Restriction map for pF1KB3758
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AfaI        
AflII       AflIII      AgeI        AhdI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BbrPI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MseI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII         517  GACGTC               GACGT^C
AccIII        102  TCCGGA               T^CCGGA
Alw44I         74  GTGCAC               G^TGCAC
AocI         1326  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      1187  AGCGCT               AGC^GCT
ApaLI          74  GTGCAC               G^TGCAC
AviII         114  TGCGCA               TGC^GCA
BalI         1661  TGGCCA               TGG^CCA
BamHI         151  GGATCC               G^GATCC
BbeI          242  GGCGCC               GGCGC^C
BsaI         1531  GAGACC               GGTCTC(1/5)
BseAI         102  TCCGGA               T^CCGGA
BsgI          916  CTGCAC               GTGCAG(16/14)
BsmI          253  GCATTC               GAATGC(1/-1)
BspEI         102  TCCGGA               T^CCGGA
BsrBI          98  CCGCTC               CCGCTC(-3/-3)
BsrDI         911  CATTGC               GCAATG(2/0)
BssHII        438  GCGCGC               G^CGCGC
BstYI         151  GGATCC               [GA]^GATC[CT]
Bsu36I       1326  CCTAAGG              CC^T[ACGT]AGG
DraIII        730  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1187  AGCGCT               AGC^GCT
Eco81I       1326  CCTAAGG              CC^T[ACGT]AGG
EcoRV        1408  GATATC               GAT^ATC
FspI          114  TGCGCA               TGC^GCA
KasI          242  GGCGCC               G^GCGCC
MflI          151  GGATCC               [GA]^GATC[CT]
MluNI        1661  TGGCCA               TGG^CCA
MroI          102  TCCGGA               T^CCGGA
MscI         1661  TGGCCA               TGG^CCA
MslI          874  CATCCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          242  GGCGCC               GG^CGCC
PleI         1203  GACTC                GAGTC(4/5)
SexAI         731  ACCAGGT              A^CC[AT]GGT
SfoI          242  GGCGCC               GGC^GCC
SmlI         1021  CTTGAG               C^T[CT][GA]AG
StuI         1001  AGGCCT               AGG^CCT
Tsp45I       1591  GTGAC                ^GT[GC]AC
XhoII         151  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

ApaI         1620  GGGCCC               GGGCC^C
             1736  GGGCCC              
BglI          211  GCCTCGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1600  GCCCTGGAGGC         
BlpI          698  GCTCAGC              GC^T[ACGT]AGC
             1090  GCTGAGC             
Bpu1102I      698  GCTCAGC              GC^T[ACGT]AGC
             1090  GCTGAGC             
BsmBI        1247  CGTCTC               CGTCTC(1/5)
             1222  GAGACG              
BspMI         860  ACCTGC               ACCTGC(4/8)
             1362  GCAGGT              
BstXI         556  CCACCTGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1610  CCAGCGGCTTGG        
CelII         698  GCTCAGC              GC^T[ACGT]AGC
             1090  GCTGAGC             
DsaI          307  CCGTGG               C^C[GA][CT]GG
             1731  CCACGG              
HaeII         242  GGCGCC               [GA]GCGC^[CT]
             1187  AGCGCT              
MaeIII        574  GTTAC                ^GT[ACGT]AC
             1591  GTGAC               
NlaIII       1413  CATG                 CATG^
             1638  CATG                
PpuMI          53  GGGACCC              [GA]G^G[AT]CC[CT]
              605  GGGACCT             
TaqI         1013  TCGA                 T^CGA
             1356  TCGA                
TfiI          772  GATTC                G^A[AT]TC
              981  GAATC               
TthHB8I      1013  TCGA                 T^CGA
             1356  TCGA                
XcmI          327  CCAAGGGCCGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1076  CCAGCCAACAGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIV       
PflMI       PstI        PvuII       SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TseI        Tsp509I     TspRI       Van91I      
XmaCI       XmaI        
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