Restriction map for pF1KB3711
##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MspI        
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AfaI          215  GTAC                 GT^AC
AlwI          154  GATCC                GGATC(4/5)
AlwNI         170  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       201  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           88  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         117  GGACC                G^G[AT]CC
BbrPI         142  CACGTG               CAC^GTG
BfaI           32  CTAG                 C^TAG
BsaAI         142  CACGTG               [CT]AC^GT[GA]
BsaI           99  GGTCTC               GGTCTC(1/5)
BsiYI          41  CCCAAGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           41  CCCAAGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         100  GTCTC                GTCTC(1/5)
BsrI          169  CCAGT                ACTGG(1/-1)
BstYI         153  AGATCC               [GA]^GATC[CT]
Cac8I         174  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I        117  GGACC                G^G[ACGT]CC
DpnII         154  GATC                 ^GATC
DsaI          113  CCGCGG               C^C[GA][CT]GG
FauI          147  GCGGG                CCCGC(4/6)
HaeIII        126  GGCC                 GG^CC
KspI          113  CCGCGG               CCGC^GG
MaeI           32  CTAG                 C^TAG
MaeII         143  ACGT                 A^CGT
MboI          154  GATC                 ^GATC
MflI          153  AGATCC               [GA]^GATC[CT]
MslI          165  CATTCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        113  CCGCGG               C[AC]G^C[GT]G
NdeII         154  GATC                 ^GATC
NlaIII         12  CATG                 CATG^
NruI          195  TCGCGA               TCG^CGA
NspI           11  GCATGT               [GA]CATG^[CT]
PflFI          88  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         142  CACGTG               CAC^GTG
PmlI          142  CACGTG               CAC^GTG
RsaI          215  GTAC                 GT^AC
SacII         113  CCGCGG               CCGC^GG
Sau3AI        154  GATC                 ^GATC
Sau96I        117  GGACC                G^G[ACGT]CC
TspRI         170  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        88  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           47  CCAGGAAAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         153  AGATCC               [GA]^GATC[CT]
XmnI          201  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AccII         114  CGCG                 CG^CG
              196  CGCG                
BbvI           63  GCAGC                GCAGC(8/12)
              109  GCAGC               
BsaJI         104  CCCAGG               C^C[ACGT][ACGT]GG
              113  CCGCGG              
BstUI         114  CGCG                 CG^CG
              196  CGCG                
MboII         151  GAAGA                GAAGA(8/7)
              201  GAAGA               
MvnI          114  CGCG                 CG^CG
              196  CGCG                
MwoI           26  GCACCACTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               46  GCCAGGAAAGC         
SfaNI          35  GCATC                GCATC(5/9)
              178  GCATC               
TseI           63  GCAGC                G^C[AT]GC
              109  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AciI        AluI        BssKI       BstNI       EcoRII      
Fnu4HI      ItaI        MnlI        MvaI        ScrFI       
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