Restriction map for pF1KB3955
 (Linear) (Six-base) MAPSORT of: KIBB3955.seq  Check: 1997  from: 1  to: 1362



KIBB3955.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AatII G_ACGT'C

Cuts at:      0     624    1362 
   Size:        624     738
 
AccI GT'mk_AC

Cuts at:      0    1163    1362 
   Size:       1163     199
 
Acc65I G'GTAC_C

Cuts at:      0     186    1362 
   Size:        186    1176
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1055    1252    1362 
   Size:        919     136     197     110

  Fragments arranged by size: 

                 919     197     136     110
 
AflIII A'CryG_T

Cuts at:      0    1355    1362 
   Size:       1355       7
 
AleI CACnn'nnGTG

Cuts at:      0     430    1362 
   Size:        430     932
 
ApaI G_GGCC'C

Cuts at:      0     823    1362 
   Size:        823     539
 
ApoI r'AATT_y

Cuts at:      0      97     328     862     978    1015    1362 
   Size:         97     231     534     116      37     347

  Fragments arranged by size: 

                 534     347     231     116      97      37
 
BanI G'GyrC_C

Cuts at:      0     186    1362 
   Size:        186    1176
 
BanII G_rGCy'C

Cuts at:      0     823    1362 
   Size:        823     539
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1240    1362 
   Size:       1240     122
 
BclI T'GATC_A

Cuts at:      0     372    1362 
   Size:        372     990
 
BfrBI ATG'CAT

Cuts at:      0     451    1362 
   Size:        451     911
 
BglII A'GATC_T

Cuts at:      0     111     144    1362 
   Size:        111      33    1218

  Fragments arranged by size: 

                1218     111      33
 
Bme1580I G_kGCm'C

Cuts at:      0     823    1362 
   Size:        823     539
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     694    1362 
   Size:        694     668
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1169    1362 
   Size:       1169     193
 
Bpu10I CC'TnA_GC

Cuts at:      0     182    1362 
   Size:        182    1180
 
BsaAI yAC'GTr

Cuts at:      0    1356    1362 
   Size:       1356       6
 
BsaHI Gr'CG_yC

Cuts at:      0     621    1362 
   Size:        621     741
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      48      78    1362 
   Size:         48      30    1284

  Fragments arranged by size: 

                1284      48      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     746    1276    1362 
   Size:        746     530      86

  Fragments arranged by size: 

                 746     530      86
 
BseYI C'CCAG_C

Cuts at:      0     495    1362 
   Size:        495     867
 
BsmI GAATG_Cn'

Cuts at:      0    1058    1362 
   Size:       1058     304
 
Bsp1286I G_dGCh'C

Cuts at:      0     823    1362 
   Size:        823     539
 
BspHI T'CATG_A

Cuts at:      0     120    1362 
   Size:        120    1242
 
BsrGI T'GTAC_A

Cuts at:      0     353    1362 
   Size:        353    1009
 
BstEII G'GTnAC_C

Cuts at:      0      36    1362 
   Size:         36    1326
 
BstYI r'GATC_y

Cuts at:      0     111     144    1362 
   Size:        111      33    1218

  Fragments arranged by size: 

                1218     111      33
 
BtgI C'CryG_G

Cuts at:      0     542     719    1199    1362 
   Size:        542     177     480     163

  Fragments arranged by size: 

                 542     480     177     163
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     497    1362 
   Size:        497     865
 
BtsI GCAGTG_nn'

Cuts at:      0     439     735    1225    1362 
   Size:        439     296     490     137

  Fragments arranged by size: 

                 490     439     296     137
 
DraI TTT'AAA

Cuts at:      0     327    1020    1362 
   Size:        327     693     342

  Fragments arranged by size: 

                 693     342     327
 
EaeI y'GGCC_r

Cuts at:      0     734    1362 
   Size:        734     628
 
EarI CTCTTCn'nnn_

Cuts at:      0     965    1031    1362 
   Size:        965      66     331

  Fragments arranged by size: 

                 965     331      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1055    1169    1252    1362 
   Size:        919     136     114      83     110

  Fragments arranged by size: 

                 919     136     114     110      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     398     819     820     907    1362 
   Size:        398     421       1      87     455

  Fragments arranged by size: 

                 455     421     398      87       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     254    1362 
   Size:        254    1108
 
HindIII A'AGCT_T

Cuts at:      0     744    1362 
   Size:        744     618
 
KpnI G_GTAC'C

Cuts at:      0     190    1362 
   Size:        190    1172
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1362 
   Size:        428     934
 
MslI CAynn'nnrTG

Cuts at:      0     430    1289    1362 
   Size:        430     859      73

  Fragments arranged by size: 

                 859     430      73
 
NcoI C'CATG_G

Cuts at:      0     542     719    1199    1362 
   Size:        542     177     480     163

  Fragments arranged by size: 

                 542     480     177     163
 
NdeI CA'TA_TG

Cuts at:      0     792    1362 
   Size:        792     570
 
NsiI A_TGCA'T

Cuts at:      0     453    1362 
   Size:        453     909
 
NspI r_CATG'y

Cuts at:      0    1311    1362 
   Size:       1311      51
 
PpuMI rG'GwC_Cy

Cuts at:      0     907    1362 
   Size:        907     455
 
PspOMI G'GGCC_C

Cuts at:      0     819    1362 
   Size:        819     543
 
PstI C_TGCA'G

Cuts at:      0     310    1362 
   Size:        310    1052
 
SapI GCTCTTCn'nnn_

Cuts at:      0     965    1362 
   Size:        965     397
 
SfcI C'TryA_G

Cuts at:      0     306    1362 
   Size:        306    1056
 
StyI C'CwwG_G

Cuts at:      0     542     610     719    1199    1362 
   Size:        542      68     109     480     163

  Fragments arranged by size: 

                 542     480     163     109      68
 
TatI w'GTAC_w

Cuts at:      0     353    1362 
   Size:        353    1009
 
XmnI GAAnn'nnTTC

Cuts at:      0     137    1362 
   Size:        137    1225
 
ZraI GAC'GTC

Cuts at:      0     622    1362 
   Size:        622     740
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AleI     ApaI     ApoI
     BanI    BanII     BbsI     BclI    BfrBI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsmI
 Bsp1286I    BspHI    BsrGI   BstEII    BstYI     BtgI    BtgZI     BtsI
     DraI     EaeI     EarI  Eco57MI EcoO109I     FalI  HindIII     KpnI
     MmeI     MslI     NcoI     NdeI     NsiI     NspI    PpuMI   PspOMI
     PstI     SapI     SfcI     StyI     TatI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaWI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     MfeI     MluI     MscI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com