Restriction map for pF1KB3095
 (Circular) (Six-base) MAPSORT of: pF1KB3095.seq  Check: 8191  from: 1  to: 4094



pF1KB3095  4094 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AatII G_ACGT'C

Cuts at:   1047    1047 
   Size:       4094
 
AccI GT'mk_AC

Cuts at:    310    1132     310 
   Size:        822    3272
 
Acc65I G'GTAC_C

Cuts at:   1110    1110 
   Size:       4094
 
AclI AA'CG_TT

Cuts at:   3934    3934 
   Size:       4094
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    218    1919    2351    3098    3366     218 
   Size:       1701     432     747     268     946

  Fragments arranged by size: 

                1701     946     747     432     268
 
AflII C'TTAA_G

Cuts at:    465     465 
   Size:       4094
 
AflIII A'CryG_T

Cuts at:   1446    2550    3527    1446 
   Size:       1104     977    2013

  Fragments arranged by size: 

                2013    1104     977
 
AgeI A'CCGG_T

Cuts at:   2482    2482 
   Size:       4094
 
AhdI GACnn_n'nnGTC

Cuts at:   1048    1048 
   Size:       4094
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1056    1088    1056 
   Size:         32    4062
 
AlwNI CAG_nnn'CTG

Cuts at:   1399    2966    1399 
   Size:       1567    2527
 
ApaLI G'TGCA_C

Cuts at:   2864    2864 
   Size:       4094
 
ApoI r'AATT_y

Cuts at:    284     805     871    1035    1098    3447    3703     284 
   Size:        521      66     164      63    2349     256     675

  Fragments arranged by size: 

                2349     675     521     256     164      66      63
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4094
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4094
 
AvaI C'yCGr_G

Cuts at:    230    1114     230 
   Size:        884    3210
 
BamHI G'GATC_C

Cuts at:   1119    1119 
   Size:       4094
 
BanI G'GyrC_C

Cuts at:   1110    1524    1772    1807    1110 
   Size:        414     248      35    3397

  Fragments arranged by size: 

                3397     414     248      35
 
BanII G_rGCy'C

Cuts at:    846    1108    2138     846 
   Size:        262    1030    2802

  Fragments arranged by size: 

                2802    1030     262
 
BbeI G_GCGC'C

Cuts at:   1528    1776    1528 
   Size:        248    3846
 
BbsI GAAGACnn'nnnn_

Cuts at:    559     559 
   Size:       4094
 
BciVI GTATCCnnnnn_n'

Cuts at:   1992    2753    4094    1992 
   Size:        761    1341    1992

  Fragments arranged by size: 

                1992    1341     761
 
BclI T'GATC_A

Cuts at:   1614    1614 
   Size:       4094
 
BglII A'GATC_T

Cuts at:    236     608    1609    3646     236 
   Size:        372    1001    2037     684

  Fragments arranged by size: 

                2037    1001     684     372
 
BlpI GC'TnA_GC

Cuts at:   1206    1206 
   Size:       4094
 
Bme1580I G_kGCm'C

Cuts at:   1719    1812    2868    1719 
   Size:         93    1056    2945

  Fragments arranged by size: 

                2945    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1306    1387    1722    3866    1306 
   Size:         81     335    2144    1534

  Fragments arranged by size: 

                2144    1534     335      81
 
BmtI G_CTAG'C

Cuts at:    491     491 
   Size:       4094
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2461    2461 
   Size:       4094
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     175 
   Size:       4094
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1270    2149    2641    2939    1270 
   Size:        879     492     298    2425

  Fragments arranged by size: 

                2425     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    669    3798     669 
   Size:       3129     965
 
BsaAI yAC'GTr

Cuts at:   1447    1447 
   Size:       4094
 
BsaBI GATnn'nnATC

Cuts at:      6    1608    3645       6 
   Size:       1602    2037     455

  Fragments arranged by size: 

                2037    1602     455
 
BsaHI Gr'CG_yC

Cuts at:   1044    1525    1773    2475    3971    1044 
   Size:        481     248     702    1496    1167

  Fragments arranged by size: 

                1496    1167     702     481     248
 
BsaWI w'CCGG_w

Cuts at:   1283    1804    2482    2756    2903    3314    1283 
   Size:        521     678     274     147     411    2063

  Fragments arranged by size: 

                2063     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    368     398     779     809     368 
   Size:         30     381      30    3653

