Restriction map for pF1KB3441
 (Circular) (Six-base) MAPSORT of: pF1KB3441.seq  Check: 2463  from: 1  to: 4133



pF1KB3441  4133 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    862    1012     862 
   Size:        150    3983
 
AccI GT'mk_AC

Cuts at:   1171    1171 
   Size:       4133
 
Acc65I G'GTAC_C

Cuts at:   1149    1149 
   Size:       4133
 
AclI AA'CG_TT

Cuts at:   1127    3973    1127 
   Size:       2846    1287
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    710    1958    2390    3137    3405     710 
   Size:       1248     432     747     268    1438

  Fragments arranged by size: 

                1438    1248     747     432     268
 
AflII C'TTAA_G

Cuts at:    148     148 
   Size:       4133
 
AflIII A'CryG_T

Cuts at:    601    1485    2589    3566     601 
   Size:        884    1104     977    1168

  Fragments arranged by size: 

                1168    1104     977     884
 
AgeI A'CCGG_T

Cuts at:   2521    2521 
   Size:       4133
 
AhdI GACnn_n'nnGTC

Cuts at:    703     703 
   Size:       4133
 
AlwNI CAG_nnn'CTG

Cuts at:   1438    3005    1438 
   Size:       1567    2566
 
ApaLI G'TGCA_C

Cuts at:   2903    2903 
   Size:       4133
 
ApoI r'AATT_y

Cuts at:   1137    3486    3742    1137 
   Size:       2349     256    1528

  Fragments arranged by size: 

                2349    1528     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4133
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4133
 
AvaI C'yCGr_G

Cuts at:   1153    1153 
   Size:       4133
 
BamHI G'GATC_C

Cuts at:   1158    1158 
   Size:       4133
 
BanI G'GyrC_C

Cuts at:    266     953    1149    1563    1811    1846     266 
   Size:        687     196     414     248      35    2553

  Fragments arranged by size: 

                2553     687     414     248     196      35
 
BanII G_rGCy'C

Cuts at:    890    1147    2177     890 
   Size:        257    1030    2846

  Fragments arranged by size: 

                2846    1030     257
 
BbeI G_GCGC'C

Cuts at:    957    1567    1815     957 
   Size:        610     248    3275

  Fragments arranged by size: 

                3275     610     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2031    2792    4133    2031 
   Size:        761    1341    2031

  Fragments arranged by size: 

                2031    1341     761
 
BclI T'GATC_A

Cuts at:   1653    1653 
   Size:       4133
 
BfrBI ATG'CAT

Cuts at:    826     826 
   Size:       4133
 
BglI GCCn_nnn'nGGC

Cuts at:   1018    1018 
   Size:       4133
 
BglII A'GATC_T

Cuts at:    278    1118    1648    3685     278 
   Size:        840     530    2037     726

  Fragments arranged by size: 

                2037     840     726     530
 
BlpI GC'TnA_GC

Cuts at:   1245    1245 
   Size:       4133
 
Bme1580I G_kGCm'C

Cuts at:    269     301     609    1758    1851    2907     269 
   Size:         32     308    1149      93    1056    1495

  Fragments arranged by size: 

                1495    1149    1056     308      93      32
 
BmrI ACTGGGnnnn_n'

Cuts at:    217     303    1345    1426    1761    3905     217 
   Size:         86    1042      81     335    2144     445

  Fragments arranged by size: 

                2144    1042     445     335      86      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    695    2500     695 
   Size:       1805    2328
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    291     611     680     291 
   Size:        320      69    3744

  Fragments arranged by size: 

                3744     320      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1099    1309    2188    2680    2978    1099 
   Size:        210     879     492     298    2254

  Fragments arranged by size: 

                2254     879     492     298     210
 
BsaI GGTCTCn'nnnn_

Cuts at:    616    3837     616 
   Size:       3221     912
 
BsaAI yAC'GTr

Cuts at:    602    1486     602 
   Size:        884    3249
 
BsaBI GATnn'nnATC

Cuts at:      6     569    1647    3684       6 
   Size:        563    1078    2037     455

  Fragments arranged by size: 

                2037    1078     563     455
 
BsaHI Gr'CG_yC

Cuts at:    167     848     954    1564    1812    2514    4010     167 
   Size:        681     106     610     248     702    1496     290

  Fragments arranged by size: 

                1496     702     681     610     290     248     106
 
BsaWI w'CCGG_w

Cuts at:    649    1322    1843    2521    2795    2942    3353     649 
   Size:        673     521     678     274     147     411    1429

  Fragments arranged by size: 

                1429     678     673     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    454     484     454 
   Size:         30    4103
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    264     387     949     264 
   Size:        123     562    3448

  Fragments arranged by size: 

                3448     562     123
 
BseYI C'CCAG_C

Cuts at:    967    1033    1558    2316    2893    3389     967 
   Size:         66     525     758     577     496    1711

