Restriction map for pF1KB3987
 (Linear) (Six-base) MAPSORT of: KIBB3987.seq  Check: 8521  from: 1  to: 1143



KIBB3987.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AatII G_ACGT'C

Cuts at:      0     980    1143 
   Size:        980     163
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     933    1143 
   Size:        933     210
 
AfeI AGC'GCT

Cuts at:      0     985    1143 
   Size:        985     158
 
AlwNI CAG_nnn'CTG

Cuts at:      0      29    1143 
   Size:         29    1114
 
ApaI G_GGCC'C

Cuts at:      0      60    1143 
   Size:         60    1083
 
BanI G'GyrC_C

Cuts at:      0     164    1143 
   Size:        164     979
 
BanII G_rGCy'C

Cuts at:      0      60     313    1143 
   Size:         60     253     830

  Fragments arranged by size: 

                 830     253      60
 
BbeI G_GCGC'C

Cuts at:      0     168    1143 
   Size:        168     975
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     922    1143 
   Size:        922     221
 
BbvCI CC'TCA_GC

Cuts at:      0      22    1143 
   Size:         22    1121
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55    1143 
   Size:         55    1088
 
Bme1580I G_kGCm'C

Cuts at:      0      60    1143 
   Size:         60    1083
 
BmgBI CAC'GTC

Cuts at:      0     737    1143 
   Size:        737     406
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     492     744     991    1143 
   Size:        292     200     252     247     152

  Fragments arranged by size: 

                 292     252     247     200     152
 
Bpu10I CC'TnA_GC

Cuts at:      0      22     460    1143 
   Size:         22     438     683

  Fragments arranged by size: 

                 683     438      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     289     297    1143 
   Size:        205      84       8     846

  Fragments arranged by size: 

                 846     205      84       8
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     759     826    1143 
   Size:        759      67     317

  Fragments arranged by size: 

                 759     317      67
 
BsaBI GATnn'nnATC

Cuts at:      0     232    1143 
   Size:        232     911
 
BsaHI Gr'CG_yC

Cuts at:      0     165     977    1143 
   Size:        165     812     166

  Fragments arranged by size: 

                 812     166     165
 
BsaWI w'CCGG_w

Cuts at:      0     560    1143 
   Size:        560     583
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     747    1143 
   Size:        747     396
 
BseYI C'CCAG_C

Cuts at:      0     186     522     698    1143 
   Size:        186     336     176     445

  Fragments arranged by size: 

                 445     336     186     176
 
BsiEI CG_ry'CG

Cuts at:      0      44     246    1143 
   Size:         44     202     897

  Fragments arranged by size: 

                 897     202      44
 
BsmI GAATG_Cn'

Cuts at:      0     347    1143 
   Size:        347     796
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     870    1008    1143 
   Size:        870     138     135

  Fragments arranged by size: 

                 870     138     135
 
Bsp1286I G_dGCh'C

Cuts at:      0      60     313    1143 
   Size:         60     253     830

  Fragments arranged by size: 

                 830     253      60
 
BsrBI CCG'CTC

Cuts at:      0     150     203    1143 
   Size:        150      53     940

  Fragments arranged by size: 

                 940     150      53
 
BsrFI r'CCGG_y

Cuts at:      0      39      49     429     803    1143 
   Size:         39      10     380     374     340

  Fragments arranged by size: 

                 380     374     340      39      10
 
BssHII G'CGCG_C

Cuts at:      0      83      85     153    1143 
   Size:         83       2      68     990

  Fragments arranged by size: 

                 990      83      68       2
 
BssSI C'ACGA_G

Cuts at:      0     332    1143 
   Size:        332     811
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     807    1143 
   Size:        807     336
 
BstYI r'GATC_y

Cuts at:      0     176     659    1143 
   Size:        176     483     484

  Fragments arranged by size: 

                 484     483     176
 
BtgI C'CryG_G

Cuts at:      0     240    1143 
   Size:        240     903
 
EaeI y'GGCC_r

Cuts at:      0      41     243     431     567     687    1143 
   Size:         41     202     188     136     120     456

  Fragments arranged by size: 

                 456     202     188     136     120      41
 
EagI C'GGCC_G

Cuts at:      0      41     243    1143 
   Size:         41     202     900

  Fragments arranged by size: 

                 900     202      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     859     883    1006    1042    1143 
   Size:        859      24     123      36     101

  Fragments arranged by size: 

                 859     123     101      36      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     492     744     933     991    1143 
   Size:        292     200     252     189      58     152

  Fragments arranged by size: 

                 292     252     200     189     152      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     784    1143 
   Size:        784     359
 
EcoO109I rG'GnC_Cy

Cuts at:      0      56     777    1143 
   Size:         56     721     366

  Fragments arranged by size: 

                 721     366      56
 
EcoRV GAT'ATC

Cuts at:      0     600    1143 
   Size:        600     543
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     681     873     954    1053    1143 
   Size:        681     192      81      99      90