  Fragments arranged by size: 

                3653     381      30      30
 
BseYI C'CCAG_C

Cuts at:    749    1519    2277    2854    3350     749 
   Size:        770     758     577     496    1493

  Fragments arranged by size: 

                1493     770     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    208     208 
   Size:       4094
 
BsiEI CG_ry'CG

Cuts at:    104    1636    1682    2890     104 
   Size:       1532      46    1208    1308

  Fragments arranged by size: 

                1532    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    846    1108    1886    2076    2868     846 
   Size:        262     778     190     792    2072

  Fragments arranged by size: 

                2072     792     778     262     190
 
BsmI GAATG_Cn'

Cuts at:    526     526 
   Size:       4094
 
Bsp1286I G_dGCh'C

Cuts at:    846    1108    1719    1812    1886    2076    2138    2868     846 
   Size:        262     611      93      74     190      62     730    2072

  Fragments arranged by size: 

                2072     730     611     262     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3314    3314 
   Size:       4094
 
BspHI T'CATG_A

Cuts at:    819    3270     819 
   Size:       2451    1643
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    650    1144    1358    1660    2041     650 
   Size:        494     214     302     381    2703

  Fragments arranged by size: 

                2703     494     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2386    2440    3924    4089      50 
   Size:       2336      54    1484     165      55

  Fragments arranged by size: 

                2336    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2006    2006 
   Size:       4094
 
BsrFI r'CCGG_y

Cuts at:   2092    2273    2482    2092 
   Size:        181     209    3704

  Fragments arranged by size: 

                3704     209     181
 
BssSI C'ACGA_G

Cuts at:   2365    2723    2365 
   Size:        358    3736
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1471    1471 
   Size:       4094
 
BstBI TT'CG_AA

Cuts at:   2455    2455 
   Size:       4094
 
BstEII G'GTnAC_C

Cuts at:    894     894 
   Size:       4094
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1027    1027 
   Size:       4094
 
BstYI r'GATC_y

Cuts at:      1     236     608    1119    1586    1609    1944    2190    3202 
   Size:        235     372     511     467      23     335     246    1012

Cuts at:   3202    3288    3300    3646       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     511     467     449     372     346     335     246
                 235      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    311     311 
   Size:       4094
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4094
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2044    2225    2388    2044 
   Size:        181     163    3750

  Fragments arranged by size: 

                3750     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1337    1337 
   Size:       4094
 
DraI TTT'AAA

Cuts at:   1093    1093 
   Size:       4094
 
DrdI GACnn_nn'nnGTC

Cuts at:   1800    2658    1800 
   Size:        858    3236
 
EaeI y'GGCC_r

Cuts at:   1679    1853    2244    2271    3616    1679 
   Size:        174     391      27    1345    2157

  Fragments arranged by size: 

                2157    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1679    1679 
   Size:       4094
 
EarI CTCTTCn'nnn_

Cuts at:   2117    2327    2117 
   Size:        210    3884
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    1064    2302    2612    2758    3904     127 
   Size:        937    1238     310     146    1146     317

  Fragments arranged by size: 

                1238    1146     937     317     310     146
 
EcoICRI GAG'CTC

Cuts at:    844    1106     844 
   Size:        262    3832
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     218    1919    2351    3098    3366     175 
   Size:         43    1701     432     747     268     903

  Fragments arranged by size: 

                1701     903     747     432     268      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:    695     695 
   Size:       4094
 
EcoO109I rG'GnC_Cy

Cuts at:   1233    1233 
   Size:       4094
 
EcoRI G'AATT_C

Cuts at:   1035    1098    1035 
   Size:         63    4031
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2113    2113 
   Size:       4094
 
FspI TGC'GCA

Cuts at:   1875    1875 
   Size:       4094
 
HaeII r_GCGC'y

Cuts at:   1528    1776    2798    3777    1528 
   Size:        248    1022     979    1845

  Fragments arranged by size: 

                1845    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    631     663    1034    1066    1936    1966    1968    1998     631 
   Size:         32     371      32     870      30       2      30    2727

  Fragments arranged by size: 

                2727     870     371      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1133    1133 
   Size:       4094
 
KasI G'GCGC_C

Cuts at:   1524    1772    1524 
   Size:        248    3846
 
KpnI G_GTAC'C

Cuts at:   1114    1114 
   Size:       4094
 
MluI A'CGCG_T

Cuts at:   3527    3527 
   Size:       4094
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2765    2949    2765 
   Size:        184    3910
 