  Fragments arranged by size: 

                1711     758     577     525     496      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    203     203 
   Size:       4133
 
BsiEI CG_ry'CG

Cuts at:    104    1017    1675    1721    2929     104 
   Size:        913     658      46    1208    1308

  Fragments arranged by size: 

                1308    1208     913     658      46
 
BsiHKAI G_wGCw'C

Cuts at:    890    1147    1925    2115    2907     890 
   Size:        257     778     190     792    2116

  Fragments arranged by size: 

                2116     792     778     257     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    158     711     158 
   Size:        553    3580
 
Bsp1286I G_dGCh'C

Cuts at:    269     301     609     890    1147    1758    1851    1925    2115 
   Size:         32     308     281     257     611      93      74     190

Cuts at:   2115    2177    2907     269 
   Size:         62     730    1495

  Fragments arranged by size: 

                1495     730     611     308     281     257     190      93
                  74      62      32
 
BspEI T'CCGG_A

Cuts at:   3353    3353 
   Size:       4133
 
BspHI T'CATG_A

Cuts at:    407     668     890    3309     407 
   Size:        261     222    2419    1231

  Fragments arranged by size: 

                2419    1231     261     222
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    862    1012    1183    1397    1699    2080     862 
   Size:        150     171     214     302     381    2915

  Fragments arranged by size: 

                2915     381     302     214     171     150
 
BsrBI CCG'CTC

Cuts at:     50     950    2425    2479    3963    4128      50 
   Size:        900    1475      54    1484     165      55

  Fragments arranged by size: 

                1484    1475     900     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    828    2045     828 
   Size:       1217    2916
 
BsrFI r'CCGG_y

Cuts at:    625     761    2131    2312    2521     625 
   Size:        136    1370     181     209    2237

  Fragments arranged by size: 

                2237    1370     209     181     136
 
BssSI C'ACGA_G

Cuts at:    246     491     781    2404    2762     246 
   Size:        245     290    1623     358    1617

  Fragments arranged by size: 

                1623    1617     358     290     245
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1510    1510 
   Size:       4133
 
BstBI TT'CG_AA

Cuts at:   2494    2494 
   Size:       4133
 
BstEII G'GTnAC_C

Cuts at:    538     538 
   Size:       4133
 
BstYI r'GATC_y

Cuts at:      1     278    1118    1158    1625    1648    1983    2229    3241 
   Size:        277     840      40     467      23     335     246    1012

Cuts at:   3241    3327    3339    3685       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     840     467     449     346     335     277     246
                  86      40      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2083    2264    2427    2083 
   Size:        181     163    3789

  Fragments arranged by size: 

                3789     181     163
 
BtsI GCAGTG_nn'

Cuts at:    256    1376     256 
   Size:       1120    3013
 
DraI TTT'AAA

Cuts at:   1132    1132 
   Size:       4133
 
DraIII CAC_nnn'GTG

Cuts at:    602     602 
   Size:       4133
 
DrdI GACnn_nn'nnGTC

Cuts at:   1048    1839    2697    1048 
   Size:        791     858    2484

  Fragments arranged by size: 

                2484     858     791
 
EaeI y'GGCC_r

Cuts at:    272     627     777    1718    1892    2283    2310    3655     272 
   Size:        355     150     941     174     391      27    1345     750

  Fragments arranged by size: 

                1345     941     750     391     355     174     150      27
            
 
EagI C'GGCC_G

Cuts at:   1718    1718 
   Size:       4133
 
EarI CTCTTCn'nnn_

Cuts at:   2156    2366    2156 
   Size:        210    3923
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2341    2651    2797    3943    2341 
   Size:        310     146    1146    2531

  Fragments arranged by size: 

                2531    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    888    1145     888 
   Size:        257    3876
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    291     611     680     710    1958    2390    3137    3405     291 
   Size:        320      69      30    1248     432     747     268    1019

  Fragments arranged by size: 

                1248    1019     747     432     320     268      69      30
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1097    1272    1097 
   Size:        175    3958
 
EcoRI G'AATT_C

Cuts at:   1137    1137 
   Size:       4133
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2152    2152 
   Size:       4133
 
FspI TGC'GCA

Cuts at:    244    1914     244 
   Size:       1670    2463
 
FspAI rTGC'GCAy

Cuts at:    244     244 
   Size:       4133
 
HaeII r_GCGC'y

Cuts at:    957    1567    1815    2837    3816     957 
   Size:        610     248    1022     979    1274

  Fragments arranged by size: 

                1274    1022     979     610     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    689     721    1975    2005    2007    2037     689 
   Size:         32    1254      30       2      30    2785

  Fragments arranged by size: 

                2785    1254      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1172    1172 
   Size:       4133
 
KasI G'GCGC_C

Cuts at:    953    1563    1811     953 
   Size:        610     248    3275

  Fragments arranged by size: 

