Restriction map for pF1KB3424
 (Circular) (Six-base) MAPSORT of: pF1KB3424.seq  Check: 4391  from: 1  to: 4433



pF1KB3424  4433 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    478     524    1471     478 
   Size:         46     947    3440

  Fragments arranged by size: 

                3440     947      46
 
Acc65I G'GTAC_C

Cuts at:   1449    1449 
   Size:       4433
 
AclI AA'CG_TT

Cuts at:   4273    4273 
   Size:       4433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    316     950    2258    2690    3043    3705     316 
   Size:        634    1308     432     353     662    1044

  Fragments arranged by size: 

                1308    1044     662     634     432     353
 
AflII C'TTAA_G

Cuts at:   1326    1326 
   Size:       4433
 
AflIII A'CryG_T

Cuts at:   1785    3585    3866    1785 
   Size:       1800     281    2352

  Fragments arranged by size: 

                2352    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    241    1312    1738    3176     241 
   Size:       1071     426    1438    1498

  Fragments arranged by size: 

                1498    1438    1071     426
 
ApaI G_GGCC'C

Cuts at:    899     899 
   Size:       4433
 
ApaLI G'TGCA_C

Cuts at:   3271    3271 
   Size:       4433
 
ApoI r'AATT_y

Cuts at:    337     686    1019    1437    3786    4042     337 
   Size:        349     333     418    2349     256     728

  Fragments arranged by size: 

                2349     728     418     349     333     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4433
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4433
 
AvaI C'yCGr_G

Cuts at:   1453    1453 
   Size:       4433
 
AvrII C'CTAG_G

Cuts at:    885     885 
   Size:       4433
 
BamHI G'GATC_C

Cuts at:    609    1119    1458     609 
   Size:        510     339    3584

  Fragments arranged by size: 

                3584     510     339
 
BanI G'GyrC_C

Cuts at:   1405    1449    1863    2111    2146    1405 
   Size:         44     414     248      35    3692

  Fragments arranged by size: 

                3692     414     248      44      35
 
BanII G_rGCy'C

Cuts at:    493     899    1447    2477     493 
   Size:        406     548    1030    2449

  Fragments arranged by size: 

                2449    1030     548     406
 
BbeI G_GCGC'C

Cuts at:   1867    2115    1867 
   Size:        248    4185
 
BbsI GAAGACnn'nnnn_

Cuts at:   1364    1364 
   Size:       4433
 
BbvCI CC'TCA_GC

Cuts at:    837     837 
   Size:       4433
 
BciVI GTATCCnnnnn_n'

Cuts at:   2331    3387    4433    2331 
   Size:       1056    1046    2331

  Fragments arranged by size: 

                2331    1056    1046
 
BclI T'GATC_A

Cuts at:   1256    1953    1256 
   Size:        697    3736
 
BglII A'GATC_T

Cuts at:   1948    3985    1948 
   Size:       2037    2396
 
BlpI GC'TnA_GC

Cuts at:   1545    1545 
   Size:       4433
 
Bme1580I G_kGCm'C

Cuts at:    899    2058    2151    3275     899 
   Size:       1159      93    1124    2057

  Fragments arranged by size: 

                2057    1159    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1645    1726    2061    4205    1645 
   Size:         81     335    2144    1873

  Fragments arranged by size: 

                2144    1873     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2800    2800 
   Size:       4433
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    161     161 
   Size:       4433
 
Bpu10I CC'TnA_GC

Cuts at:    837     837 
   Size:       4433
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    870    1609    2488    3202    3500     870 
   Size:        739     879     714     298    1803

  Fragments arranged by size: 

                1803     879     739     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    646    4137     646 
   Size:       3491     942
 
BsaAI yAC'GTr

Cuts at:   1786    1786 
   Size:       4433
 
BsaBI GATnn'nnATC

Cuts at:      6     461    1947    3984       6 
   Size:        455    1486    2037     455

  Fragments arranged by size: 

                2037    1486     455     455
 
BsaHI Gr'CG_yC

Cuts at:    971    1864    2112    2814    4310     971 
   Size:        893     248     702    1496    1094

  Fragments arranged by size: 

                1496    1094     893     702     248
 
BsaWI w'CCGG_w

Cuts at:   1622    2143    2821    3232    3379    3653    1622 
   Size:        521     678     411     147     274    2402

  Fragments arranged by size: 

                2402     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    400     400 
   Size:       4433
 
BseYI C'CCAG_C

Cuts at:   1858    2616    3281    3689    1858 
   Size:        758     665     408    2602

  Fragments arranged by size: 

                2602     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104    1975    2021    3251     104 
   Size:       1871      46    1230    1286

  Fragments arranged by size: 

                1871    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1447    2225    2415    3275    1447 
   Size:        778     190     860    2605

