Restriction map for pF1KB3441
 (Linear) (Six-base) MAPSORT of: KIBB3441.seq  Check: 5051  from: 1  to: 1020



KIBB3441.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     754     904    1020 
   Size:        754     150     116

  Fragments arranged by size: 

                 754     150     116
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602    1020 
   Size:        602     418
 
AflII C'TTAA_G

Cuts at:      0      40    1020 
   Size:         40     980
 
AflIII A'CryG_T

Cuts at:      0     493    1020 
   Size:        493     527
 
AhdI GACnn_n'nnGTC

Cuts at:      0     595    1020 
   Size:        595     425
 
BanI G'GyrC_C

Cuts at:      0     158     845    1020 
   Size:        158     687     175

  Fragments arranged by size: 

                 687     175     158
 
BanII G_rGCy'C

Cuts at:      0     782    1020 
   Size:        782     238
 
BbeI G_GCGC'C

Cuts at:      0     849    1020 
   Size:        849     171
 
BfrBI ATG'CAT

Cuts at:      0     718    1020 
   Size:        718     302
 
BglI GCCn_nnn'nGGC

Cuts at:      0     910    1020 
   Size:        910     110
 
BglII A'GATC_T

Cuts at:      0     170    1010    1020 
   Size:        170     840      10

  Fragments arranged by size: 

                 840     170      10
 
Bme1580I G_kGCm'C

Cuts at:      0     161     193     501    1020 
   Size:        161      32     308     519

  Fragments arranged by size: 

                 519     308     161      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     109     195    1020 
   Size:        109      86     825

  Fragments arranged by size: 

                 825     109      86
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     587    1020 
   Size:        587     433
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     503     572    1020 
   Size:        183     320      69     448

  Fragments arranged by size: 

                 448     320     183      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    1020 
   Size:        991      29
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     508    1020 
   Size:        508     512
 
BsaAI yAC'GTr

Cuts at:      0     494    1020 
   Size:        494     526
 
BsaBI GATnn'nnATC

Cuts at:      0     461    1020 
   Size:        461     559
 
BsaHI Gr'CG_yC

Cuts at:      0      59     740     846    1020 
   Size:         59     681     106     174

  Fragments arranged by size: 

                 681     174     106      59
 
BsaWI w'CCGG_w

Cuts at:      0     541    1020 
   Size:        541     479
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     346     376    1020 
   Size:        346      30     644

  Fragments arranged by size: 

                 644     346      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     279     841    1020 
   Size:        156     123     562     179

  Fragments arranged by size: 

                 562     179     156     123
 
BseYI C'CCAG_C

Cuts at:      0     859     925    1020 
   Size:        859      66      95

  Fragments arranged by size: 

                 859      95      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95    1020 
   Size:         95     925
 
BsiEI CG_ry'CG

Cuts at:      0     909    1020 
   Size:        909     111
 
BsiHKAI G_wGCw'C

Cuts at:      0     782    1020 
   Size:        782     238
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      50     603    1020 
   Size:         50     553     417

  Fragments arranged by size: 

                 553     417      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     193     501     782    1020 
   Size:        161      32     308     281     238

  Fragments arranged by size: 

                 308     281     238     161      32
 
BspHI T'CATG_A

Cuts at:      0     299     560     782    1020 
   Size:        299     261     222     238

  Fragments arranged by size: 

                 299     261     238     222
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     754     904    1020 
   Size:        754     150     116

  Fragments arranged by size: 

                 754     150     116
 
BsrBI CCG'CTC

Cuts at:      0     842    1020 
   Size:        842     178
 
BsrDI GCAATG_nn'

Cuts at:      0     720    1020 
   Size:        720     300
 
BsrFI r'CCGG_y

Cuts at:      0     517     653    1020 
   Size:        517     136     367

  Fragments arranged by size: 

                 517     367     136
 
BssSI C'ACGA_G

Cuts at:      0     138     383     673    1020 
   Size:        138     245     290     347

  Fragments arranged by size: 

                 347     290     245     138
 
BstEII G'GTnAC_C

Cuts at:      0     430    1020 
   Size:        430     590
 
BstYI r'GATC_y

Cuts at:      0     170    1010    1020 
   Size:        170     840      10

  Fragments arranged by size: 

