Restriction map for pF1KB3544
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII          55  CGCG                 CG^CG
AciI           56  GCGG                 CCGC(-3/-1)
AcsI          106  AAATTT               [GA]^AATT[CT]
AluI          308  AGCT                 AG^CT
Alw44I         73  GTGCAC               G^TGCAC
AlwI          168  GATCC                GGATC(4/5)
ApaLI          73  GTGCAC               G^TGCAC
ApoI          106  AAATTT               [GA]^AATT[CT]
AspHI          73  GTGCAC               G[AT]GC[AT]^C
AvaI          119  CCCGGG               C^[CT]CG[GA]G
BbsI          295  GTCTTC               GAAGAC(2/6)
BfaI          151  CTAG                 C^TAG
BpmI          233  CTCCAG               CTGGAG(16/14)
BpuAI         295  GTCTTC               GAAGAC(2/6)
BsgI            7  GTGCAG               GTGCAG(16/14)
BsiHKAI        73  GTGCAC               G[AT]GC[AT]^C
BsmAI          32  GAGAC                GTCTC(1/5)
BsmBI          32  GAGACG               CGTCTC(1/5)
BsmFI         122  GGGAC                GGGAC(10/14)
BsoBI         119  CCCGGG               C^[CT]CG[GA]G
BstUI          55  CGCG                 CG^CG
Cac8I         155  GCAAGC               GC[ACGT]^[ACGT]GC
DpnII         168  GATC                 ^GATC
EaeI           57  CGGCCA               [CT]^GGCC[GA]
Fnu4HI         56  GCGGC                GC^[ACGT]GC
HaeIII         58  GGCC                 GG^CC
HinfI         112  GATTC                G^A[ACGT]TC
HphI          165  GGTGA                GGTGA(8/7)
ItaI           56  GCGGC                GC^[ACGT]GC
MaeI          151  CTAG                 C^TAG
MboI          168  GATC                 ^GATC
MseI          140  TTAA                 T^TAA
MslI           81  CACCGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           55  CGCG                 CG^CG
NdeII         168  GATC                 ^GATC
NlaIII        283  CATG                 CATG^
NspI          282  ACATGC               [GA]CATG^[CT]
Sau3AI        168  GATC                 ^GATC
SmaI          119  CCCGGG               CCC^GGG
TaqI          299  TCGA                 T^CGA
TfiI          112  GATTC                G^A[AT]TC
Tru9I         140  TTAA                 T^TAA
Tsp509I       107  AATT                 ^AATT
TspRI         255  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       299  TCGA                 T^CGA
XmaCI         119  CCCGGG               C^CCGGG
XmaI          119  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BanI          250  GGTGCC               G^G[CT][GA]CC
              260  GGCACC              
BmyI           73  GTGCAC               G[AGT]GC[ACT]^C
              259  GGGCAC              
Bsp1286I       73  GTGCAC               G[AGT]GC[ACT]^C
              259  GGGCAC              
BspMI          64  ACCTGC               ACCTGC(4/8)
                9  GCAGGT              
BsrI          256  ACTGG                ACTGG(1/-1)
              318  ACTGG               
BstF5I        273  CATCC                GGATG(2/0)
               86  GGATG               
BstNI          28  CCAGG                CC^[AT]GG
              265  CCAGG               
CfoI           39  GCGC                 GCG^C
               54  GCGC                
EcoRII         28  CCAGG                ^CC[AT]GG
              265  CCAGG               
FokI          273  CATCC                GGATG(9/13)
               86  GGATG               
HapII          83  CCGG                 C^CGG
              120  CCGG                
HhaI           39  GCGC                 GCG^C
               54  GCGC                
HinP1I         39  GCGC                 G^CGC
               54  GCGC                
HpaII          83  CCGG                 C^CGG
              120  CCGG                
MspI           83  CCGG                 C^CGG
              120  CCGG                
MvaI           28  CCAGG                CC^[AT]GG
              265  CCAGG               
NlaIV         250  GGTGCC               GG[ACGT]^[ACGT]CC
              260  GGCACC              
SfaNI          87  GATGC                GCATC(5/9)
              269  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

BcnI        BsaJI       BsiYI       BslI        BssKI       
MboII       MnlI        MwoI        NciI        ScrFI       
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