleI          529  GAGTC                GAGTC(4/5)
              814  GAGTC               
PstI         1111  CTGCAG               CTGCA^G
             1138  CTGCAG              
SexAI         773  ACCAGGT              A^CC[AT]GGT
              989  ACCTGGT             
SfcI         1111  CTGCAG               C^T[GA][CT]AG
             1138  CTGCAG              
StyI          352  CCTTGG               C^C[AT][AT]GG
              623  CCAAGG              
Tru9I         653  TTAA                 T^TAA
              668  TTAA                
Tsp509I       577  AATT                 ^AATT
              630  AATT                
TspRI         718  CAGTG                CA[GC]TG[ACGT][ACGT]^
              997  CAGTG               
XhoII         176  AGATCC               [GA]^GATC[CT]
              659  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
Asp700I     AvaII       BbvI        BcnI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsrFI       
BssHII      BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DpnII       
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TseI        
TthHB8I     XcmI        XmnI        
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