Restriction map for pF1KB3372
 (Linear) (Six-base) MAPSORT of: KIBB3372.seq  Check: 1516  from: 1  to: 1335



KIBB3372.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AccI GT'mk_AC

Cuts at:      0     190     677    1335 
   Size:        190     487     658

  Fragments arranged by size: 

                 658     487     190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     891    1335 
   Size:        172     719     444

  Fragments arranged by size: 

                 719     444     172
 
AflII C'TTAA_G

Cuts at:      0    1056    1335 
   Size:       1056     279
 
AflIII A'CryG_T

Cuts at:      0     446    1335 
   Size:        446     889
 
AhdI GACnn_n'nnGTC

Cuts at:      0     186    1335 
   Size:        186    1149
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     599    1335 
   Size:        599     736
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465    1335 
   Size:        465     870
 
ApoI r'AATT_y

Cuts at:      0      47    1078    1335 
   Size:         47    1031     257

  Fragments arranged by size: 

                1031     257      47
 
AvaI C'yCGr_G

Cuts at:      0     516    1335 
   Size:        516     819
 
BanI G'GyrC_C

Cuts at:      0     232    1144    1335 
   Size:        232     912     191

  Fragments arranged by size: 

                 912     232     191
 
BanII G_rGCy'C

Cuts at:      0     356     905    1139    1335 
   Size:        356     549     234     196

  Fragments arranged by size: 

                 549     356     234     196
 
BbeI G_GCGC'C

Cuts at:      0     236    1335 
   Size:        236    1099
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      70     860    1335 
   Size:         70     790     475

  Fragments arranged by size: 

                 790     475      70
 
BglI GCCn_nnn'nGGC

Cuts at:      0     169     557    1335 
   Size:        169     388     778

  Fragments arranged by size: 

                 778     388     169
 
BmgBI CAC'GTC

Cuts at:      0     888    1335 
   Size:        888     447
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1228    1335 
   Size:       1228     107
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     531    1335 
   Size:        531     804
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1012    1169    1244    1335 
   Size:        295     717     157      75      91

  Fragments arranged by size: 

                 717     295     157      91      75
 
Bpu10I CC'TnA_GC

Cuts at:      0     840     917    1133    1335 
   Size:        840      77     216     202

  Fragments arranged by size: 

                 840     216     202      77
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838    1335 
   Size:        838     497
 
BsaAI yAC'GTr

Cuts at:      0     449    1335 
   Size:        449     886
 
BsaBI GATnn'nnATC

Cuts at:      0     145    1335 
   Size:        145    1190
 
BsaHI Gr'CG_yC

Cuts at:      0     233     985    1335 
   Size:        233     752     350

  Fragments arranged by size: 

                 752     350     233
 
BsaWI w'CCGG_w

Cuts at:      0     437    1335 
   Size:        437     898
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     700     730    1335 
   Size:        700      30     605

  Fragments arranged by size: 

                 700     605      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     947    1335 
   Size:        947     388
 
BseYI C'CCAG_C

Cuts at:      0     546    1335 
   Size:        546     789
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     114     688     936    1335 
   Size:         73      41     574     248     399

  Fragments arranged by size: 

                 574     399     248      73      41
 
BsiEI CG_ry'CG

Cuts at:      0     123    1335 
   Size:        123    1212
 
BsiHKAI G_wGCw'C

Cuts at:      0     356    1139    1322    1335 
   Size:        356     783     183      13

  Fragments arranged by size: 

                 783     356     183      13
 
BsmI GAATG_Cn'

Cuts at:      0     793    1335 
   Size:        793     542
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     535     720    1335 
   Size:        535     185     615

  Fragments arranged by size: 

                 615     535     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     356     905    1139    1322    1335 
   Size:        356     549     234     183      13

  Fragments arranged by size: 

                 549     356     234     183      13
 
BspHI T'CATG_A

Cuts at:      0      74    1335 
   Size:         74    1261
 
BsrBI CCG'CTC

Cuts at:      0     523     654    1335 
   Size:        523     131     681

  Fragments arranged by size: 

                 681     523     131
 
BsrFI r'CCGG_y

Cuts at:      0     346    1335 
   Size:        346     989
 
BssSI C'ACGA_G

Cuts at:      0     205    1335 
   Size:        205    1130
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     693    1335 
   Size:        693     642
 
BstYI r'GATC_y

Cuts at:      0     500    1019    1335 
   Size:        500     519     316

  Fragments arranged by size: 

                 519     500     316
 
BtgI C'CryG_G

Cuts at:      0     227     389     534     813    1205    1335 
   Size:        227     162     145     279     392     130

  Fragments arranged by size: 

                 392     279     227     162     145     130
 
BtsI GCAGTG_nn'

Cuts at:      0     321     463     723     962    1335 
   Size:        321     142     260     239     373

  Fragments arranged by size: 

                 373     321     260     239     142
 
EaeI y'GGCC_r

Cuts at:      0     120     161    1335 
   Size:        120      41    1174

  Fragments arranged by size: 

                1174     120      41
 
EagI C'GGCC_G

Cuts at:      0     120    1335 
   Size:        120    1215
 
EarI CTCTTCn'nnn_

Cuts at:      0     389    1335 
   Size:        389     946
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      52    1012    1151    1335 
   Size:         52     960     139     184

