Restriction map for
pF1KB3403
(Circular) (Six-base) MAPSORT of: pF1KB3403.seq Check: 4558 from: 1 to: 3878
pF1KB3403 3878 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 916 916
Size: 3878
Acc65I G'GTAC_C
Cuts at: 894 894
Size: 3878
AclI AA'CG_TT
Cuts at: 3718 3718
Size: 3878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 631 859 1703 2135 2882 3150 387
Size: 244 228 844 432 747 268 1115
Fragments arranged by size:
1115 844 747 432 268 244 228
AflIII A'CryG_T
Cuts at: 1230 2334 3311 1230
Size: 1104 977 1797
Fragments arranged by size:
1797 1104 977
AgeI A'CCGG_T
Cuts at: 2266 2266
Size: 3878
AlwNI CAG_nnn'CTG
Cuts at: 1183 2750 1183
Size: 1567 2311
ApaLI G'TGCA_C
Cuts at: 2648 2648
Size: 3878
ApoI r'AATT_y
Cuts at: 882 3231 3487 882
Size: 2349 256 1273
Fragments arranged by size:
2349 1273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 898 898
Size: 3878
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 894 1308 1556 1591 894
Size: 414 248 35 3181
Fragments arranged by size:
3181 414 248 35
BanII G_rGCy'C
Cuts at: 115 892 1922 115
Size: 777 1030 2071
Fragments arranged by size:
2071 1030 777
BbeI G_GCGC'C
Cuts at: 1312 1560 1312
Size: 248 3630
BbsI GAAGACnn'nnnn_
Cuts at: 783 783
Size: 3878
BciVI GTATCCnnnnn_n'
Cuts at: 1776 2537 3878 1776
Size: 761 1341 1776
Fragments arranged by size:
1776 1341 761
BclI T'GATC_A
Cuts at: 1398 1398
Size: 3878
BfrBI ATG'CAT
Cuts at: 472 472
Size: 3878
BglII A'GATC_T
Cuts at: 406 1393 3430 406
Size: 987 2037 854
Fragments arranged by size:
2037 987 854
BlpI GC'TnA_GC
Cuts at: 990 990
Size: 3878
Bme1580I G_kGCm'C
Cuts at: 1503 1596 2652 1503
Size: 93 1056 2729
Fragments arranged by size:
2729 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 134 1090 1171 1506 3650 134
Size: 956 81 335 2144 362
Fragments arranged by size:
2144 956 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 3878
Bpu10I CC'TnA_GC
Cuts at: 827 827
Size: 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1933 2425 2723 1054
Size: 879 492 298 2209
Fragments arranged by size:
2209 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 1231 1231
Size: 3878
BsaBI GATnn'nnATC
Cuts at: 6 1392 3429 6
Size: 1386 2037 455
Fragments arranged by size:
2037 1386 455
BsaHI Gr'CG_yC
Cuts at: 1309 1557 2259 3755 1309
Size: 248 702 1496 1432
Fragments arranged by size:
1496 1432 702 248
BsaWI w'CCGG_w
Cuts at: 1067 1588 2266 2540 2687 3098 1067
Size: 521 678 274 147 411 1847
Fragments arranged by size:
1847 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 138 168 138
Size: 30 3848
BseYI C'CCAG_C
Cuts at: 752 1303 2061 2638 3134 752
Size: 551 758 577 496 1496
Fragments arranged by size:
1496 758 577 551 496
BsiEI CG_ry'CG
Cuts at: 104 1420 1466 2674 104
Size: 1316 46 1208 1308
Fragments arranged by size:
1316 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 892 1670 1860 2652 115
Size: 777 778 190 792 1341
Fragments arranged by size:
1341 792 778 777 190
Bsp1286I G_dGCh'C
Cuts at: 115 892 1503 1596 1670 1860 1922 2652 115
Size: 777 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 777 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 673 928 1142 1444 1825 334
Size: 339 255 214 302 381 2387
Fragments arranged by size:
2387 381 339 302 255 214
BsrBI CCG'CTC
Cuts at: 50 2170 2224 3708 3873 50
Size: 2120 54 1484 165 55
Fragments arranged by size:
2120 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 1876 2057 2266 1876
Size: 181 209 3488
Fragments arranged by size:
3488 209 181
BssSI C'ACGA_G
Cuts at: 2149 2507 2149
Size: 358 3520
BstAPI GCAn_nnn'nTGC
Cuts at: 1255 1255
Size: 3878
BstBI TT'CG_AA
Cuts at: 2239 2239
Size: 3878
BstYI r'GATC_y
Cuts at: 1 406 903 1370 1393 1728 1974 2986 3072
Size: 405 497 467 23 335 246 1012 86
Cuts at: 3072 3084 3430 1
Size: 12 346 449
Fragments arranged by size:
1012 497 467 449 405 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 1121 1121
Size: 3878