  Fragments arranged by size: 

                2605     860     778     190
 
BsiWI C'GTAC_G

Cuts at:    906     906 
   Size:       4433
 
BsmI GAATG_Cn'

Cuts at:    509     509 
   Size:       4433
 
Bsp1286I G_dGCh'C

Cuts at:    493     899    1447    2058    2151    2225    2415    2477    3275 
   Size:        406     548     611      93      74     190      62     798

Cuts at:   3275     493 
   Size:       1651

  Fragments arranged by size: 

                1651     798     611     548     406     190      93      74
                  62
 
BspHI T'CATG_A

Cuts at:   2865    2865 
   Size:       4433
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1483    1697    1999    2380    1483 
   Size:        214     302     381    3536

  Fragments arranged by size: 

                3536     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2725    2779    4263    4428      50 
   Size:       2675      54    1484     165      55

  Fragments arranged by size: 

                2675    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2345    2345 
   Size:       4433
 
BsrFI r'CCGG_y

Cuts at:    281    2431    2612     281 
   Size:       2150     181    2102

  Fragments arranged by size: 

                2150    2102     181
 
BsrGI T'GTAC_A

Cuts at:   1303    1303 
   Size:       4433
 
BssSI C'ACGA_G

Cuts at:   2704    3412    2704 
   Size:        708    3725
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1810    1810 
   Size:       4433
 
BstBI TT'CG_AA

Cuts at:   2794    2794 
   Size:       4433
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1057    1057 
   Size:       4433
 
BstYI r'GATC_y

Cuts at:      1     531     609    1119    1458    1925    1948    2283    2529 
   Size:        530      78     510     339     467      23     335     246

Cuts at:   2529    2835    2847    2933    3985       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                1052     530     510     467     449     339     335     306
                 246      86      78      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    764    1412     764 
   Size:        648    3785
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4433
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2383    2564    2727    2383 
   Size:        181     163    4089

  Fragments arranged by size: 

                4089     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1676    1676 
   Size:       4433
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    185     220     987    1022     185 
   Size:         35     767      35    3596

  Fragments arranged by size: 

                3596     767      35      35
 
DraI TTT'AAA

Cuts at:   1432    1432 
   Size:       4433
 
DraIII CAC_nnn'GTG

Cuts at:    278     573     278 
   Size:        295    4138
 
DrdI GACnn_nn'nnGTC

Cuts at:    426    2139    3483     426 
   Size:       1713    1344    1376

  Fragments arranged by size: 

                1713    1376    1344
 
EaeI y'GGCC_r

Cuts at:   1094    2018    2192    2583    2610    3955    1094 
   Size:        924     174     391      27    1345    1572

  Fragments arranged by size: 

                1572    1345     924     391     174      27
 
EagI C'GGCC_G

Cuts at:   2018    2018 
   Size:       4433
 
EarI CTCTTCn'nnn_

Cuts at:    440     737    2456    2666     440 
   Size:        297    1719     210    2207

  Fragments arranged by size: 

                2207    1719     297     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2641    3383    3529    4243    2641 
   Size:        742     146     714    2831

  Fragments arranged by size: 

                2831     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1445    1445 
   Size:       4433
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    161     316     950    2258    2690    3043    3705     161 
   Size:        155     634    1308     432     353     662     889

  Fragments arranged by size: 

                1308     889     662     634     432     353     155
 
EcoNI CCTnn'n_nnAGG

Cuts at:    794     794 
   Size:       4433
 
EcoO109I rG'GnC_Cy

Cuts at:    419     642    1572     419 
   Size:        223     930    3280

  Fragments arranged by size: 

                3280     930     223
 
EcoRI G'AATT_C

Cuts at:    337    1437     337 
   Size:       1100    3333
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    337    1215    2452     337 
   Size:        878    1237    2318

  Fragments arranged by size: 

                2318    1237     878
 
FspI TGC'GCA

Cuts at:   2214    2214 
   Size:       4433
 
HaeII r_GCGC'y

Cuts at:   1867    2115    3345    4116    1867 
   Size:        248    1230     771    2184

  Fragments arranged by size: 

                2184    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    114     146     379     411    1261    1293    2275    2305    2307 
   Size:         32     233      32     850      32     982      30       2

Cuts at:   2307    2337     114 
   Size:         30    2210

  Fragments arranged by size: 

                2210     982     850     233      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1472    1472 
   Size:       4433
 
KasI G'GCGC_C

Cuts at:   1863    2111    1863 
   Size:        248    4185
 
KpnI G_GTAC'C

Cuts at:   1453    1453 
   Size:       4433
 
MluI A'CGCG_T

Cuts at:   3866    3866 
   Size:       4433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    656    1016    1034    3192    3376     656 
   Size:        360      18    2158     184    1713

