Restriction map for pF1KB3867
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AflIII      
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BsaAI       BsaI        BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspHI       
BspLU11I    BspMI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MluNI       
MscI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          217  GTCTAC               GT^[AC][GT]AC
AccII         678  CGCG                 CG^CG
AccIII        184  TCCGGA               T^CCGGA
AgeI          425  ACCGGT               A^CCGGT
AlwNI         235  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         700  GTGCTC               G[AT]GC[AT]^C
AspI         1009  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         428  GGTCC                G^G[AT]CC
BamHI         635  GGATCC               G^GATCC
BanII         352  GAGCCC               G[GA]GC[CT]^C
BcgI          722  CGAACCGCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         816  AGATCT               A^GATCT
BlpI          681  GCTCAGC              GC^T[ACGT]AGC
BpmI          122  CTGGAG               CTGGAG(16/14)
Bpu1102I      681  GCTCAGC              GC^T[ACGT]AGC
BsaBI         156  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         184  TCCGGA               T^CCGGA
BseRI          22  GAGGAG               GAGGAG(10/8)
BsiHKAI       700  GTGCTC               G[AT]GC[AT]^C
BsmAI         738  GTCTC                GTCTC(1/5)
BspDI         781  ATCGAT               AT^CGAT
BspEI         184  TCCGGA               T^CCGGA
BsrFI         425  ACCGGT               [GA]^CCGG[CT]
BssSI         733  CACGAG               CACGAG(-5/-1)
BstUI         678  CGCG                 CG^CG
BstXI         168  CCATGAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         681  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        425  ACCGGT               [GA]^CCGG[CT]
ClaI          781  ATCGAT               AT^CGAT
DraII         876  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          260  CCATGG               C^C[GA][CT]GG
EaeI          659  CGGCCA               [CT]^GGCC[GA]
EcoNI          66  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      876  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       260  CCATGG               C^C[AT][AT]GG
HaeII          85  AGCGCC               [GA]GCGC^[CT]
MamI          156  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          184  TCCGGA               T^CCGGA
MspA1I        684  CAGCGG               C[AC]G^C[GT]G
MvnI          678  CGCG                 CG^CG
NcoI          260  CCATGG               C^CATGG
NspI          728  GCATGC               [GA]CATG^[CT]
PaeR7I         37  CTCGAG               C^TCGAG
PflFI        1009  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         234  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         425  ACCGGT               A^CCGGT
PstI          362  CTGCAG               CTGCA^G
SapI          747  GCTCTTC              GCTCTTC(1/4)
SexAI         695  ACCAGGT              A^CC[AT]GGT
SmaI          431  CCCGGG               CCC^GGG
SmlI           37  CTCGAG               C^T[CT][GA]AG
SphI          728  GCATGC               GCATG^C
StyI          260  CCATGG               C^C[AT][AT]GG
Tth111I      1009  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        234  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           37  CTCGAG               C^TCGAG
XmaCI         431  CCCGGG               C^CCGGG
XmaI          431  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AatII         601  GACGTC               GACGT^C
              652  GACGTC              
AluI          368  AGCT                 AG^CT
              443  AGCT                
BmyI          352  GAGCCC               G[AGT]GC[ACT]^C
              700  GTGCTC              
BsaWI         184  TCCGGA               [AT]^CCGG[AT]
              425  ACCGGT              
BsmFI         237  GTCCC                GGGAC(10/14)
              429  GTCCC               
Bsp1286I      352  GAGCCC               G[AGT]GC[ACT]^C
              700  GTGCTC              
BsrBI         936  CCGCTC               CCGCTC(-3/-3)
               40  GAGCGG              
BsrDI         640  CATTGC               GCAATG(2/0)
             1029  CATTGC              
BsrI          547  ACTGG                ACTGG(1/-1)
              234  CCAGT               
BstYI         635  GGATCC               [GA]^GATC[CT]
              816  AGATCT              
Cac8I         611  GCCAGC               GC[ACGT]^[ACGT]GC
              728  GCATGC              
EarI          748  CTCTTC               CTCTTC(1/4)
              799  CTCTTC              
Ksp632I       748  CTCTTC               CTCTTC(1/4)
              799  CTCTTC              
MflI          635  GGATCC               [GA]^GATC[CT]
              816  AGATCT              
MwoI          507  GCCCACTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              929  GCAAAAACCGC         
SfcI          219  CTACAG               C^T[GA][CT]AG
              362  CTGCAG              
TfiI          127  GAATC                G^A[AT]TC
              407  GAATC               
Tsp509I       104  AATT                 ^AATT
              920  AATT                
TspRI         510  CACTG                CA[GC]TG[ACGT][ACGT]^
              546  CACTG               
XcmI          260  CCATGGACACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              458  CCAAATACTACCTGG     
XhoII         635  GGATCC               [GA]^GATC[CT]
              816  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcyI        AfaI        AlwI        AvaI        
BbsI        BbvI        BcnI        BpuAI       BsaHI       
BsaJI       BsiYI       BslI        BsoBI       BssKI       
BstF5I      BstNI       CfoI        Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspI        MvaI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TseI        Tsp45I      
TthHB8I     
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