                 840     170      10
 
BtsI GCAGTG_nn'

Cuts at:      0     148    1020 
   Size:        148     872
 
DraIII CAC_nnn'GTG

Cuts at:      0     494    1020 
   Size:        494     526
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     940    1020 
   Size:        940      80
 
EaeI y'GGCC_r

Cuts at:      0     164     519     669    1020 
   Size:        164     355     150     351

  Fragments arranged by size: 

                 355     351     164     150
 
EcoICRI GAG'CTC

Cuts at:      0     780    1020 
   Size:        780     240
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     503     572     602    1020 
   Size:        183     320      69      30     418

  Fragments arranged by size: 

                 418     320     183      69      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     989    1020 
   Size:        989      31
 
FspI TGC'GCA

Cuts at:      0     136    1020 
   Size:        136     884
 
FspAI rTGC'GCAy

Cuts at:      0     136    1020 
   Size:        136     884
 
HaeII r_GCGC'y

Cuts at:      0     849    1020 
   Size:        849     171
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     581     613    1020 
   Size:        581      32     407

  Fragments arranged by size: 

                 581     407      32
 
KasI G'GCGC_C

Cuts at:      0     845    1020 
   Size:        845     175
 
MscI TGG'CCA

Cuts at:      0     166     671    1020 
   Size:        166     505     349

  Fragments arranged by size: 

                 505     349     166
 
MslI CAynn'nnrTG

Cuts at:      0     976    1020 
   Size:        976      44
 
MspA1I CmG'CkG

Cuts at:      0     947    1020 
   Size:        947      73
 
NaeI GCC'GGC

Cuts at:      0     655    1020 
   Size:        655     365
 
NarI GG'CG_CC

Cuts at:      0     846    1020 
   Size:        846     174
 
NdeI CA'TA_TG

Cuts at:      0     312    1020 
   Size:        312     708
 
NgoMIV G'CCGG_C

Cuts at:      0     653    1020 
   Size:        653     367
 
NsiI A_TGCA'T

Cuts at:      0     720    1020 
   Size:        720     300
 
NspI r_CATG'y

Cuts at:      0      74     651    1020 
   Size:         74     577     369

  Fragments arranged by size: 

                 577     369      74
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     185     629    1020 
   Size:        185     444     391

  Fragments arranged by size: 

                 444     391     185
 
PfoI T'CCnGG_A

Cuts at:      0     452    1020 
   Size:        452     568
 
PmlI CAC'GTG

Cuts at:      0     494    1020 
   Size:        494     526
 
PpuMI rG'GwC_Cy

Cuts at:      0     989    1020 
   Size:        989      31
 
PstI C_TGCA'G

Cuts at:      0     751    1020 
   Size:        751     269
 
PvuII CAG'CTG

Cuts at:      0     947    1020 
   Size:        947      73
 
SacI G_AGCT'C

Cuts at:      0     782    1020 
   Size:        782     238
 
SbfI CC_TGCA'GG

Cuts at:      0     751    1020 
   Size:        751     269
 
SfcI C'TryA_G

Cuts at:      0     747    1020 
   Size:        747     273
 
SfoI GGC'GCC

Cuts at:      0     847    1020 
   Size:        847     173
 
SmlI C'TyrA_G

Cuts at:      0      40    1006    1020 
   Size:         40     966      14

  Fragments arranged by size: 

                 966      40      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      57      89    1020 
   Size:         57      32     931

  Fragments arranged by size: 

                 931      57      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     987    1020 
   Size:        987      33
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AhdI     BanI    BanII     BbeI
    BfrBI     BglI    BglII Bme1580I     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstEII    BstYI     BtsI   DraIII     DrdI
     EaeI  EcoICRI  Eco57MI EcoO109I     FspI    FspAI    HaeII    Hin4I
     KasI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NsiI     NspI    PflMI     PfoI     PmlI    PpuMI     PstI    PvuII
     SacI     SbfI     SfcI     SfoI     SmlI     TstI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI     BlpI    BmgBI     BmtI   Bpu10I    BsiWI     BsmI    BspEI
    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     EagI     EarI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NcoI     NheI     NotI     NruI     PacI
     PasI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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