Restriction map for pF1KB3385
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwNI       
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseRI       BsgI        BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    EcoNI       
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HpaI        HphI        KasI        KspI        
MaeI        MamI        MfeI        MluI        MseI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        282  GGTACC               G^GTACC
AccII         310  CGCG                 CG^CG
AccIII        200  TCCGGA               T^CCGGA
Alw44I        302  GTGCAC               G^TGCAC
ApaI          323  GGGCCC               GGGCC^C
ApaLI         302  GTGCAC               G^TGCAC
Asp718I       282  GGTACC               G^GTACC
AspHI         302  GTGCAC               G[AT]GC[AT]^C
BalI          104  TGGCCA               TGG^CCA
BanI          282  GGTACC               G^G[CT][GA]CC
BbvI          329  GCAGC                GCAGC(8/12)
BseAI         200  TCCGGA               T^CCGGA
BsiHKAI       302  GTGCAC               G[AT]GC[AT]^C
BspEI         200  TCCGGA               T^CCGGA
BsrBI         501  CCGCTC               CCGCTC(-3/-3)
BstUI         310  CGCG                 CG^CG
BstYI         356  AGATCC               [GA]^GATC[CT]
DraII         461  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          239  CCGTGG               C^C[GA][CT]GG
EarI          144  GAAGAG               CTCTTC(1/4)
EcoO109I      461  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRV          64  GATATC               GAT^ATC
EcoT14I       506  CCAAGG               C^C[AT][AT]GG
HindIII       211  AAGCTT               A^AGCTT
KpnI          282  GGTACC               GGTAC^C
Ksp632I       144  GAAGAG               CTCTTC(1/4)
MaeII         497  ACGT                 A^CGT
MaeIII        313  GTGAC                ^GT[ACGT]AC
MflI          356  AGATCC               [GA]^GATC[CT]
MluNI         104  TGGCCA               TGG^CCA
MroI          200  TCCGGA               T^CCGGA
MscI          104  TGGCCA               TGG^CCA
MspA1I        373  CAGCTG               C[AC]G^C[GT]G
MvnI          310  CGCG                 CG^CG
PvuII         373  CAGCTG               CAG^CTG
SexAI         131  ACCAGGT              A^CC[AT]GGT
SfaNI         335  GCATC                GCATC(5/9)
StyI          506  CCAAGG               C^C[AT][AT]GG
TseI          329  GCAGC                G^C[AT]GC
Tsp45I        313  GTGAC                ^GT[GC]AC
XhoII         356  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          283  GTAC                 GT^AC
              444  GTAC                
AluI          212  AGCT                 AG^CT
              374  AGCT                
AlwI          198  GATCC                GGATC(4/5)
              357  GATCC               
BanII          49  GAGCCC               G[GA]GC[CT]^C
              323  GGGCCC              
BsaWI         200  TCCGGA               [AT]^CCGG[AT]
              418  TCCGGT              
BsiEI         172  CGGCCG               CG[GA][CT]^CG
              236  CGGCCG              
BsmFI         250  GGGAC                GGGAC(10/14)
              183  GTCCC               
BsrFI         170  ACCGGC               [GA]^CCGG[CT]
              446  ACCGGC              
CfoI           42  GCGC                 GCG^C
              309  GCGC                
Cfr10I        170  ACCGGC               [GA]^CCGG[CT]
              446  ACCGGC              
DpnII         198  GATC                 ^GATC
              357  GATC                
EagI          172  CGGCCG               C^GGCCG
              236  CGGCCG              
EclXI         172  CGGCCG               C^GGCCG
              236  CGGCCG              
Eco52I        172  CGGCCG               C^GGCCG
              236  CGGCCG              
HhaI           42  GCGC                 GCG^C
              309  GCGC                
HinP1I         42  GCGC                 G^CGC
              309  GCGC                
HinfI         159  GAGTC                G^A[ACGT]TC
              385  GACTC               
MboI          198  GATC                 ^GATC
              357  GATC                
NdeII         198  GATC                 ^GATC
              357  GATC                
PleI          385  GACTC                GAGTC(4/5)
              159  GAGTC               
RsaI          283  GTAC                 GT^AC
              444  GTAC                
Sau3AI        198  GATC                 ^GATC
              357  GATC                
TaqI          368  TCGA                 T^CGA
              521  TCGA                
TspRI         305  CACTG                CA[GC]TG[ACGT][ACGT]^
              405  CACTG               
TthHB8I       368  TCGA                 T^CGA
              521  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AocI        AvaII       BcnI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstF5I      BstNI       Bsu36I      Cac8I       Cfr13I      
DdeI        EaeI        Eco81I      EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
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