Restriction map for pF1KB3441
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AvaI        
AvrII       BamHI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BlnI        BlpI        
Bpu1102I    BpuAI       BseAI       BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DsaI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       EcoT14I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     KspI        MaeI        MfeI        MluI        
MroI        MunI        NcoI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        StyI        SwaI        Tsp509I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AfaI          864  GTAC                 GT^AC
AflII          40  CTTAAG               C^TTAAG
AflIII        493  ACGTGT               A^C[GA][CT]GT
AhdI          590  GACTGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          815  GGATC                GGATC(4/5)
AspEI         590  GACTGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         778  GAGCTC               G[AT]GC[AT]^C
AspI          984  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         134  TGCGCA               TGC^GCA
BanII         778  GAGCTC               G[GA]GC[CT]^C
BbeI          845  GGCGCC               GGCGC^C
BbrPI         492  CACGTG               CAC^GTG
BcnI          909  CCGGG                CC^[GC]GG
BfrI           40  CTTAAG               C^TTAAG
BglI          904  GCCGACCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         492  CACGTG               [CT]AC^GT[GA]
BsaBI         457  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          514  GAGACC               GGTCTC(1/5)
BsaWI         541  ACCGGA               [AT]^CCGG[AT]
BsgI           74  GTGCAG               GTGCAG(16/14)
BsiEI         906  CGACCG               CG[GA][CT]^CG
BsiHKAI       778  GAGCTC               G[AT]GC[AT]^C
BsrBI         840  GAGCGG               CCGCTC(-3/-3)
BsrDI         713  GCAATG               GCAATG(2/0)
BstEII        430  GGTTACC              G^GT[ACGT]ACC
BstPI         430  GGTTACC              G^GT[ACGT]ACC
DraII         988  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        489  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          934  GACAACCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      590  GACTGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      988  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       430  GGTTACC              G^GT[ACGT]ACC
EcoT22I       716  ATGCAT               ATGCA^T
FspI          134  TGCGCA               TGC^GCA
HaeII         845  GGCGCC               [GA]GCGC^[CT]
KasI          845  GGCGCC               G^GCGCC
MamI          457  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         122  GAAGA                GAAGA(8/7)
MseI           41  TTAA                 T^TAA
MslI          972  CATGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        945  CAGCTG               C[AC]G^C[GT]G
NaeI          653  GCCGGC               GCC^GGC
NarI          845  GGCGCC               GG^CGCC
NciI          909  CCGGG                CC^[GC]GG
NdeI          311  CATATG               CA^TATG
NgoMIV        653  GCCGGC               G^CCGGC
NsiI          716  ATGCAT               ATGCA^T
PflFI         984  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         492  CACGTG               CAC^GTG
PmlI          492  CACGTG               CAC^GTG
PpuMI         988  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          747  CTGCAG               CTGCA^G
PvuII         945  CAGCTG               CAG^CTG
RsaI          864  GTAC                 GT^AC
SacI          778  GAGCTC               GAGCT^C
SbfI          746  CCTGCAGG             CCTGCA^GG
SfcI          747  CTGCAG               C^T[GA][CT]AG
SfoI          845  GGCGCC               GGC^GCC
Sse8387I      746  CCTGCAGG             CCTGCA^GG
Tru9I          41  TTAA                 T^TAA
Tth111I       984  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         400  CGCG                 CG^CG
              940  CGCG                
AvaII         267  GGTCC                G^G[AT]CC
              989  GGTCC               
BalI          164  TGGCCA               TGG^CCA
              669  TGGCCA              
BanI          158  GGCACC               G^G[CT][GA]CC
              845  GGCGCC              
BbvI          443  GCTGC                GCAGC(8/12)
              947  GCTGC               
BglII         170  AGATCT               A^GATCT
             1010  AGATCT              
BsmBI         597  CGTCTC               CGTCTC(1/5)
               56  GAGACG              
BsmFI         245  GGGAC                GGGAC(10/14)
              506  GGGAC               
BspMI         745  ACCTGC               ACCTGC(4/8)
              913  GCAGGT              
BsrFI         517  ACCGGC               [GA]^CCGG[CT]
              653  GCCGGC              
BstUI         400  CGCG                 CG^CG
              940  CGCG                
BstYI         170  AGATCT               [GA]^GATC[CT]
             1010  AGATCT              
Cfr10I        517  ACCGGC               [GA]^CCGG[CT]
              653  GCCGGC              
Cfr13I        267  GGTCC                G^G[ACGT]CC
              989  GGTCC               
DdeI           92  CTCAG                C^T[ACGT]AG
              732  CTCAG               
Fnu4HI        443  GCTGC                GC^[ACGT]GC
              947  GCTGC               
HphI          473  TCACC                GGTGA(8/7)
              818  TCACC               
ItaI          443  GCTGC                GC^[ACGT]GC
              947  GCTGC               
MflI          170  AGATCT               [GA]^GATC[CT]
             1010  AGATCT              
MluNI         164  TGGCCA               TGG^CCA
              669  TGGCCA              
MscI          164  TGGCCA               TGG^CCA
              669  TGGCCA              
MvnI          400  CGCG                 CG^CG
              940  CGCG                
NspI           70  GCATGT               [GA]CATG^[CT]
              647  GCATGT              
PflMI         179  CCATGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              623  CCAAACTCTGG         
Sau96I        267  GGTCC                G^G[ACGT]CC
              989  GGTCC               
SmlI           40  CTTAAG               C^T[CT][GA]AG
             1006  CTCAAG              
TseI          443  GCTGC                G^C[AT]GC
              947  GCTGC               
Tsp45I        472  GTCAC                ^GT[GC]AC
              958  GTGAC               
Van91I        179  CCATGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              623  CCAAACTCTGG         
XhoII         170  AGATCT               [GA]^GATC[CT]
             1010  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AcyI        AluI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BspHI       BsrI        BssKI       
BssSI       BstF5I      BstNI       Cac8I       CfoI        
DpnII       EaeI        EcoRII      FauI        FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       MaeII       MaeIII      
MboI        MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RcaI        
Sau3AI      ScrFI       SfaNI       TaqI        TfiI        
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com