MscI TGG'CCA

Cuts at:   1855    1855 
   Size:       4094
 
MslI CAynn'nnrTG

Cuts at:    482    2083    2210     482 
   Size:       1601     127    2366

  Fragments arranged by size: 

                2366    1601     127
 
MspA1I CmG'CkG

Cuts at:    279    1205    1519    1879    2892    3137     279 
   Size:        926     314     360    1013     245    1236

  Fragments arranged by size: 

                1236    1013     926     360     314     245
 
NaeI GCC'GGC

Cuts at:   2275    2275 
   Size:       4094
 
NarI GG'CG_CC

Cuts at:   1525    1773    1525 
   Size:        248    3846
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4094
 
NgoMIV G'CCGG_C

Cuts at:   2273    2273 
   Size:       4094
 
NheI G'CTAG_C

Cuts at:    487     487 
   Size:       4094
 
NspI r_CATG'y

Cuts at:   1147    1647    2554    1147 
   Size:        500     907    2687

  Fragments arranged by size: 

                2687     907     500
 
PciI A'CATG_T

Cuts at:   2550    2550 
   Size:       4094
 
PfoI T'CCnGG_A

Cuts at:    174     174 
   Size:       4094
 
PmeI GTTT'AAAC

Cuts at:   1093    1093 
   Size:       4094
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3233    3265    3233 
   Size:         32    4062
 
PsiI TTA'TAA

Cuts at:    434     434 
   Size:       4094
 
PstI C_TGCA'G

Cuts at:   1141    1826    1141 
   Size:        685    3409
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4094
 
PvuII CAG'CTG

Cuts at:   1519    1879    1519 
   Size:        360    3734
 
RsrII CG'GwC_CG

Cuts at:   2289    2289 
   Size:       4094
 
SacI G_AGCT'C

Cuts at:    846    1108     846 
   Size:        262    3832
 
SalI G'TCGA_C

Cuts at:   1131    1131 
   Size:       4094
 
SapI GCTCTTCn'nnn_

Cuts at:   2117    2327    2117 
   Size:        210    3884
 
SbfI CC_TGCA'GG

Cuts at:   1141    1141 
   Size:       4094
 
ScaI AGT'ACT

Cuts at:   3611    3611 
   Size:       4094
 
SfcI C'TryA_G

Cuts at:     33     584    1137    1822    2815    3006      33 
   Size:        551     553     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     553     551     191
 
SfoI GGC'GCC

Cuts at:   1526    1774    1526 
   Size:        248    3846
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4094
 
SmaI CCC'GGG

Cuts at:   1116    1116 
   Size:       4094
 
SmlI C'TyrA_G

Cuts at:    465    1249    2164    2656    2918     465 
   Size:        784     915     492     262    1641

  Fragments arranged by size: 

                1641     915     784     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4094
 
SphI G_CATG'C

Cuts at:   1147    1647    1147 
   Size:        500    3594
 
SspI AAT'ATT

Cuts at:    540     540 
   Size:       4094
 
StyI C'CwwG_G

Cuts at:    107    1228    1582     107 
   Size:       1121     354    2619

  Fragments arranged by size: 

                2619    1121     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1677    1810    2269    2478    1677 
   Size:        133     459     209    3293

  Fragments arranged by size: 

                3293     459     209     133
 
TatI w'GTAC_w

Cuts at:   3609    3609 
   Size:       4094
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    170     202     368     400    3611    3643     170 
   Size:         32     166      32    3211      32     621

  Fragments arranged by size: 

                3211     621     166      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1891    1891 
   Size:       4094
 
XbaI T'CTAG_A

Cuts at:   1125    1125 
   Size:       4094
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1589    1589 
   Size:       4094
 
XmaI C'CCGG_G

Cuts at:   1114    1114 
   Size:       4094
 
XmnI GAAnn'nnTTC

Cuts at:    556     610     556 
   Size:         54    4040
 
ZraI GAC'GTC

Cuts at:   1045    1045 
   Size:       4094
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
     PciI     PfoI     PmeI     PpiI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AleI     AlfI     ApaI     AscI    AvrII     BaeI
    BbvCI     BcgI    BfrBI     BglI    BmgBI   Bpu10I    BseRI    BsiWI
    BsmBI    BsrGI   BssHII   Bsu36I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI
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