  Fragments arranged by size: 

                2158    1713     360     184      18
 
MscI TGG'CCA

Cuts at:   1096    2194    1096 
   Size:       1098    3335
 
MslI CAynn'nnrTG

Cuts at:    625    2422    2549     625 
   Size:       1797     127    2509

  Fragments arranged by size: 

                2509    1797     127
 
MspA1I CmG'CkG

Cuts at:   1544    1858    2218    3002    3247    1544 
   Size:        314     360     784     245    2730

  Fragments arranged by size: 

                2730     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   2614    2614 
   Size:       4433
 
NarI GG'CG_CC

Cuts at:   1864    2112    1864 
   Size:        248    4185
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4433
 
NgoMIV G'CCGG_C

Cuts at:   2612    2612 
   Size:       4433
 
NspI r_CATG'y

Cuts at:    580     940    1486    1986    3589     580 
   Size:        360     546     500    1603    1424

  Fragments arranged by size: 

                1603    1424     546     500     360
 
PciI A'CATG_T

Cuts at:   3585    3585 
   Size:       4433
 
PfoI T'CCnGG_A

Cuts at:    138     138 
   Size:       4433
 
PmeI GTTT'AAAC

Cuts at:   1432    1432 
   Size:       4433
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2879    2911    2879 
   Size:         32    4401
 
PpuMI rG'GwC_Cy

Cuts at:    419     419 
   Size:       4433
 
PsiI TTA'TAA

Cuts at:   1391    1391 
   Size:       4433
 
PspOMI G'GGCC_C

Cuts at:    895     895 
   Size:       4433
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    176     208     176 
   Size:         32    4401
 
PstI C_TGCA'G

Cuts at:   1480    2165    1480 
   Size:        685    3748
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4433
 
PvuII CAG'CTG

Cuts at:   1858    2218    1858 
   Size:        360    4073
 
RsrII CG'GwC_CG

Cuts at:   2628    2628 
   Size:       4433
 
SacI G_AGCT'C

Cuts at:   1447    1447 
   Size:       4433
 
SalI G'TCGA_C

Cuts at:   1470    1470 
   Size:       4433
 
SanDI GG'GwC_CC

Cuts at:    419     419 
   Size:       4433
 
SapI GCTCTTCn'nnn_

Cuts at:   2456    2666    2456 
   Size:        210    4223
 
SbfI CC_TGCA'GG

Cuts at:   1480    1480 
   Size:       4433
 
ScaI AGT'ACT

Cuts at:   3950    3950 
   Size:       4433
 
SfcI C'TryA_G

Cuts at:     33    1476    2161    3129    3320      33 
   Size:       1443     685     968     191    1146

  Fragments arranged by size: 

                1443    1146     968     685     191
 
SfoI GGC'GCC

Cuts at:   1865    2113    1865 
   Size:        248    4185
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4433
 
SmaI CCC'GGG

Cuts at:   1455    1455 
   Size:       4433
 
SmlI C'TyrA_G

Cuts at:    849    1326    1588    2503    3217    3479     849 
   Size:        477     262     915     714     262    1803

  Fragments arranged by size: 

                1803     915     714     477     262     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4433
 
SphI G_CATG'C

Cuts at:    580    1486    1986     580 
   Size:        906     500    3027

  Fragments arranged by size: 

                3027     906     500
 
SspI AAT'ATT

Cuts at:    736     736 
   Size:       4433
 
StyI C'CwwG_G

Cuts at:    107     440     885    1567    1921     107 
   Size:        333     445     682     354    2619

  Fragments arranged by size: 

                2619     682     445     354     333
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    543     739    2016    2149    2608    2817     543 
   Size:        196    1277     133     459     209    2159

  Fragments arranged by size: 

                2159    1277     459     209     196     133
 
TatI w'GTAC_w

Cuts at:   1303    3948    1303 
   Size:       2645    1788
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3950    3982    3950 
   Size:         32    4401
 
Tth111I GACn'n_nGTC

Cuts at:   2230    2230 
   Size:       4433
 
XbaI T'CTAG_A

Cuts at:   1464    1464 
   Size:       4433
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1928    1928 
   Size:       4433
 
XmaI C'CCGG_G

Cuts at:   1453    1453 
   Size:       4433
 
XmnI GAAnn'nnTTC

Cuts at:    872    1382     872 
   Size:        510    3923
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PfoI     PmeI     PpiI    PpuMI     PsiI   PspOMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AleI     AlfI     AloI
     AscI     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI    BsaXI
     BsgI    BsmBI    BspEI   BssHII   BstEII  BstZ17I     ClaI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PshAI    PspXI
    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI
     XhoI     ZraI
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