  Fragments arranged by size: 

                 960     184     139      52
 
EcoICRI GAG'CTC

Cuts at:      0     354    1137    1335 
   Size:        354     783     198

  Fragments arranged by size: 

                 783     354     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     295     891    1012    1169    1244    1335 
   Size:        172     123     596     121     157      75      91

  Fragments arranged by size: 

                 596     172     157     123     121      91      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     856    1335 
   Size:        856     479
 
EcoO109I rG'GnC_Cy

Cuts at:      0     156     270     288    1335 
   Size:        156     114      18    1047

  Fragments arranged by size: 

                1047     156     114      18
 
EcoRI G'AATT_C

Cuts at:      0    1078    1335 
   Size:       1078     257
 
EcoRV GAT'ATC

Cuts at:      0    1159    1335 
   Size:       1159     176
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1098    1335 
   Size:       1098     237
 
HaeII r_GCGC'y

Cuts at:      0     112     236    1099    1279    1335 
   Size:        112     124     863     180      56

  Fragments arranged by size: 

                 863     180     124     112      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     599     631     700     732     970    1002 
   Size:        172      32     395      32      69      32     238      32

Cuts at:   1002    1335 
   Size:        333

  Fragments arranged by size: 

                 395     333     238     172      69      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     191    1335 
   Size:        191    1144
 
HindIII A'AGCT_T

Cuts at:      0    1053    1335 
   Size:       1053     282
 
KasI G'GCGC_C

Cuts at:      0     232    1335 
   Size:        232    1103
 
MscI TGG'CCA

Cuts at:      0     163    1335 
   Size:        163    1172
 
MslI CAynn'nnrTG

Cuts at:      0    1234    1335 
   Size:       1234     101
 
MspA1I CmG'CkG

Cuts at:      0     403    1009    1335 
   Size:        403     606     326

  Fragments arranged by size: 

                 606     403     326
 
NaeI GCC'GGC

Cuts at:      0     348    1335 
   Size:        348     987
 
NarI GG'CG_CC

Cuts at:      0     233    1335 
   Size:        233    1102
 
NcoI C'CATG_G

Cuts at:      0    1205    1335 
   Size:       1205     130
 
NgoMIV G'CCGG_C

Cuts at:      0     346    1335 
   Size:        346     989
 
PasI CC'CwG_GG

Cuts at:      0     660    1335 
   Size:        660     675
 
PfoI T'CCnGG_A

Cuts at:      0    1185    1335 
   Size:       1185     150
 
PmlI CAC'GTG

Cuts at:      0     449    1335 
   Size:        449     886
 
PpuMI rG'GwC_Cy

Cuts at:      0     156     270    1335 
   Size:        156     114    1065

  Fragments arranged by size: 

                1065     156     114
 
PstI C_TGCA'G

Cuts at:      0     387     689    1203    1335 
   Size:        387     302     514     132

  Fragments arranged by size: 

                 514     387     302     132
 
PvuII CAG'CTG

Cuts at:      0    1009    1335 
   Size:       1009     326
 
SacI G_AGCT'C

Cuts at:      0     356    1139    1335 
   Size:        356     783     196

  Fragments arranged by size: 

                 783     356     196
 
SalI G'TCGA_C

Cuts at:      0     189    1335 
   Size:        189    1146
 
SbfI CC_TGCA'GG

Cuts at:      0     689    1335 
   Size:        689     646
 
SfcI C'TryA_G

Cuts at:      0     383     685    1199    1335 
   Size:        383     302     514     136

  Fragments arranged by size: 

                 514     383     302     136
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     169    1335 
   Size:        169    1166
 
SfoI GGC'GCC

Cuts at:      0     234    1335 
   Size:        234    1101
 
SmlI C'TyrA_G

Cuts at:      0     853    1056    1335 
   Size:        853     203     279

  Fragments arranged by size: 

                 853     279     203
 
StuI AGG'CCT

Cuts at:      0     601    1335 
   Size:        601     734
 
StyI C'CwwG_G

Cuts at:      0     596    1099    1205    1335 
   Size:        596     503     106     130

  Fragments arranged by size: 

                 596     503     130     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1165    1335 
   Size:       1165     170
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     300     332     350     382    1335 
   Size:        300      32      18      32     953

  Fragments arranged by size: 

                 953     300      32      32      18
 
Tth111I GACn'n_nGTC

Cuts at:      0     712    1335 
   Size:        712     623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     270    1335 
   Size:        270    1065
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     AlfI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI    BciVI     BglI    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrBI    BsrFI    BssSI   BstAPI    BstYI     BtgI
     BtsI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     KasI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     PasI     PfoI     PmlI    PpuMI     PstI    PvuII     SacI     SalI
     SbfI     SfcI     SfiI     SfoI     SmlI     StuI     StyI    TaqII
     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmtI     BsaI    BsiWI    BspEI    BspMI    BsrDI    BsrGI   BssHII
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     FseI     FspI    FspAI     HpaI     KpnI
     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SapI
     ScaI    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     XbaI     XhoI     XmaI     XmnI
     ZraI
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