                3275     610     248
 
KpnI G_GTAC'C

Cuts at:   1153    1153 
   Size:       4133
 
MluI A'CGCG_T

Cuts at:   3566    3566 
   Size:       4133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2804    2988    2804 
   Size:        184    3949
 
MscI TGG'CCA

Cuts at:    274     779    1894     274 
   Size:        505    1115    2513

  Fragments arranged by size: 

                2513    1115     505
 
MslI CAynn'nnrTG

Cuts at:   1084    2122    2249    1084 
   Size:       1038     127    2968

  Fragments arranged by size: 

                2968    1038     127
 
MspA1I CmG'CkG

Cuts at:   1055    1244    1558    1918    2931    3176    1055 
   Size:        189     314     360    1013     245    2012

  Fragments arranged by size: 

                2012    1013     360     314     245     189
 
NaeI GCC'GGC

Cuts at:    763    2314     763 
   Size:       1551    2582
 
NarI GG'CG_CC

Cuts at:    954    1564    1812     954 
   Size:        610     248    3275

  Fragments arranged by size: 

                3275     610     248
 
NdeI CA'TA_TG

Cuts at:    420     420 
   Size:       4133
 
NgoMIV G'CCGG_C

Cuts at:    761    2312     761 
   Size:       1551    2582
 
NsiI A_TGCA'T

Cuts at:    828     828 
   Size:       4133
 
NspI r_CATG'y

Cuts at:    182     759    1186    1686    2593     182 
   Size:        577     427     500     907    1722

  Fragments arranged by size: 

                1722     907     577     500     427
 
PciI A'CATG_T

Cuts at:   2589    2589 
   Size:       4133
 
PflMI CCAn_nnn'nTGG

Cuts at:    293     737     293 
   Size:        444    3689
 
PfoI T'CCnGG_A

Cuts at:    560     560 
   Size:       4133
 
PmeI GTTT'AAAC

Cuts at:   1132    1132 
   Size:       4133
 
PmlI CAC'GTG

Cuts at:    602     602 
   Size:       4133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3272    3304    3272 
   Size:         32    4101
 
PpuMI rG'GwC_Cy

Cuts at:   1097    1097 
   Size:       4133
 
PstI C_TGCA'G

Cuts at:    859    1180    1865     859 
   Size:        321     685    3127

  Fragments arranged by size: 

                3127     685     321
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4133
 
PvuII CAG'CTG

Cuts at:   1055    1558    1918    1055 
   Size:        503     360    3270

  Fragments arranged by size: 

                3270     503     360
 
RsrII CG'GwC_CG

Cuts at:   2328    2328 
   Size:       4133
 
SacI G_AGCT'C

Cuts at:    890    1147     890 
   Size:        257    3876
 
SalI G'TCGA_C

Cuts at:   1170    1170 
   Size:       4133
 
SapI GCTCTTCn'nnn_

Cuts at:   2156    2366    2156 
   Size:        210    3923
 
SbfI CC_TGCA'GG

Cuts at:    859    1180     859 
   Size:        321    3812
 
ScaI AGT'ACT

Cuts at:   3650    3650 
   Size:       4133
 
SfcI C'TryA_G

Cuts at:     33     855    1176    1861    2854    3045      33 
   Size:        822     321     685     993     191    1121

  Fragments arranged by size: 

                1121     993     822     685     321     191
 
SfoI GGC'GCC

Cuts at:    955    1565    1813     955 
   Size:        610     248    3275

  Fragments arranged by size: 

                3275     610     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4133
 
SmaI CCC'GGG

Cuts at:   1155    1155 
   Size:       4133
 
SmlI C'TyrA_G

Cuts at:    148    1114    1288    2203    2695    2957     148 
   Size:        966     174     915     492     262    1324

  Fragments arranged by size: 

                1324     966     915     492     262     174
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4133
 
SphI G_CATG'C

Cuts at:   1186    1686    1186 
   Size:        500    3633
 
StyI C'CwwG_G

Cuts at:   1267    1621    1267 
   Size:        354    3779
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1716    1849    2308    2517    1716 
   Size:        133     459     209    3332

  Fragments arranged by size: 

                3332     459     209     133
 
TatI w'GTAC_w

Cuts at:   3648    3648 
   Size:       4133
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    165     197    3650    3682     165 
   Size:         32    3453      32     616

  Fragments arranged by size: 

                3453     616      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1095    1930    1095 
   Size:        835    3298
 
XbaI T'CTAG_A

Cuts at:   1164    1164 
   Size:       4133
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1628    1628 
   Size:       4133
 
XmaI C'CCGG_G

Cuts at:   1153    1153 
   Size:       4133
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NsiI     NspI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AleI     AlfI     AloI     ApaI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BmgBI     BmtI   Bpu10I    BsiWI
     BsmI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI    EcoNI    EcoRV     FseI  HindIII     HpaI     MfeI     NcoI
     NheI     NotI     NruI     PacI     PasI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com