  Fragments arranged by size: 

                 681     192      99      90      81
 
FspI TGC'GCA

Cuts at:      0     232    1143 
   Size:        232     911
 
FspAI rTGC'GCAy

Cuts at:      0     232    1143 
   Size:        232     911
 
HaeII r_GCGC'y

Cuts at:      0     168     268     705     987    1143 
   Size:        168     100     437     282     156

  Fragments arranged by size: 

                 437     282     168     156     100
 
HincII GTy'rAC

Cuts at:      0     843    1143 
   Size:        843     300
 
HindIII A'AGCT_T

Cuts at:      0     340    1143 
   Size:        340     803
 
KasI G'GCGC_C

Cuts at:      0     164    1143 
   Size:        164     979
 
MspA1I CmG'CkG

Cuts at:      0      29      80     444     522     573     693    1143 
   Size:         29      51     364      78      51     120     450

  Fragments arranged by size: 

                 450     364     120      78      51      51      29
 
NaeI GCC'GGC

Cuts at:      0      41      51     431    1143 
   Size:         41      10     380     712

  Fragments arranged by size: 

                 712     380      41      10
 
NarI GG'CG_CC

Cuts at:      0     165    1143 
   Size:        165     978
 
NgoMIV G'CCGG_C

Cuts at:      0      39      49     429    1143 
   Size:         39      10     380     714

  Fragments arranged by size: 

                 714     380      39      10
 
PfoI T'CCnGG_A

Cuts at:      0     545     941    1143 
   Size:        545     396     202

  Fragments arranged by size: 

                 545     396     202
 
PpuMI rG'GwC_Cy

Cuts at:      0     777    1143 
   Size:        777     366
 
PshAI GACnn'nnGTC

Cuts at:      0     838    1143 
   Size:        838     305
 
PspOMI G'GGCC_C

Cuts at:      0      56    1143 
   Size:         56    1087
 
PstI C_TGCA'G

Cuts at:      0    1115    1142    1143 
   Size:       1115      27       1

  Fragments arranged by size: 

                1115      27       1
 
PvuII CAG'CTG

Cuts at:      0     444     522     573    1143 
   Size:        444      78      51     570

  Fragments arranged by size: 

                 570     444      78      51
 
RsrII CG'GwC_CG

Cuts at:      0     159    1143 
   Size:        159     984
 
SapI GCTCTTCn'nnn_

Cuts at:      0     859    1143 
   Size:        859     284
 
SbfI CC_TGCA'GG

Cuts at:      0    1115    1143 
   Size:       1115      28
 
ScaI AGT'ACT

Cuts at:      0     541    1143 
   Size:        541     602
 
SexAI A'CCwGG_T

Cuts at:      0     773     989    1143 
   Size:        773     216     154

  Fragments arranged by size: 

                 773     216     154
 
SfcI C'TryA_G

Cuts at:      0    1111    1138    1143 
   Size:       1111      27       5

  Fragments arranged by size: 

                1111      27       5
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      55    1143 
   Size:         55    1088
 
SfoI GGC'GCC

Cuts at:      0     166    1143 
   Size:        166     977
 
SmlI C'TyrA_G

Cuts at:      0     220     304     312    1143 
   Size:        220      84       8     831

  Fragments arranged by size: 

                 831     220      84       8
 
StyI C'CwwG_G

Cuts at:      0     352     623    1143 
   Size:        352     271     520

  Fragments arranged by size: 

                 520     352     271
 
TatI w'GTAC_w

Cuts at:      0     539    1143 
   Size:        539     604
 
Tth111I GACn'n_nGTC

Cuts at:      0     775    1143 
   Size:        775     368
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     630     747     807    1143 
   Size:        630     117      60     336

  Fragments arranged by size: 

                 630     336     117      60
 
XmnI GAAnn'nnTTC

Cuts at:      0     541     712     826     937    1143 
   Size:        541     171     114     111     206

  Fragments arranged by size: 

                 541     206     171     114     111
 
ZraI GAC'GTC

Cuts at:      0     978    1143 
   Size:        978     165
 

 Enzymes that do cut:

    AatII     AcuI     AfeI    AlwNI     ApaI     BanI    BanII     BbeI
     BbsI    BbvCI     BglI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI     BsmI
    BsmBI Bsp1286I    BsrBI    BsrFI   BssHII    BssSI    BstXI    BstYI
     BtgI     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FspI    FspAI    HaeII   HincII  HindIII     KasI   MspA1I
     NaeI     NarI   NgoMIV     PfoI    PpuMI    PshAI   PspOMI     PstI
    PvuII    RsrII     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SmlI     StyI     TatI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI     BplI    BsaAI    BsaXI     BsgI
  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI    EcoRI     FseI    Hin4I
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI     SacI    SacII     SalI    SanDI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI     XbaI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com