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 455 1463 1637 2028 2055 3400 455
Size: 1008 174 391 27 1345 933
Fragments arranged by size:
1345 1008 933 391 174 27
EagI C'GGCC_G
Cuts at: 1463 1463
Size: 3878
EarI CTCTTCn'nnn_
Cuts at: 159 1901 2111 159
Size: 1742 210 1926
Fragments arranged by size:
1926 1742 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2086 2396 2542 3688 2086
Size: 310 146 1146 2276
Fragments arranged by size:
2276 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 890 113
Size: 777 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 631 859 1703 2135 2882 3150 387
Size: 244 228 844 432 747 268 1115
Fragments arranged by size:
1115 844 747 432 268 244 228
EcoO109I rG'GnC_Cy
Cuts at: 1017 1017
Size: 3878
EcoRI G'AATT_C
Cuts at: 882 882
Size: 3878
EcoRV GAT'ATC
Cuts at: 675 675
Size: 3878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3878
FspI TGC'GCA
Cuts at: 548 1659 548
Size: 1111 2767
HaeII r_GCGC'y
Cuts at: 1312 1560 2582 3561 1312
Size: 248 1022 979 1629
Fragments arranged by size:
1629 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1750 1752 1782 1720
Size: 30 2 30 3816
Fragments arranged by size:
3816 30 30 2
HincII GTy'rAC
Cuts at: 917 917
Size: 3878
HindIII A'AGCT_T
Cuts at: 392 643 731 392
Size: 251 88 3539
Fragments arranged by size:
3539 251 88
KasI G'GCGC_C
Cuts at: 1308 1556 1308
Size: 248 3630
KpnI G_GTAC'C
Cuts at: 898 898
Size: 3878
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 309 320 422 2549 2733 309
Size: 11 102 2127 184 1454
Fragments arranged by size:
2127 1454 184 102 11
MscI TGG'CCA
Cuts at: 457 1639 457
Size: 1182 2696
MslI CAynn'nnrTG
Cuts at: 463 1867 1994 463
Size: 1404 127 2347
Fragments arranged by size:
2347 1404 127
MspA1I CmG'CkG
Cuts at: 752 989 1303 1663 2676 2921 752
Size: 237 314 360 1013 245 1709
Fragments arranged by size:
1709 1013 360 314 245 237
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 3878
NarI GG'CG_CC
Cuts at: 1309 1557 1309
Size: 248 3630
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 3878
NsiI A_TGCA'T
Cuts at: 474 474
Size: 3878
NspI r_CATG'y
Cuts at: 328 472 931 1431 2338 328
Size: 144 459 500 907 1868
Fragments arranged by size:
1868 907 500 459 144
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 731 763 3017 3049 731
Size: 32 2254 32 1560
Fragments arranged by size:
2254 1560 32 32
PstI C_TGCA'G
Cuts at: 511 925 1610 511
Size: 414 685 2779
Fragments arranged by size:
2779 685 414
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 752 1303 1663 752
Size: 551 360 2967
Fragments arranged by size:
2967 551 360
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 115 892 115
Size: 777 3101
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
SbfI CC_TGCA'GG
Cuts at: 925 925
Size: 3878
ScaI AGT'ACT
Cuts at: 3395 3395
Size: 3878
SfcI C'TryA_G
Cuts at: 33 483 507 921 1606 2599 2790 33
Size: 450 24 414 685 993 191 1121
Fragments arranged by size:
1121 993 685 450 414 191 24
SfoI GGC'GCC
Cuts at: 1310 1558 1310
Size: 248 3630
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SmaI CCC'GGG
Cuts at: 900 900
Size: 3878
SmlI C'TyrA_G
Cuts at: 1033 1948 2440 2702 1033
Size: 915 492 262 2209
Fragments arranged by size:
2209 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 472 931 1431 472
Size: 459 500 2919
Fragments arranged by size:
2919 500 459
StyI C'CwwG_G
Cuts at: 1012 1366 1012
Size: 354 3524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1461 1594 2053 2262 1461
Size: 133 459 209 3077
Fragments arranged by size:
3077 459 209 133
TatI w'GTAC_w
Cuts at: 211 708 3393 211
Size: 497 2685 696
Fragments arranged by size:
2685 696 497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 1675 1675
Size: 3878
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 1373
Size: 3878
XmaI C'CCGG_G
Cuts at: 898 898
Size: 3878
XmnI GAAnn'nnTTC
Cuts at: 788 788
Size: 3878
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI